11 Nooc oo Cudurrada Neefsashada ah

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa ogaanshaha tayada ee in vitro ee cudurada caadiga ah ee neefsashada ee ku dhaca xaakada aadanaha, oo ay ku jiraan Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonas maltophilia (Smet), Bordetella pertussis (BP), Bacillus parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Lug). Natiijooyinka waxaa loo isticmaali karaa gargaar ahaan ogaanshaha bukaanada isbitaalka la dhigay ama kuwa aadka u xanuunsan ee qaba caabuqyada bakteeriyada ee marinnada neefsashada.Qalabkan waxaa loo isticmaalaa ogaanshaha tayada ee in vitro ee cudurada caadiga ah ee neefsashada ee ku dhaca xaakada aadanaha, oo ay ku jiraan Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonas maltophilia (Smet), Bordetella pertussis (BP), Bacillus parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Lug). Natiijooyinka waxaa loo isticmaali karaa gargaar ahaan ogaanshaha bukaanada isbitaalka la dhigay ama kuwa aadka u xanuunsan ee qaba caabuqyada bakteeriyada ee marinnada neefsashada.

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Faahfaahinta Badeecada

Calaamadaha Alaabta

Magaca alaabta

HWTS-RT162A 11 Nooc oo ah Qalabka Ogaanshaha Aashitada Nukliyeerka(PCR-ka Fluorescence-ka)

Kanaal

靶标				Kanaal
核酸反应液A	核酸反应液B	核酸反应液C	核酸反应液D	Kanaal
HI	Smet	MP	Lug	FAM
SP	PA	Bp	/	CY5
KPN	ABA	Bpp	Cpn	ROX
内参	内参	内参	内参	VIC/HEX

Cudurrada Faafa

Caabuqyada marinnada neefsashadu waa nooc muhiim ah oo cudurro ah oo khatar weyn ku ah caafimaadka aadanaha, daraasaduhuna waxay muujiyeen in inta badan caabuqyada marinnada neefsashadu ay sababaan jeermisyada bakteeriyada iyo fayraska oo si wadajir ah u qaadsiiya martida, taasoo horseedaysa darnaanta cudurka iyo xitaa dhimashada, sidaa darteed caddeynta cudur-sidaha waxay u oggolaanaysaa daaweyn la beegsanayo waxayna wanaajisaa heerka badbaadada bukaanka.^[1,2].

Halbeegyada Farsamada

Kaydinta	≤-18℃
Muddada khaanadaha	12 bilood
Nooca Muunada	Xaako
Ct	HI, SP, KPN, PA, ABA, Smet: Ct≤33BP, Bpp, MP, Cpn, Lug: Ct≤38
CV	<5.0%
LoD	Klebsiella oof wareenka, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasaeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia iyo LegionellaOof-wareen: 1000 CFU/mL； Bordetella pertussis iyo Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae iyo Chlamydia pneumoniae: 200 nuqul/mL.
Gaar ahaanshaha	Natiijooyinka baaritaanka isku-dhafka fal-celinta ayaa muujiyay in aysan jirin wax falcelin is-dhaafsi ah oo u dhexeeya qalabkan iyo Cytomegalovirus, fayraska Herpes simplex nooca 1, fayraska Varicella-zoster, fayraska Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, noocyada la yareeyay ee qaaxada Mycobacterium, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivaryus, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii iyo asiidhyada hidde-sidayaasha aadanaha.
Qalabka Khuseeya	Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio^®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P LightCycler^®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96

Socodka Shaqada

Qalabka Guud ee Baaritaanka Macro & Micro-Test ee DNA/RNA (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 200µL oo ahfiisiyoolajikmilixda ku shub biyo-mareenka la farsameeyay, tallaabooyinka xigana waa in la sameeyaa iyadoo la raacayo tilmaamaha. Mugga lagu taliyay ee lagu shubay biyaha waa 100 µL.

Kii hore: Isbeddelka Hidde-sidaha Isku-dhafka ah ee Aadanaha PML-RARA

Xiga: Isbeddelka Hidde-sidaha ee ALDH

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