15 Nooc oo ah fayraska papillomavirus ee khatarta sare leh ee aadanaha E6/E7 hiddo-wadaha mRNA
Magaca alaabta
Noocyada HWTS-CC005A-15 ee Qalabka Ogaanshaha Hiddaha ee Papillomavirus-ka Aadanaha ee Khatarta Sare leh E6/E7 (Fluorescence PCR)
Cudurrada Faafa
Kansarka ilmo-galeenka waa mid ka mid ah noocyada ugu badan ee kansarka dheddigga adduunka oo dhan, dhacdadiisuna waxay si dhow ula xiriirtaa fayraska papilloma-da aadanaha (HPV), laakiin qayb yar oo ka mid ah caabuqyada HPV ayaa isu beddeli kara kansar. HPV-ga khatarta sare leh wuxuu ku dhacaa unugyada epithelial-ka ilmo-galeenka wuxuuna soo saaraa laba oncoprotein, E6 iyo E7. Borotiinkani wuxuu saameyn karaa noocyo kala duwan oo borotiinno unugyo ah (sida borotiinnada xakamaynta burooyinka pRB iyo p53), wuxuu dheereyn karaa wareegga unugyada, wuxuu saameyn karaa isku-darka DNA-da iyo xasilloonida hidde-sideyaasha, wuxuuna faragelin karaa jawaabaha difaaca jirka ee fayraska iyo ka-hortagga burooyinka.
Kanaal
| Kanaal | Qaybta | Genotype-ka la baaray |
| FAM | Kaydka Falcelinta HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Hidde-sidaha β-actin ee aadanaha | |
| FAM | Kaydka Falcelinta HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Hidde-sidaha INS-ka Aadanaha |
Halbeegyada Farsamada
| Kaydinta | Dareere: ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | Suufka ilmo-galeenka |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Nuqul/mL |
| Qalabka Khuseeya | Nidaamka PCR-ga ee Waqtiga-dhabta ah ee La Adeegsaday ee Biosystems 7500Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha isticmaalka. Mugga dheecaanka lagu taliyay waa 50μL. Haddii muunaddu aanay si buuxda u dheefshiidin, ku celi tallaabada 4 si aad dib ugu soo celiso. Kadibna tijaabi sida waafaqsan tilmaamaha isticmaalka.
Waxyaabaha lagu taliyay ee soo saarista: RNAprep Pure Animal Nudaha Tissue Total RNA (DP431). Soo saarista waa in loo sameeyaa sida waafaqsan tilmaamaha si adag loo isticmaalo (Tallaabada 5, labanlaab xoogga xalka shaqada DNaseI, taas oo ah, qaado 20μL oo ah xalka kaydka DNase-Free DNaseI (1500U) tuubo centrifuge cusub oo RNase-Free ah, ku dar 60μL oo ah RDD buffer, oo si tartiib ah isku qas). Mugga soo saarista ee lagu taliyay waa 60μL. Haddii muunaddu aanay si buuxda u dheefshiidin, ku celi tallaabada 5 si aad dib ugu soo celiso. Kadibna tijaabi sida waafaqsan tilmaamaha isticmaalka.
