15 nooc oo khatarta halista ah ee qatarta sareeya ee papillomavirus e6 / e7 hidde Mrna

15 nooc oo khatarta halista ah ee bini-aadamka ee sareeya ee papillomavirus e6 / e7 hidde-ka-hiddo-wadaha

Sharraxaadda Gaaban:

Qalabkan waxaa loogu talagalay in la ogaado tayada tayada ee 15 qatar ah oo qatar ah E6 / E7 heerar hiddo-wadeyaal ah oo muujinaya unugyada dillaacay ee makaanka afkiisa.

U dir emaylka

Si faahfaafo

Calaamadaha wax soo saarka

Magaca wax soo saarka

HWTS-CC005A-15 Noocyada Khatarta Khatarta Sare ee Papillomavirus E6 / E7 Kicinta Oncen ee Hanterka (PCR)

Nadiifin

Kansarka afka ilmo-galeenku waa mid ka mid ah noocyada ugu caansan ee kansarka dumarka ee adduunka oo dhan, dhacdadeedana waxay si dhaw ulaqabsanaysaa papillomavirus-ka bini-aadamka (HPV), laakiin qayb yar oo ah infakshannada HPV waxay ku dhici karaan kansarka. Khatarta HPV-ga sare waxay ku dhacdaa unugyada xubinta taranka ee afka ilmo-galeenka waxayna soo saartaa laba oncoproteins, E6 iyo E7. Borotiinkani wuxuu saameyn ku yeelan karaa borotiinno yar yar oo ah borotiinno (sida borotiinka bustaha bahalada ee P53), dheereynta wareegga unugyada, waxay saameyn ku yeeshaan isku-darka unugyada iyo xasilloonida guud, oo ay farageliyaan jawaabaha antiviral iyo kuwa ka hortaga dhimashada.

Kanaal

Kanaal	Qayb	Genotype tijaabiyey
Fa farqad	HPV Falcelinta Falcelinta Buffer 1	HPV16,31,33,35,55,51,52,52,58
Vic / hex	HPV Falcelinta Falcelinta Buffer 1	Biyaha β-active hidde
Fa farqad	HPV Falcelinta Falcelinta Buffer 2	HPV 18,39,45,53,53,56,59,59,66,68
Vic / hex	HPV Falcelinta Falcelinta Buffer 2	Caadada Bini-aadamka

Cayayaanka farsamada

Bakhaar	Dareere: ≤-18 ℃
Shelf-Life	9 bilood
Nooca muunadda	Suuxsan
Ct	≤38
CV	<5.0%
Dhigid	500 nuqul / ml
Aaladaha lagu dabaqi karo	Dosystems-ka lagu dabaqay 7500 Nidaamka PCR-ka dhabta ahWaxaa lagu dabaqay bio-yariyada bio-yada lagu dabaqay 7500 oo si dhakhso leh u ah nidaamyada PCR-ga Jadwalka PCRSSDUDDUDO®5 Nidaamyada PCR Nidaamyada 'Slacan-96p' ee waqtiga dhabta ah ee PCR Nalalka Firfircoon480 Nidaamka PCR-ka waqtiga-dhabta ah Sadarka 9600 oo lagu daray nidaamka ogaanshaha PCR-ka waqtiga-dhabta ah MA-6000 baaskiil-ku-meel-gaadhka waqtiga-ku-meel-gaarka ah Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Biorad Cfx Opus 96 Nidaamka PCR-ka waqtiga-dhabta ah

Socodka shaqada

Soosaarka lagu taliyay Reaggent: Macro - Imtixaanka Micro-Micro-Imtixaanka fayraska fayraska (hwts-3020-50-hpv15 . Mugga obtiga lagu talinayo waa 50μl. Haddii muunadda aan gabi ahaanba la dheeflin, ku soo celi tallaabada 4 si aad uhesho. Ka dibna tijaabi si waafaqsan tilmaamaha loo isticmaalo.

Soosaarka lagu taliyay Reaggent: RNAPREP QODOBADA XUQUUQDA XUQUUQDA XUQUUQDA KITS RNA (DP431). Soosaarista waa in loo sameeyaa si waafaqsan tilmaamaha si adag (tallaabada 5, ay labanlaabto uruurinta xalka shaqada ee Dnata, taasina waxay ku qaadataa 20μl-flae-fleant flather-ka-bilaash ah ee tuubbada 'Centrifuge' oo bilaash ah. Ku dar 60μl oo ka mid ah buffer, oo si tartiib ah ugu dar). Mugga oraahda lagu taliyey waa 60μl. Haddii muunadda aan gabi ahaanba la dheeflin, ku soo celi tallaabada 5-aad ee dib loo dhigto. Ka dibna tijaabi si waafaqsan tilmaamaha loo isticmaalo.

Hore: Papillomavirus-ka bini-aadamka (28 nooc) gebi ahaanba

Xiga: HIV 1/2 ka hortag

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