Adenovirus Universal
Magaca alaabta
HWTS-RT017A Adenovirus Qalabka Ogaanshaha Ashitada Nukliyeerka Caalamiga ah (Fluorescence PCR)
Epidemiology
Adenovirus (HAdV) waxaa iska leh genus Mammalian adenovirus, kaas oo ah fayraska DNA-ga laba-geesoodka ah oo aan lahayn baqshad.Adenoviruses ilaa hadda la helay waxaa ka mid ah 7 koox-hoosaad (AG) iyo 67 nooc, kuwaas oo 55 serotypes ay yihiin cudur-sidaha aadanaha.Waxaa ka mid ah, keeni kara infekshannada mareenka neef-mareenka inta badan kooxda B (Nooca 3, 7, 11, 14, 16, 21, 50, 55), Kooxda C (Nooca 1, 2, 5, 6, 57) iyo kooxda E. (Nooca 4), oo u horseedi kara caabuqa shubanka mindhicirka waa kooxda F (Nooca 40 iyo 41) [1-8].Noocyo kala duwan ayaa leh calaamado caafimaad oo kala duwan, laakiin inta badan caabuqyada habka neefsashada.Cudurrada neef-mareenka ee ay sababaan caabuqyada hab-mareenka neef-mareenka ee jidhka bini'aadamka ayaa ah 5% ~ 15% cudurrada neef-mareenka ee caalamiga ah, iyo 5% -7% cudurrada neef-mareenka ee carruurnimada ee caalamiga ah.Adenovirus waxa uu ku fidsan yahay meelo kala duwan, waxaana uu ku dhici karaa sanadka oo dhan, gaar ahaan meelaha dadku ku badan yahay, kuwaas oo u nugul inuu ka dillaaco gudaha, gaar ahaan dugsiyada iyo xeryaha ciidamada.
Kanaalka
| FAM | adenovirus guud ahaannucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Nasopharyngeal suuf,Dhuunta dhuunta |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 koobi/ml |
| Gaar ahaan | a) Ku tijaabi xirmada tix-raacyada xunxun ee shirkadda heerka caadiga ah, natiijaduna waxay buuxisay shuruudaha. b) Isticmaal xirmadan si aad u ogaato oo aanay jirin wax falcelin ah oo ka imanaysa cudur-sidaha kale ee neef-mareenka (sida Fayraska Hargabka A, Fayraska Hargabka B, Fayraska isku dhafka ah ee neefsiga, fayraska Parainfluenza, Rhinovirus, Metapneumovirus dadka, iwm.) ama bakteeriyada (Streptococcus pneumoniae). Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, iwm). |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshahas (FQD-96A, HangzhouTignoolajiyada bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamyada PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamyada PCR-waqtiga-dhabta ah |
Socodka Shaqada
(1) Soo saarista lagu taliyay:Macro & Tijaabada-yar ee Tusaalaha Siideynta Reagent (HWTS-3005-8).Soo saarista waa in lagu sameeyaa si waafaqsan tilmaamaha.Muunada la soo saaray waa bukaannada'suufka sanka ama sanbabada dhuunta dhuunta oo laga ururiyo goobta.Ku dar shaybaarada muunada sii deynta reagent by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex si aad si fiican isugu walaaqdo, dhig heerkulka qolka 5 daqiiqo, soo saar ka dibna rogo oo si fiican u qas si aad u hesho DNA-da muunad kasta.
(2) Soo saarista lagu taliyay:Imtixaanka Macro & Micro-Test Viral Qalabka DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Soo saareyaasha Asiidhka Nucleic-ka ah (HWTS-3006C, HWTS-3006B).qaliinka waa in lagu sameeyaa si adag oo waafaqsan tilmaamaha.Mugga muunadda la soo saaray waa 200μL, iyolagu taliyay mugga sareyntais80μL.
(3) Soo saarista lagu taliyay: Soo saarista Asiidhka Nukliyeerka ama Reagent nadiifinta (YDP)315Waxaa qoray Tiangen Biotech (Beijing) Co., Ltd., ahqalliinka waa in lagu sameeyaa si adag oo waafaqsan tilmaamaha.Mugga muunadda la soo saaray waa 200μL, iyolagu taliyay mugga sareyntais80μL.
