Isbeddelka Hidde-sidaha ee ALDH
Magaca alaabta
HWTS-GE015Qalabka Ogaanshaha Isbeddelka Hidde-sidaha ee ALDH (ARMS -PCR)
Cudurrada Faafa
Hidde-sidayaasha ALDH2 (acetaldehyde dehydrogenase 2), waxay ku yaalaan koromosoomka aadanaha 12. ALDH2 waxay leedahay firfircoonida esterase, dehydrogenase iyo reductase isku mar. Daraasaduhu waxay muujiyeen in ALDH2 uu yahay ensaym dheef-shiid kiimikaad oo nitroglycerin ah, kaas oo nitroglycerin u beddela nitric oxide, taasoo dejinaysa xididdada dhiigga iyo hagaajinta khalkhalka socodka dhiigga. Si kastaba ha ahaatee, waxaa jira isbeddello badan oo ku jira hidde-sidayaasha ALDH2, kuwaas oo inta badan ku urursan Bariga Aasiya. Nooca duurjoogta ah ee ALDH2*1/*1 GG wuxuu leeyahay awood dheef-shiid kiimikaad oo xooggan, halka nooca heterozygous uu leeyahay 6% oo keliya dhaqdhaqaaqa ensaymka nooca duurjoogta ah, nooca mutant-ka homozygous-na wuxuu leeyahay ku dhawaad eber firfircoonida ensaymka, iyadoo dheef-shiid kiimikaadku aad u daciif yahay oo uusan gaari karin saameynta la rabo, taasoo waxyeello u geysanaysa jirka aadanaha.
Kanaal
| FAM | ALDH2 |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Dhiigga xinjirowga lidka ku ah EDTA |
| CV | <5.0％ |
| LoD | 103Nuqullo/mL |
| Qalabka Khuseeya | Nidaamka PCR-ga ee Waqtiga-dhabta ah ee La Adeegsaday ee Biosystems 7500 Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P LightCycler®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: isticmaal Qalabka Soo saarista DNA-da ee Dhiigga Hidde-sidaha (DP318) ee Tiangen Biotech (Beijing) Co., Ltd. ama Qalabka Soo saarista Hidde-sidaha Dhiigga (A1120) ee Promega si aad u soo saarto dhiigga EDTA ee ka hortagga xinjirowga dhiigga.
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Guud ee DNA/RNA ee Macro & Micro-Test (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha. Mugga soo saarista ee lagu taliyay waa100μL.
