ALDH Genetic Polymorphism
Magaca alaabta
HWTS-GE015Qalabka lagu ogaanayo hidde-sideyaasha Polymorphism ALDH (ARMS -PCR)
Epidemiology
ALDH2 hidda-wadaha (acetaldehyde dehydrogenase 2), waxay ku taallaa koromosoomyada bini'aadamka 12. ALDH2 waxay leedahay esterase, dehydrogenase iyo firfircoonida reductase isku mar. Daraasaduhu waxay muujiyeen in ALDH2 uu yahay enzyme-ka dheef-shiid kiimikaad ee nitroglycerin, kaas oo nitroglycerin u beddelaya nitric oxide, taas oo dejisa xididdada dhiigga iyo hagaajinta cilladaha socodka dhiigga. Si kastaba ha ahaatee, waxaa jira polymorphisms hidda-wadaha ALDH2, kuwaas oo inta badan ku urursan Bariga Aasiya. Nooca duurjoogta ah ee ALDH2 * 1/* 1 GG wuxuu leeyahay awood dheef-shiid kiimikaad oo xooggan, halka nooca heterozygous uu leeyahay 6% oo kaliya ee dhaqdhaqaaqa nooca-enzyme-ga ah, iyo nooca mutant-ga ah ee homozygous wuxuu leeyahay ku dhawaad eber firfircoonida enzyme, oo leh dheef-shiid kiimikaad aad u daciif ah oo aan gaari karin saameynta la rabo, sidaas darteed waxyeello u geysta jirka bini'aadamka.
Kanaalka
| FAM | ALDH2 |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | EDTA dhiigga xinjirowga lidka ku ah |
| CV | <5.0 |
| LoD | 103Koobiyo/ml |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Dib-u-hagaajinta soo saarista lagu taliyay: isticmaal Qalabka Soo saarista DNA-ga Dhiiga Genome (DP318) ee Tiangen Biotech (Beijing) Co., Ltd. ama Qalabka Soo saarista Hidde-sidayaasha Dhiiga (A1120) ee Promega si loo soo saaro EDTA dhiigga xinjirowga lidka ku ah DNA-da.
Reagents soo saarista lagu taliyay: Macro & Micro-Test DNA General / RNA Kit (HWTS-3019) (kaas oo loo isticmaali karaa Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in si adag loo qabtaa si waafaqsan tilmaamaha. Mugga sifaynta lagu taliyey waa100μL
