Borrelia Burgdorferi Nucleic Acid
Magaca alaabta
HWTS-OT068 Borrelia Burgdorferi Qalabka Ogaanshaha Asiidhka Nucleic (Fluorescence PCR)
Shahaado
CE
Epidemiology
Cudurka Lyme waxaa keena caabuqa Borrelia burgdorferi waxaana inta badan lagu kala qaadaa inta u dhaxaysa xayawaanka martida loo yahay, inta u dhaxaysa xayawaanka martida loo yahay iyo aadanaha by shilinta adag.Cudurka loo yaqaan 'pathogen' Borrelia burgdorferi wuxuu sababi karaa soogalootiga Erythema chronicum bini'aadamka, iyo sidoo kale cudurro ku lug leh nidaamyo badan sida wadnaha, neerfaha, iyo lafaha, iwm., astaamaha caafimaadkuna waa kala duwan yihiin.Marka loo eego horumarinta koorsada cudurrada, waxaa loo qaybin karaa caabuq hore oo gudaha ah, infekshin dhexdhexaad ah oo faafay iyo caabuq daba-dheeraaday, kuwaas oo waxyeello weyn u leh caafimaadka dadweynaha.Sidaa darteed, ogaanshaha bukaan-socodka ee Borrelia burgdorferi, waa muhiimad weyn in la sameeyo hab fudud, gaar ah oo degdeg ah oo loogu talagalay ogaanshaha etiological ee Borrelia burgdorferi.
Kanaalka
| FAM | DNA-da Borrelia burgdorferi |
| VIC/HEX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Muunadda dhiigga oo dhan |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/ml |
| Qalabka lagu dabaqi karo | ABI 7500 Nidaamyada PCR-waqtiga-dhabta ah ABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
QIAamp DNA Dhiigga Midi Kit ee Qiagen (51185).It waa in la soo saarosi adag loo raacoilaa edbinta, iyo mugga ellition ee lagu taliyey waa100μL
Doorashada 2.
DhiigGDNA-da enomicExirmada soo saarista (DP318,MayaJingchangDiiwaanka Qalabka20210062) waxaa soo saaray Tiangen Biochemical Technology (Beijing) Co., Ltd.. It waa in la soo saarosi adag loo raacoilaa edbinta, iyo mugga ellition ee lagu taliyey waa100μL
Doorashada 3.
Qalabka Nadiifinta DNA ee Wizard® Genomic (A1120) ee Promega.It waa in la soo saarosi adag loo raacoilaa edbinta, iyo mugga ellition ee lagu taliyey waa100μL
