Hiddo-sidayaasha iska caabbinta Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Magaca alaabta
Qalabka Ogaanshada ee HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (Fluorescence PCR)
Cudurrada Faafa
Antibiyootikada Carbapenem waa antibiyootikada β-lactam ee aan caadiga ahayn oo leh noocyada ugu ballaaran ee bakteeriyada dila iyo firfircoonida bakteeriyada dila ee ugu xooggan. Sababtoo ah xasilloonideeda β-lactamase iyo sunta yar, waxay noqotay mid ka mid ah daawooyinka ugu muhiimsan ee bakteeriyada dila ee loogu talagalay daaweynta caabuqyada bakteeriyada daran. Carbapenems aad bay u xasilloon yihiin β-lactamases-ka plasmid-ka ee la dheereeyey (ESBLs), koromosoomyada, iyo cephalosporinases-ka plasmid-ka ee la dhexdhexaadiyey (enzymes AmpC).
Kanaal
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Xakamaynta Gudaha | Xakamaynta Gudaha |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Xaako, gumeysi saafi ah, xaako malawad |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Gaar ahaanshaha | a) Qalabku wuxuu ogaadaa tixraacyada taban ee shirkadda ee caadiga ah, natiijooyinkuna waxay buuxinayaan shuruudaha tixraacyada u dhigma. b) Natiijooyinka baaritaanka fal-celinta isdhaafka ah waxay muujinayaan in qalabkani uusan lahayn fal-celin isdhaafsi ah oo lala yeesho jeermisyada kale ee neefsashada, sida Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Neefsashada adenovirus, Enterococcus, ama muunado ay ku jiraan hiddo-sideyaal kale oo daawada u adkaysta CTX, mecA, SME, SHV, TEM, iwm. c) Ka-hortagga faragelinta: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin ayaa loo xushay baaritaanka faragelinta, natiijooyinkuna waxay muujinayaan in walxaha faragelinta ee kor ku xusan aysan lahayn falcelin faragelin ah oo ku saabsan ogaanshaha hiddo-wadaha iska caabbinta carbapenem KPC, NDM, OXA48, OXA23, VIM, iyo IMP. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.) Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A，HangzhouTiknoolajiyada bayoolaji) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaarka 1aad.
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Guud ee DNA/RNA ee Macro & Micro-Test (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ku dar 200μL oo cusbo caadi ah oo ku jirta dareeraha thallus-ka. Tallaabooyinka xiga waa inay raacaan tilmaamaha soo saarista, mugga soo saarista ee lagu taliyayna waa inuu ahaadaa100μL.
Ikhtiyaarka 2aad.
Waxyaabaha lagu taliyay ee soo saarista: Soo saarista Aashitada Nucleic ama Reagent Purification (YDP302) oo ay samaysay Tiangen Biotech (Beijing) Co., Ltd. Soo saarista waa in si adag loo bilaabaa iyadoo la raacayo tallaabada 2aad ee tilmaamaha isticmaalka (ku dar 200μL oo ah GA oo ah dareeraha thallus-ka, oo rux ilaa thallus-ku si buuxda u istaago). Isticmaal biyaha aan lahayn RNase/DNase si aad u soo saarto, mugga dheecaanka ee la soo saarayna waa 100μL.
Ikhtiyaarka 3aad.
Waxyaabaha lagu taliyay ee soo saarista: Macro & Micro-Test Sample Reagent. Muunadda xaakada waa in la dhaqo iyadoo lagu darayo 1mL oo cusbo caadi ah oo ku jirta thallus-ka la daweeyay ee kor ku xusan, oo lagu centrifuged 13000r/daqiiqo muddo 5 daqiiqo ah, ka dibna dheecaanka waa la tuuraa (ku hay 10-20µL oo dheecaanka dheeraadka ah). Wixii suufka gumeysiga iyo malawadka saafiga ah, ku dar 50μL oo ah walxaha sii daaya muunadda si toos ah ugu shubka thallus-ka la daweeyay ee kor ku xusan, tallaabooyinka xigana waa in la soo saaraa iyadoo loo eegayo tilmaamaha isticmaalka.
