Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Magaca alaabta
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Qalabka lagu ogaado (Fluorescence PCR)
Epidemiology
Antibiyootigyada Carbapenem waa antibiyootig β-lactam oo aan caadi ahayn oo leh spectrum bakteeriyada ugu ballaadhan iyo waxqabadka bakteeriyada ugu xooggan.Sababtoo ah xasiloonida β-lactamase iyo sunta hoose, waxay noqotay mid ka mid ah daawooyinka bakteeriyada ugu muhiimsan ee daaweynta caabuqyada bakteeriyada daran.Carbapenems aad bay u deggan yihiin β-lactamases (ESBLs), koromosoomyada, iyo cephalosporinases plasmid-dhexdhexaadin ah (AmpC enzymes).
Kanaalka
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Xakamaynta Gudaha | Xakamaynta Gudaha |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Xaako, deegaan saafi ah, suufka malawadka |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/ml |
| Gaar ahaan | a) Qalabku wuxuu ogaanayaa tixraacyada xun ee shirkadda caadiga ah, natiijooyinkuna waxay buuxiyaan shuruudaha tixraacyada u dhigma. b) Natiijooyinka tijaabada falcelinta falcelinta waxay muujineysaa in xirmadani aysan lahayn falcelin iskutallaab ah oo leh cudur-sidaha kale ee neef-mareenka, sida Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Kleb junii, Acinetobacter hemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, adenovirus neefsiga, Enterococcus, ama muunado ka kooban hiddo-side kale u adkaysta daroogada CTX, mecA, TEM, SHV, iwm. c) Ka-hortagga faragelinta: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin ayaa loo doortay baaritaanka faragelinta, natiijadu waxay muujineysaa in falcelinta kor ku xusan aysan lahayn walxaha faragelinta. si loo ogaado hiddo-wadaha iska caabbinta carbapenem KPC, NDM, OXA48, OXA23, VIM, iyo IMP. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ahFQD-96A,HangzhouTignoolajiyada bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Soo saarista lagu taliyay: Makro & Tijaabada Guud ee DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ku dar 200μL ee cusbada caadiga ah Da'da thallus.Tallaabooyinka xiga waa in ay raacaan tilmaamaha soo saarista, iyo mugga elution ee lagu taliyey waa100μL
Doorashada 2.
Soo saarista lagu taliyay: Soosaarka Asiidhka Nucleic ama Reagent Nadiifinta (YDP302) ee Tiangen Biotech (Beijing) Co., Ltd. Soo saarista waa in lagu bilaabo si adag si waafaqsan talaabada 2 ee tilmaamaha isticmaalka , oo rux ilaa thallus gabi ahaanba la hakiyo).U isticmaal biyaha bilaashka ah ee RNase/DNase si aad u nadiifiso, iyo mugga elutionka ee la amaanay waa 100μL.
Doorashada 3.
Soo saarista lagu taliyay: Makro & Tijaabada Muunadda-yar ee Soo-dejinta Reagent.Muunada xaakada waxa loo baahan yahay in la maydho iyada oo lagu darayo 1mL oo cusbo ah oo caadi ah thallus precipitate ee aan kor ku soo xusnay, lagu daaweeyay 13000r/min 5 daqiiqo, ka sarreeyana waa la tuurayaa (ka dhig 10-20µL oo sarreen ah).Gumeysiga saafiga ah iyo suufka malawadka, ku dar 50μL oo muunad muunad reagent ah si toos ah kor ku xusan ee thallus precipitate ee la daweeyay, talaabooyinka xiga waa in la soo saaraa si waafaqsan tilmaamaha isticmaalka.
