Cadaadiska Carbapeence Cadditing (KPC / NDM / Ox 48 / Ox) / Vim / Vim)
Magaca wax soo saarka
HWTS-OT045 Carbapeence Resistem (KPC / NDM / OXA 48 / Ox / Vim / Vim / Spch)
Nadiifin
Antibiyootigyada Carbapeenem waa antibiyootig antibiil β-lact oo aan caadi ahayn oo leh bakteeriyada bakteeriyada bakteeriyada bakteeriyada bakteeriyada ee ugu badan ee bakteeriyada bakteeriyada ugu adag. Sababta oo ah xasilloonideeda ay u leedahay β -actamese iyo sunta hoose, waxay noqotay mid ka mid ah dawooyinka ugu muhiimsan ee bakteeriyada bakteeriyada ugu muhiimsan ee daaweynta jeermiska bakteeriyada daran. Carbapeens-yada aad ayey u xasilloon yihiin in lagu dheereeyo plasmid-ka-dheelitiran-sp spect β-lactasas (esbls), koromosoommomes, iyo plasmid-dhexdhexaadin ah chopalosporossuross (ampc enzymes).
Kanaal
| Pcr-isku dar 1 | Pcr-isku dar 2 | |
| Fa farqad | Sooloouun | Rajab ah |
| Vic / hex | Kontoroolka gudaha | Kontoroolka gudaha |
| Cy5 | Ndm | KPC |
| Rox | Oxa48
| Oxa23 |
Cayayaanka farsamada
| Bakhaar | ≤-18 ℃ |
| Shelf-Life | 12 bilood |
| Nooca muunadda | Xaako, gumeysi saafi ah, malawalba malax |
| Ct | ≤36 |
| CV | ≤5.0% |
| Dhigid | 103CFU / ML |
| Si gaar ah | a) Qalabka ayaa ogaadaa shirkadaha caadiga ah ee tixraacyada taban, natiijooyinkana waxay buuxinayaan shuruudaha tixraacyada u dhigma. b) Natiijooyinka baaritaanka isgoyska ee isgoyska ee isgoysyada ah waxay muujinayaan in xirmadaan uusan ficil celin u lahayn cudurada kale ee neef-mareenka, sida Klepomonas Aeruginona, Neisseloconscescosa, Neisseloconscoscus, Staphylococcus aureus, klesviella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, or samples containing other drug-resistant genes CTX, mecA, SME, Shv, tem, iwm. c) Faragelinta: Mucin, Mooncline, GlatBamycin, IMINAPAZONE, ROXITHOMYO, Clavutromne, oo natiijadu waxay muujinayaan in walxaha faragelinta ee kor ku xusan aysan lahayn falcelin soo-galka ah ogaanshaha Cadaadiska 'Carbapenem Rese's KPC, NDM, Oxa48, Oaa23, Vim, iyo imp. |
| Aaladaha lagu dabaqi karo | Waxaa lagu dabaqay biosystemy-ga ee lagu dabaqay 7500 nidaamyada waqtiga-dhabta ah ee PCR Jadwalka PCRSSDUDDUDO®5 Nidaamyada PCR Nidaamyada Slan-96p ee waqtiga-labaad ee PCR (tikniyoolajiyadda caafimaadka ee Hongshi ee Co., Ltd.) Nalalka Firfircoon480 Nidaamka PCR-ka waqtiga-dhabta ah Sadarka 9600 oo lagu daray nidaamka ogaanshaha waqtiga-labaad ee PCR-ka (Fqd-96a,HangzhouTeknolojiyadda bioer) MA-6000 DIIWAAN GELINTA EE MADAXWEYNAHA (SUZHOU MolARYADA CODKA, LTD.) Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Biorad Cfx Opus 96 Nidaamka PCR-ka waqtiga-dhabta ah |
Socodka shaqada
Xulashada 1.
Soosaarista lagu taliyay Reagent: Macro & Micro-Picho-General General DNA / RNA KIT (HWTS-3019-50, hwts-3019-32, hwts-3019-48, hwts-3019-96) (oo loo isticmaali karo macro-micro-macro-otomatoctic otomatoractor (hwts-3006c, hwts-3006b) oo uu ku jiro Jiangsu Macro & Tech Tech Cogn-Tech Co. .. Ku dar 200μl oo cusbo caadi ah Thallus ayaa saadaaliyay. Talaabooyinka ku xiga waa inay raacaan tilmaamaha ku saabsan soo saarista, iyo mugga obtiga lagu ogeysiiyo waa100μl.
Xulashada 2.
Soosaarista lagu taliyay , oo rux ilaa uu ka hortago gabi ahaanba laga joojiyo). U adeegso biyo-xorta / Dense bilaash ah si aad u feejignaato, oo mugga kahoreyka is-ilaalintu waa 100μl.
Xulashada 3.
Soo saarista soo saarista: Muunadda loo yaqaan 'Macro-Tijaabada' ee 'Macro-Tijaabada' Reagent. Muunadda xaakada ayaa loo baahan yahay in lagu maydho iyadoo lagu daro 1ml cusbo caadi ah oo lagu soo rogay ee kor ku xusan Thaluslus ee kor lagu soo xusay Thallus ee kor lagu soo sheegay, oo ah 5000r / Ming 5 daqiiqo, ku hay 10-20μμl oo ah superankant). Guryaha saafiga ah iyo malawadka suufka, ku dar 50μl oo muunad ah oo si toos ah ugu soo celiya si toos ah loola dhaqmo Thallus-ee kor lagu soo sheegay, iyo tillaabooyinka xiga waa in loo soosaaraa iyadoo loo eegayo tilmaamaha isticmaalka.
