Coxsackie Virus Nooca A16 Nucleic Acid

Qalabkani wuxuu qaataa Enzymatic Probe Isothermal Amplification (EPIA), wuxuuna naqshadeeyaa asal gaar ah iyo RNA-saldhig ka kooban baaritaanno (rProbe) oo loogu talagalay gobolka aadka loo ilaaliyo ee Cox A16, wuxuuna ku darayaa Bst enzyme iyo RNaseH enzyme isku mar, taas oo bidix iyo darafyada midig ee saldhiga RNA ee rProbe waxa lagu calaamadeeyay kooxo fluorescent iyo quencher, siday u kala horreeyaan.Isticmaal dhaqdhaqaaqa DNA polymerase iyo dhaqdhaqaaqa barokaca ee Bst enzyme si aad u xoojiso bartilmaameedka lagu tijaabiyo heerkulka joogtada ah, enzyme RNaseH wuxuu jebin karaa saldhigyada RNA ee silsiladda isku-dhafka ah ee bartilmaameedka, si kooxda fluorescent iyo deminta rProbe ay yihiin kala soocaa markaas fluorescing.Intaa waxaa dheer, jajabka bidix ee saldhigga rProbe RNA ee hadhaaga ah waxaa loo isticmaali karaa asal ahaan si loo sii kordhiyo si loo sameeyo badeecad, taas oo sii ururisa alaabta.Calaamadaha fluorescent ayaa si joogto ah u urura samaynta alaabta, taas oo ogaanaysa ogaanshaha bartilmaameedka nucleic acid.