Fayraska Dengue, Fayraska Zika iyo Fayraska Chikungunya Multiplex
Magaca alaabta
Fayraska Dengue ee HWTS-FE040, Fayraska Zika iyo Fayraska Chikungunya Qalabka Ogaanshaha Aashitada Nukliyeerka ee Multiplex (Fluorescence PCR)
Cudurrada Faafa
Qandhada Dengue (DF), oo ay keento caabuqa fayraska dengue (DENV), waa mid ka mid ah cudurrada ugu faafa ee arbovirus. Habka gudbinta waxaa ka mid ah Aedes aegypti iyo Aedes albopictus. DF waxay inta badan ku badan tahay meelaha kulaylaha iyo kuwa ka hooseeya kulaylku. DENV waxay ka tirsan tahay flavivirus-ka hoostiisa flaviviridae, waxaana loo kala saari karaa 4 nooc oo serotypes ah iyadoo loo eegayo antigen-ka dusha sare. Calaamadaha kiliinikada ee caabuqa DENV waxaa ugu badan madax-xanuun, qandho, daciifnimo, ballaarinta qanjidhada limfa, leukopenia iwm., iyo dhiig-bax, shoog, dhaawac beerka ah ama xitaa dhimasho kiisaska daran. Sannadihii ugu dambeeyay, isbeddelka cimilada, magaalooyinka, horumarka degdegga ah ee dalxiiska iyo arrimo kale ayaa bixiyay xaalado degdeg ah oo ku habboon gudbinta iyo faafitaanka DF, taasoo horseedaysa ballaarin joogto ah oo aagga faafa ee DF.
Kanaal
| FAM | Aashitada nukliyeer ee DENV |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | -18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | Serum cusub |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Nuqul/mL |
| Gaar ahaanshaha | Natiijooyinka baaritaanka faragelinta ayaa muujinaya marka heerka bilirubin ee ku jira seeramku aanu ka badnayn 168.2μmol/ml, heerka hemoglobin-ka ee uu soo saaro hemolysis-ku aanu ka badnayn 130g/L, heerka dufanka dhiigga ee dhiigga aanu ka badnayn 65mmol/ml, wadarta guud ee heerka IgG ee seeramku aanu ka badnayn 5mg/mL, ma jirto wax saameyn ah oo ku yeelanaya fayraska dengue, fayraska Zika ama ogaanshaha fayraska chikungunya. Fayraska Hepatitis A, fayraska Hepatitis B, fayraska Hepatitis C, fayraska Herpes, fayraska Eastern equine encephalitis, fayraska Hantavirus, fayraska Bunya, fayraska West Nile iyo muunadaha serum-ka genomic ee aadanaha ayaa loo xushay baaritaanka iskutallaabta-falcelinta, natiijooyinkuna waxay muujinayaan in aysan jirin wax falcelin ah oo u dhexeeya qalabkan iyo jeermisyada kor ku xusan. |
| Qalabka Khuseeya | Nidaamka PCR-ga ee Waqtiga-dhabta ah ee La Adeegsaday ee Biosystems 7500 Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah LightCycler®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaarka 1aad.
Qalabka DNA/RNA ee Fayraska TIAmp (YDP315-R), soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha loo isticmaalo. Mugga muunadda la soo saaray waa 140μL, mugga lagu taliyayna waa 60μL.
Ikhtiyaarka 2aad.
Qalabka Guud ee Baaritaanka Macro & Micro-Test DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., soo saaristana waa in loo sameeyaa sida ku cad tilmaamaha loo isticmaalo. Mugga muunadda la soo saaray waa 200μL, mugga lagu taliyay ee soo saarista waa 80μL.
