Fayraska Dengue, Fayraska Zika iyo Virus Multiplex ee Chikungunya
Magaca alaabta
HWTS-FE040 Virus Dengue, Virus Zika iyo Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Qandhada Dengue (DF), oo uu keeno fayraska dengue (DENV), waa mid ka mid ah cudurrada faafa ee arbovirus ugu faafa. Dhexdhexaadinteeda gudbinta waxaa ka mid ah Aedes aegypti iyo Aedes albopictus. DF waxay inta badan ku badan tahay dhulka kuleylaha ah iyo kuwa hoose. DENV waxaa iska leh flavivirus oo hoos yimaada flaviviridae, waxaana loo kala saari karaa 4 serotypes iyadoo loo eegayo antigen-ka dusha sare. Calaamadaha kiliinikada ee caabuqa DENV inta badan waxaa ka mid ah madax-xanuun, qandho, daciifnimo, balaadhinta qanjidhada qanjidhada, leukopenia iyo iwm, iyo dhiig-baxa, shoogga, dhaawaca beerka ama xitaa dhimasho xaalado daran. Sanadihii la soo dhaafay, isbeddelka cimilada, magaalooyinka, horumarinta degdega ah ee dalxiiska iyo arrimo kale ayaa keenay xaalado degdeg ah oo ku habboon gudbinta iyo fidinta DF, taasoo keentay ballaarinta joogtada ah ee DF-ka.
Kanaalka
| FAM | DENV nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | -18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Serum cusub |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 koobi/ml |
| Gaar ahaan | Natiijooyinka baaritaanka faragelinta waxay muujinayaan in marka fiirsashada bilirubin ee serum aysan ka badneyn 168.2μmol/ml, xoojinta haemoglobin ee ay soo saarto hemolysis waa inaysan ka badneyn 130g/L, fiirsashada dufanka dhiigga aan ka badneyn 65mmol/ml, wadarta xoojinta IgG ee serum kama badna 5mg/mL, ma jiro wax saameyn ah oo ku saabsan fayraska dengue. Fayraska Cagaarshowga, Cagaarshowga B, Cagaarshowga C, Fayraska Herpes, Fayraska Equine encephalitis Eastern, Hantavirus, Bunya Virus, Virus-ka West Nile iyo shaybaarada serum genomic ee dadka ayaa loo xushay baaritaanka falcelinta falcelinta, natiijaduna waxay muujineysaa in aysan jirin falcelin udhaxeysa xirmadan iyo cudur-sidaha kor ku xusan. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Tianamp Virus DNA/RNA Kit (YDP315-R), iyo soo saarista waa in loo qabtaa si adag oo waafaqsan tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 140μL, mugga sareynta lagu taliyeyna waa 60μL.
Doorashada 2.
Macro & Micro-Tijaabada Guud ee DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) Micro-Test Med-Tech Co., Ltd., iyo soo saarista waa in loo qabtaa si waafaqsan tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 80μL.
