Enterovirus Universal, EV71 iyo CoxA16
Magaca Alaabta
HWTS-EV026B-Enterovirus Universal, EV71 iyo CoxA16 Qalabka Ogaanshaha Ashitada Nukliyeerka (Fluorescence PCR)
HWTS-EV020Y/Z-Enterovirus Universal ee qaboojiya-qallalan, EV71 iyo CoxA16 Qalabka Ogaanshaha Ashitada Nukliyeerka (Fluorescence PCR)
Shahaado
CE/MDA (HWTS-EV026)
Epidemiology
Cudurka afka-gacan-cagta (HFMD) waa cudur faafa oo ba'an oo caadi ah oo ku dhaca carruurta. Inta badan waxay ku dhacdaa carruurta da'doodu ka yar tahay 5 sano, waxayna ku keeni kartaa herpes gacmaha, cagaha, afka iyo qaybaha kale, tiro yar oo caruur ah waxay keeni kartaa dhibaatooyin ay ka mid yihiin myocarditis, bararka sambabada, meningoencephalitis aseptic, iwm.
Hadda, waxaa la helay 108 serotypes oo ah enteroviruses, kuwaas oo loo kala qaybiyay afar kooxood: A, B, C iyo D. Enteroviruses keena HFMD way kala duwan yihiin, laakiin enterovirus 71 (EV71) iyo coxsackievirus A16 (CoxA16) ayaa ah kuwa ugu badan oo ay u dheer tahay HFMD, waxay keeni kartaa halis daran oo ah habdhiska dhexe ee neerfayaasha sida flangitis.
Kanaalka
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Xakamaynta gudaha |
Qiyaasta Farsamada
| Kaydinta | Dareeraha: ≤-18℃ MadowLyophilization: ≤30℃ |
| Nolosha shelf | Dareeraha: 9 biloodLyophilization: 12 bilood |
| Nooca Tuunada | Muunada dhuunta dhuunta, dareeraha Herpes |
| Ct | ≤38 |
| CV | ≤5.0 |
| LoD | 500 koobi/ml |
| Qalabka lagu dabaqi karo | Waxay la jaan qaadi kartaa qalabka PCR-ka ee caadiga ah ee suuqa.ABI 7500 Nidaamyada PCR-waqtiga-dhabta ah ABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Wadarta Xalka PCR
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Buugga soo saarida waa in la fuliyaa si waafaqsan. Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 80μL.
Soo saarista lagu taliyay: Macro & Tijaabada Muunada Soo-dejinta Reagent (HWTS-3005-8). Soo saarista waa in lagu fuliyaa si waafaqsan buug-gacmeedka. Shaybaarada la soo saaray waa suufka oropharyngeal ama muunadaha dareeraha herpes ee bukaanada lagu ururiyay goobta. Ku dar suufyada la ururiyey si toos ah Macro & Micro-Test Sample Release Reagent, vortex oo si fiican isku walaaq, dhig heerkulka qolka 5 daqiiqo, soo saar ka dibna rogo oo si fiican isku qas si aad u hesho RNA ee muunad kasta.
Soo saarista lagu taliyay: QIAamp Viral RNA Mini Kit (52904) ee QIAGEN ama Soosaarka Ashitada Nukliyeerka ama Reagent Nadiifinta (YDP315-R). Soo saarista waa in loo fuliyaa si adag oo waafaqsan buug-gacmeedka.
