Enterovirus Universal, EV71 iyo CoxA16
Magaca Badeecada
Qalabka Baadhista Aashitada Nukliyeerka ee HWTS-EV026B-Enterovirus Universal, EV71 iyo CoxA16 (Fluorescence PCR)
HWTS-EV020Y/Z- Qalabka Ogaanshaha Aashitada Nucleic ee Enterovirus Universal, EV71 iyo CoxA16 (Fluorescence PCR)
Shahaadada
CE/MDA(HWTS-EV026)
Cudurrada Faafa
Cudurka afka-gudaha (HFMD) waa cudur faafa oo caadi ah oo ku dhaca carruurta. Inta badan wuxuu ku dhacaa carruurta ka yar 5 sano, wuxuuna ku keeni karaa herpes gacmaha, cagaha, afka iyo qaybaha kale, tiro yar oo carruur ahna waxay sababi karaan dhibaatooyin sida myocarditis, bararka sambabada, meningoencephalitis aseptic, iwm. Carruurta shaqsiga ah ee qaba cudurrada halista ah si dhakhso ah ayay u xumaadaan waxayna u nugul yihiin inay dhintaan haddii aan si dhakhso ah loo daweyn.
Waqtigan xaadirka ah, waxaa la helay 108 nooc oo ah enteroviruses, kuwaas oo loo qaybiyay afar kooxood: A, B, C iyo D. Enteroviruses-ka keena HFMD way kala duwan yihiin, laakiin enterovirus 71 (EV71) iyo coxsackievirus A16 (CoxA16) ayaa ah kuwa ugu badan marka lagu daro HFMD, waxay sababi karaan dhibaatooyin daran oo habdhiska neerfaha dhexe ah sida meningitis, encephalitis, iyo curyaanimo daran.
Kanaal
| FAM | Cudurka Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Xakamaynta gudaha |
Halbeegyada Farsamada
| Kaydinta | Dareere: ≤-18℃ Mugdi ku jiraLiofilization: ≤30℃ |
| Muddada khaanadaha | Dareere: 9 biloodLiofilization: 12 bilood |
| Nooca Muunada | Muunad suufka cunaha, dareeraha herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Nuqullo/mL |
| Qalabka Khuseeya | Waxay la mid noqon kartaa qalabka PCR-ka ee caadiga ah ee suuqa yaalla.Nidaamyada PCR-ga ee ABI 7500 ee waqtiga-dhabta ah Nidaamyada PCR-ga ee ABI 7500 ee Degdegga ah ee Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee LightCycler®480 Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Xalka PCR ee Wadarta
Waxyaabaha lagu taliyay ee soo saarista: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in la sameeyaa iyadoo la raacayo tilmaamaha tilmaamaha. Mugga muunadda la soo saaray waa 200μL, mugga soo saarista ee lagu taliyayna waa 80μL.
Daawada lagu taliyay ee soo saarista: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Soo saarista waa in la sameeyaa iyadoo la raacayo tilmaamaha tilmaamaha. Muunadaha soo saarista waa suufka afka ama muunadaha dareeraha herpes ee bukaanada lagu ururiyey goobta. Ku dar suufka la ururiyey si toos ah Macro & Micro-Test Sample Reagent, vortex oo si fiican isku walaaq, dhig heerkulka qolka muddo 5 daqiiqo ah, ka saar ka dibna rog oo si fiican isku walaaq si aad u hesho RNA-ga muunad kasta.
Waxyaabaha lagu taliyay ee soo saarista: QIAamp Viral RNA Mini Kit (52904) oo ay samaysay QIAGEN ama Nucleic Acid Extract ama Purification Reagent (YDP315-R). Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo buug-gacmeedka tilmaamaha.
