Fayraska Cagaarshowga E
Magaca alaabta
HWTS-HP006 Cagaarshowga E Virus Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Cagaarshowga E (HEV) waa fayras RNA ah oo keena dhibaatooyin caafimaad oo caalami ah. Waxay leedahay tiro badan oo martigeliyayaal ah waxayna leedahay hantida ka gudubta caqabadaha isbarbardhigga ah. Waa mid ka mid ah cudur-sidaha ugu muhiimsan ee zoonotic wuxuuna dhibaato weyn u geystaa dadka iyo duunyada. HEV inta badan waxaa lagu kala qaadaa gudbinta saxarada-afka, waxaana sidoo kale lagu kala qaadaa si toos ah embriyaha ama dhiiga. Waxaa ka mid ah, dariiqa gudbinta saxarada-afka, biyaha iyo cuntada wasakhaysan ee HEV ayaa si weyn ugu faafay, iyo khatarta caabuqa HEV ee dadka iyo xayawaanka ayaa ah mid sarreeya[1-2].
Kanaalka
| FAM | HEV nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Dhuunta dhuunta |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/μL |
| Gaar ahaan | Cagaarshowga E (HEV) waa fayras RNA ah oo keena dhibaatooyin caafimaad oo caalami ah. Waxay leedahay tiro badan oo martigeliyayaal ah waxayna leedahay hantida ka gudubta caqabadaha isbarbardhigga ah. Waa mid ka mid ah cudur-sidaha ugu muhiimsan ee zoonotic wuxuuna dhibaato weyn u geystaa dadka iyo duunyada. HEV inta badan waxaa lagu kala qaadaa gudbinta saxarada-afka, waxaana sidoo kale lagu kala qaadaa si toos ah embriyaha ama dhiiga. Waxaa ka mid ah, dariiqa gudbinta saxarada-afka, biyaha iyo cuntada wasakhaysan ee HEV ayaa si weyn ugu faafay, iyo khatarta caabuqa HEV ee dadka iyo xayawaanka ayaa ah mid sarreeya[1-2]. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Codsaday Nidaamyada noolaha 7500 Nidaamka PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1
Makro & Micro-Tijaabada Guud ee DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) iyo Macro & Micro-Tijaabo toos ah Nucleic Acid Soosaarayaasha (HWTS-3006C, HWTS-3006). Waa in lagu soo saaraa si waafaqsan tilmaamaha. Mugga sifaynta lagu taliyey waa 80µL.
Doorashada 2
TIANAmp Virus DNA/RNA Kit (YDP315-R) oo ay samaysay Tiangen Biotech (Beijing) Co., Ltd. Waa in si adag loo soo saaro si waafaqsan tilmaamaha. Mugga saamiga la soo saaray waa 140 μL. Mugga caddaynta lagu taliyey waa 60µL.v
