Fayraska Cagaarshowga E
Magaca alaabta
HWTS-HP006 Cagaarshowga E Virus Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Cagaarshowga E (HEV) waa fayras RNA ah oo keena dhibaatooyin caafimaad oo caalami ah.Waxay leedahay tiro badan oo martigeliyayaal ah waxayna leedahay hantida ka gudubta caqabadaha isbarbardhigga ah.Waa mid ka mid ah cudur-sidaha ugu muhiimsan ee zoonotic wuxuuna dhibaato weyn u geystaa dadka iyo duunyada.HEV inta badan waxaa lagu kala qaadaa gudbinta saxarada-afka, waxaana sidoo kale lagu kala qaadaa si toos ah embriyaha ama dhiiga.Waxaa ka mid ah, dariiqa gudbinta saxarada-afka, biyaha iyo cuntada wasakhaysan ee HEV ayaa si weyn u faafay, iyo halista infekshanka HEV ee dadka iyo xayawaanka ayaa ah mid sarreeya[1-2].
Kanaalka
FAM | HEV nucleic acid |
ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
Kaydinta | ≤-18℃ |
Nolosha shelf | 12 bilood |
Nooca Tuunada | Dhuunta dhuunta |
Tt | ≤38 |
CV | ≤5.0% |
LoD | 500 koobi/μL |
Gaar ahaan | Cagaarshowga E (HEV) waa fayras RNA ah oo keena dhibaatooyin caafimaad oo caalami ah.Waxay leedahay tiro badan oo martigeliyayaal ah waxayna leedahay hantida ka gudubta caqabadaha isbarbardhigga ah.Waa mid ka mid ah cudur-sidaha ugu muhiimsan ee zoonotic wuxuuna dhibaato weyn u geystaa dadka iyo duunyada.HEV inta badan waxaa lagu kala qaadaa gudbinta saxarada-afka, waxaana sidoo kale lagu kala qaadaa si toos ah embriyaha ama dhiiga.Waxaa ka mid ah, dariiqa gudbinta saxarada-afka, biyaha iyo cuntada wasakhaysan ee HEV ayaa si weyn u faafay, iyo halista infekshanka HEV ee dadka iyo xayawaanka ayaa ah mid sarreeya[1-2]. |
Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Codsaday Nidaamyada noolaha 7500 Nidaamka PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1
Qalabka Makro & Micro-Tijaabada Guud ee DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) HWTS-3006B).Waa in lagu soo saaraa si waafaqsan tilmaamaha.Mugga sifaynta lagu taliyey waa 80µL.
Doorashada 2
TIANAmp Virus DNA/RNA Kit (YDP315-R) oo ay samaysay Tiangen Biotech (Beijing) Co., Ltd. Waa in si adag loo soo saaro si waafaqsan tilmaamaha.Mugga saamiga la soo saaray waa 140 μL.Mugga caddaynta lagu taliyey waa 60µL.v