Qalabka Baadhista Aashitada Nucleic-ga ee Fayraska Ebola ee Bundybugyo (Fluorescence PCR)
Magaca alaabta
Qalabka Baadhista Aashitada Nucleic-ga ee Fayraska Ebola ee Bundybugyo (Fluorescence PCR)
Cudurrada Faafa
Filoviridae. Waa fayraska saddexaad ee Ebola ee sababi kara cudurro faafa oo aad u baaxad weyn oo aadanaha ah,ka dib markii uu ku dhacay cudurka ebola ee Zaire (EBOV) iyo cudurka ebola ee Sudan (SUDV). BDBV waa mid ka mid ahFayraska RNA-ga ee xudunta taban leh oo leh hiddo-side qiyaastii 19 kb, oo u dhigma 7qaab-dhismeedBorotiinno iyo 2 borotiin oo aan qaab-dhismeed lahayn. Muddada ku-meel-gaadhka ah ee caabuqa BDBV waa 2-21maalmo (celcelis ahaan 6.3 maalmood). Astaamihiisa aad bay ula mid yihiin kuwa fayrasyada kale ee Ebola.mana lagu kala saari karo astaamaha kiliinikada oo keliya. Bishii Agoosto 2007,faafitaanka qandhada dhiigbaxa fayraska ee aan la garanayn ayaa ka dhacay Degmada BundibugyoGalbeedka Uganda. Waxaa loo aqoonsaday inuu yahay fayras cusub oo Ebola ah waxaana loogu magac daray BundibugyoFayraska ebola (BDBV). Waxaa jiray 93 kiis oo looga shakisan yahay, 56 kiis oo la xaqiijiyay, iyo 37 dhimasho ah.(heerka dhimashada kiisaska 25%). Sannadkii 2012, cudur kale ayaa ka dhacay Gobolka Orientale eeJamhuuriyadda Dimuqraadiga ah ee Congo, iyadoo la xaqiijiyay 38 kiis iyo 13 dhimasho ah (kiis dhimasho ah)heerka 34%). Bishii Maajo 2026, WHO waxay ku dhawaaqday in cudurka BDBV uu hadda ka dilaacay dalkaJamhuuriyadda Dimuqraadiga ah ee Congo iyo Uganda oo ah Xaalad Degdeg ah oo Caafimaad DadweyneWalaaca Caalamiga ah (PHEIC). Ilaa Maajo 20, waxaa jiray 246 kiis oo looga shakisan yahay iyo 80 kiis oo kale.dhimasho.
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12bilo |
| Nooca Muunada | dhiig dhan, seeram/balasma,osuufka ral |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Nuqullo/mL |
| Qalabka Khuseeya | Nooca I:Nidaamka PCR-ga ee ABI 7500/7500 ee waqtiga-dhabta ah ee degdega ah, Nidaamka PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5, Nidaamka PCR-ga ee waqtiga-dhabta ah ee QuantStudio®7, Nidaamka Falanqaynta PCR-ga Caafimaadka ee Hongshi SLAN-96P/S oo si Buuxda Toos ah u shaqeeya, Nidaamka PCR-ga Waqtiga-Dhab ah ee LightCycler®480, Nidaamka PCR-ga ee waqtiga-dhabta ah ee Hangzhou Bioer FQD-96A, Nidaamka PCR-ga ee waqtiga-dhabta ah ee Yarui MA-6000, Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX96, Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96. Nooca II: Nidaamka Ogaanshaha Molekuleeriyada ee Tooska ah ee Eudemon AIO800 (HWTS-AIO800)
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Socodka shaqada:
Nooca I:
Nooca II:
Reagents-ka iskood isu diyaariya: Cusbada jirka, walxaha soo saara aashitada nukliyeer
Waxyaabaha la isticmaalo ee iskood isu diyaariya: Caarada tuubooyinka aan lahayn DNase/RNase, galoofisyo la tuuri karo, 1.5 mL
Tuubooyinka centrifuge-ka ee aan lahayn DNase/RNase, centrifuge-ka
