Fayraska Hargabka A Guud ahaan/H1/H3
Magaca alaabta
Qalabka Ogaanshaha Fayraska Influenza A ee HWTS-RT012 Universal/H1/H3 Nucleic Acid Multiplex (Fluorescence PCR)
Cudurrada Faafa
Fayraska hargabka waa nooc ka mid ah Orthomyxoviridae. Waa cudur faafa oo si xun u halis geliya caafimaadka aadanaha. Wuxuu si weyn u qaadi karaa hoyga. Cudurka faafa ee xilliyeedku wuxuu saameeyaa qiyaastii 600 milyan oo qof adduunka oo dhan wuxuuna sababaa 250,000 ~ 500,000 oo dhimasho ah, kuwaas oo fayraska hargabka A uu yahay sababta ugu weyn ee keenta caabuqa iyo dhimashada. Fayraska hargabka A waa RNA-ga hal-xadhig leh oo taban. Sida laga soo xigtay dusha sare ee hemagglutinin (HA) iyo neuraminidase (NA), HA waxaa loo qaybin karaa 16 nooc, NA Waxaa loo qaybiyaa 9 nooc. Fayrasyada hargabka A, noocyada hoose ee fayrasyada hargabka ee si toos ah u qaadi kara aadanaha waa: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 iyo H10N8. Kuwaas waxaa ka mid ah, noocyada hoose ee H1 iyo H3 waa kuwo aad u faafa, waxayna si gaar ah u mudan yihiin fiiro gaar ah.
Kanaal
| FAM | fayraska influenza A nooca caalamiga ah ee aashitada nukliyeerku |
| VIC/HEX | fayraska hargabka nooca H1 ee nooca hargabka A. Aashitada nukliyeerku |
| ROX | fayraska hargabka nooca H3 ee nooca hargabka A. Aashitada nukliyeerku |
| CY5 | xakamaynta gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | suufka sanka |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Nuqul/μL |
| Gaar ahaanshaha | Ma jiro falgal isku-dhafan oo lala sameeyo muunadaha neefsashada sida Hargabka A, Hargabka B, Legionella pneumophila, qandhada Rickettsia Q, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, iwm. iyo DNA-da genomic-ga aadanaha. |
| Qalabka Khuseeya | Nidaamka PCR-ga ee Waqtiga-dhabta ah ee La Adeegsaday ee Biosystems 7500 Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.) Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A, tignoolajiyada Hangzhou Bioer) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Soo saarista ama soo saarista Aashitada Nukliyeerka (YDP315-R) oo ay samaysay Tiangen Biotech (Beijing) Co., Ltd. Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 140μL, mugga lagu taliyay ee soo saarista waa 60μL.
Ikhtiyaarka 2aad.
Qalabka DNA/RNA ee Viral-ka ee Macro & Micro-Test (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 200μL, mugga dheecaanka lagu taliyayna waa 80μL.
