Fayraska Hargabka A Universal/H1/H3
Magaca alaabta
HWTS-RT012 Hargabka A Fayraska Universal/H1/H3 Qalabka lagu ogaado Acid Multiplex (Pluorescence PCR)
Epidemiology
Fayraska hargabku waa nooc wakiil ka ah Orthomyxoviridae. Waa cudur-sidaha cudur-sidaha oo khatar weyn u geysta caafimaadka aadanaha. Waxay si weyn u qaadsiin kartaa qofka guriga ku jira. Faafida xilliyeedku waxay saamaysaa qiyaastii 600 milyan oo qof oo adduunka oo dhan ah wuxuuna sababaa 250,000 ~ 500,000 dhimasho, kuwaas oo fayraska A-gu uu yahay sababta ugu weyn ee caabuqa iyo dhimashada. Hargabka A waa fayraska RNA-ku-xidhan taban. Marka loo eego dusha sare ee hemagglutinin (HA) iyo neuraminidase (NA), HA waxa loo qaybin karaa 16 nooc oo hoosaadyo ah, NA waxa loo qaybiyaa 9 nooc oo hoose. Fayrasyada hargabka A, noocyada hoosaadyada fayrasyada hargabka ee sida tooska ah u qaadi kara dadka waa: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 iyo H10N8. Waxaa ka mid ah, H1 iyo H3 nooc-hoosaadyadu aad bay u xanuunsadaan, waxayna si gaar ah u mudan yihiin fiiro gaar ah.
Kanaalka
| FAM | hargabka nooc caalami ah oo nucleic acid ah |
| VIC/HEX | hargabka A H1 nooca fayraska nucleic acid |
| ROX | hargabka A H3 nooca fayraska nucleic acid |
| CY5 | xakamaynta gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | naasopharyngeal suuf |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/μL |
| Gaar ahaan | Ma jiro falcelin ka hortag ah oo leh shaybaarada kale ee neefsashada sida Hargabka A, Hargabka B, Legionella pneumophila, Rickettsia Q qandhada, Chlamydia pneumoniae, Adenovirus, Fayraska syncytial ee Neefsashada, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Apiravirus/B1. Fayraska syncytial A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Fayraska Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, iwm iyo DNA genomic aadanaha. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista Ashitada Nukliyeerka ama Reagent Nadiifinta (YDP315-R) ee Tiangen Biotech (Beijing) Co., Ltd. Soo saarista waa in si adag loo fuliyaa si waafaqsan tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 140μL, mugga sareynta lagu taliyeyna waa 60μL.
Doorashada 2.
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Tijaabada Toomaatiga ah ee Acid Nucleic ah (HWTS-3006C, HWTS-3006B). Soo saarista waa in si adag loo fuliyaa si waafaqsan tilmaamaha isticmaalka. Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 80μL.
