Klebsiella Pneumoniae, Acinetobacter Baumannii iyo Pseudomonas Aeruginosa iyo hiddo-sidayaasha iska caabbinta daroogada (KPC, NDM, OXA48 iyo IMP) Multiplex

Klebsiella Pneumoniae, Acinetobacter Baumannii iyo Pseudomonas Aeruginosa iyo Hidde-sidayaasha iska caabbinta daroogada (KPC, NDM, OXA48 iyo IMP) Sawirka Muuqda ee Multiplex

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa in lagu ogaado tayada gudaha vitro ee Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) iyo afar hiddo-side oo iska caabiya carbapenem (oo ay ku jiraan KPC, NDM, OXA48 iyo IMP) oo ku jira muunadaha xaakada aadanaha, si loo bixiyo aasaaska hagitaanka baaritaanka caafimaad, daaweynta iyo daawada bukaanada qaba caabuqa bakteeriyada ee looga shakisan yahay.

Noo soo dir emayl

Faahfaahinta Badeecada

Calaamadaha Alaabta

Magaca alaabta

Qalabka Ogaanshada Multiplex ee HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii iyo Pseudomonas Aeruginosa iyo Hidde-sidayaasha iska caabbinta daroogada (KPC, NDM, OXA48 iyo IMP) (Fluorescence PCR)

Shahaadada

Cudurrada Faafa

Klebsiella pneumoniae waa cudur caadi ah oo ku dhaca unugyada maskaxda, waana mid ka mid ah bakteeriyada muhiimka ah ee keenta caabuqyada nosocomial. Marka iska caabbinta jirku yaraato, bakteeriyadu waxay ka gashaa sambabada marinnada neefsashada, taasoo keenta caabuq qaybo badan oo jirka ah, isticmaalka hore ee antibiyootikaduna waa furaha lagu daweeyo.^[1].

Goobta ugu badan ee caabuqa Acinetobacter baumannii waa sambabada, kaas oo ah cudur muhiim u ah oofwareenka Isbitaalka (HAP), gaar ahaan oofwareenka la xiriira hawo-mareenka (VAP). Badanaa waxaa la socda caabuqyo kale oo bakteeriya iyo fangaska ah, oo leh astaamo heer sare oo cudurro ah iyo heer dhimasho oo sarreeya.

Pseudomonas aeruginosa waa bacilli-ga aan khamiirin ee ugu badan ee ku jira dhaqanka caafimaadka, waana cudur-sidaha fursad-raadinta ee muhiimka ah ee caabuqa isbitaallada laga helo, oo leh astaamo gumeysi fudud, kala duwanaansho fudud iyo iska caabin daroogo badan leh.

Kanaal

Magaca	PCR-Mix 1	PCR-Mix 2
Kanaalka FAM	Aba	IMP
Kanaalka VIC/HEX	Xakamaynta Gudaha	KPC
Kanaalka CY5	PA	NDM
Kanaalka ROX	KPN	OXA48

Halbeegyada Farsamada

Kaydinta	≤-18℃
Muddada khaanadaha	12 bilood
Nooca Muunada	Xaako
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Gaar ahaanshaha	a) Tijaabada fal-celinta isdhaafka ah waxay muujinaysaa in qalabkani uusan lahayn fal-celin isdhaafsi ah oo lala sameeyo jeermisyada kale ee neefsashada, sida Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Neefsashada Adenovirus, Enterococcus iyo muunadaha xaakada oo aan lahayn bartilmaameedyo, iwm. b) Awoodda ka hortagga faragelinta: Xullo mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, iyo roxithromycin, iwm. si loogu sameeyo baaritaanka faragelinta, natiijooyinkuna waxay muujinayaan in walxaha faragelinta ee kor ku xusan aysan faragelin ku sameynin ogaanshaha Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa iyo hiddo-wadaha iska caabbinta carbapenem KPC, NDM, OXA48 iyo IMP.
Qalabka Khuseeya	Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio^®5 Nidaam oo PCR-Waqtiga-dhabta ah LightCycler^®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A, tiknoolajiyada Bioer) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96