Klebsiella Pneumoniae, Acinetobacter Baumannii iyo Pseudomonas Aeruginosa iyo Hiddeyaasha Iska caabinta Dawooyinka (KPC, NDM, OXA48 iyo IMP) Multiplex
Magaca alaabta
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii iyo Pseudomonas Aeruginosa iyo Hiddeyaasha Iska caabinta Dawooyinka (KPC, NDM, OXA48 iyo IMP) Qalabka Baahinta Multiplex (Fluorescence PCR)
Shahaado
CE
Epidemiology
Klebsiella pneumoniae waa cudur-fadeeye bukaan-socod oo caadi ah iyo mid ka mid ah bakteeriyada pathogenic ee muhiimka ah ee sababa caabuqyada hurdada. Marka iska caabbinta jidhku ay yaraato, bakteeriyadu waxay sambabada ka soo gashaa habka neefsashada, taasoo keenta caabuq ku dhaca qaybo badan oo jidhka ka mid ah, isticmaalka hore ee antibiyootiga ayaa ah furaha daaweynta.[1].
Meesha ugu badan ee caabuqa Acinetobacter baumannii waa sambabada, taas oo ah cudur keenaha muhiimka ah ee cusbitaalku helay oof wareenka (HAP), gaar ahaan Ventilator-ku xidhan oof wareenka (VAP). Badanaa waxaa weheliya caabuqyada kale ee bakteeriyada iyo fungal, oo leh astaamo heerka jirrooyinka sarreeya iyo heerka dhimashada sare.
Pseudomonas aeruginosa waa bacilli-ga ugu badan ee aan halsano ahayn ee garaam-negative bacilli ee ku-dhaqanka kiliinikada, waana cudur-fadeeye fursad muhiim u ah caabuqa isbitaalku helo, oo leh astaamo gumeysi fudud, kala duwanaansho fudud iyo iska caabbinta dawooyinka badan.
Kanaalka
| Magaca | PCR-Mix 1 | PCR-Mix 2 |
| FAM Channel | Aba | IMP |
| Kanaalka VIC/HEX | Xakamaynta Gudaha | KPC |
| Kanaalka CY5 | PA | NDM |
| Kanaalka ROX | KPN | OXA48 |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | xaako |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/ml |
| Gaar ahaan | a) Tijaabada falcelinta iskutallaabtu waxay muujinaysaa in qalabkani aanu lahayn falcelin iskutallaab ah oo leh cudur-sidaha kale ee neef-mareenka, sida Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemophilatic, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Adenovirus Neefsashada, Enterococcus iyo shaybaarrada xaakada oo aan lahayn bartilmaameed, iwm. b) Awoodda ka-hortagga faragelinta: Dooro mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, iyo roxithromycin, iwm. Acinetobacter baumannii, Pseudomonas aeruginosa iyo carbapenem hiddo-wadaha iska caabinta KPC, NDM, OXA48 iyo IMP. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, tignoolajiyada Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Wadarta Xalka PCR
