Fayraska Busbuska iyo Ku-qorista Acid Nucleic ah
Magaca alaabta
HWTS-OT202-Fayraska Busbuska iyo Qorida Xirmada Garashada Ashitada Nukliyeerka (Fluorescence PCR)
Shahaado
CE
Epidemiology
Busbuska (Mpox) waa cudur faafa oo ba'an oo zoonotic ah oo uu keeno Fayraska Monkeybux (MPXV). MPXV waa leben wareegsan ama qaab oval ah, waana fayraska DNA-da laba-geesooban oo dhererkiisu yahay 197Kb[1]. Cudurku inta badan waxaa gudbiya xayawaanka, dadkuna waxay ku qaadi karaan cudurka marka ay qaniinaan xayawaanka cudurka qaba ama taabashada tooska ah ee dhiigga, dareeraha jidhka iyo finan xayawaanka qaba. Fayrasku waxa kale oo lagu kala qaadi karaa dadka dhexdooda, ugu horrayn iyada oo loo marayo dhibcaha neefsashada marka ay dheeraato, waji ka waji toos ah ama taabashada tooska ah ee dareeraha jidhka bukaanka ama walxaha wasakhaysan.[2-3]. Daraasaduhu waxay muujiyeen in MPXV ay sameyso laba qaybood oo kala duwan: clade I (oo hore loogu yiqiin Central African clade ama Congo Basin clade) iyo clade II (hore loo yaqaan clade Galbeedka Afrika). Mpox of Congo Basin clade ayaa si cad loo muujiyay inuu u kala gudbi karo bini'aadamka wuxuuna sababi karaa dhimasho, halka suufka galbeedka Afrika uu keeno calaamado fudud oo uu leeyahay heer hoose oo isku gudbinta bini'aadamka iyo bini'aadamka.[4].
Natiijooyinka baaritaanka xirmadan looguma talagalin inay noqdaan calaamadda kaliya ee lagu ogaanayo caabuqa MPXV ee bukaannada, taas oo ay tahay in lagu daro astaamaha bukaan-socodka iyo xogta kale ee shaybaarka si loo saxo infekshanka bakteeriyada loona sameeyo qorshe daaweyn oo macquul ah si loo hubiyo badbaado iyo daaweyn waxtar leh.
Kanaalka
| FAM | MPXV clade II |
| ROX | MPXV universal nucleic acid |
| VIC/HEX | MPXV clade I |
| CY5 | xakamaynta gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | dheecaanka finan dadka, suufka oropharyngeal iyo serum |
| Ct | ≤38 (FAM, VIC/HEX, ROX), ≤35（IC) |
| LoD | 200 koobi/ml |
| Qalabka lagu dabaqi karo | Nooca 1 ee ogaanshaha reagent: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamyada ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah Nooca II reagent ogaanshaha: EudemonTMAIO800 (HWTS-EQ007)) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
