Fayraska Monkeypox iyo Nooca Nucleic Acid

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa in lagu ogaado tayada fayraska monkeypox clade I, clade II iyo fayraska monkeypox asiidhyada nucleic-ga ee dareeraha finanka aadanaha, suufka afka iyo muunadaha seeramka.

Noo soo dir emayl

Faahfaahinta Badeecada

Calaamadaha Alaabta

Magaca alaabta

Qalabka Ogaanshaha Aashitada Nukliyeerka ee Fayraska HWTS-OT202-Monkeypox iyo Nooca Fayraska Nukliyeerka (Fluorescence PCR)

Shahaadada

Cudurrada Faafa

Fayraska Monkeypox (Mpox) waa cudur faafa oo ku dhaca xayawaanka oo uu keeno Fayraska Monkeypox (MPXV). MPXV waa qaab wareegsan oo leben ah ama qaab u eg, waana fayras DNA ah oo laba-laaban oo dhererkiisu yahay qiyaastii 197Kb.^[1]Cudurkan waxaa inta badan gudbiya xayawaanka, dadkuna waxay ku dhici karaan qaniinyada xayawaanka qaba cudurka ama taabashada tooska ah ee dhiigga, dareeraha jirka iyo finanka xayawaanka qaba cudurka. Fayrasku waxa kale oo uu ku faafi karaa dadka dhexdooda, gaar ahaan dhibcaha neefsashada inta lagu jiro taabashada tooska ah ee fool-ka-fool ama taabashada tooska ah ee dareeraha jirka bukaanka ama walxaha wasakhaysan.^[2-3]Daraasaduhu waxay muujiyeen in MPXV ay samayso laba nooc oo kala duwan: clade I (oo hore loogu yiqiin clade-ka Bartamaha Afrika ama clade-ka Kongo) iyo clade II (oo hore loogu yiqiin clade-ka Galbeedka Afrika). spox-ka Congo Basin clade waxaa si cad loo muujiyay inuu isu gudbin karo aadanaha wuxuuna sababi karaa dhimasho, halka pox-ka Galbeedka Afrika uu keeno calaamado fudud wuxuuna leeyahay heer hoose oo ah gudbinta aadanaha iyo aadanaha.^[4].

Natiijooyinka baaritaanka ee xirmadan looguma talagalin inay noqdaan tilmaame keliya oo lagu ogaanayo caabuqa MPXV ee bukaanada, kaas oo ay tahay in lagu daro astaamaha caafimaad ee bukaanka iyo xogta kale ee baaritaanka shaybaarka si sax ah loogu qiimeeyo caabuqa cudurka iyo loo sameeyo qorshe daaweyn macquul ah si loo hubiyo daaweyn badbaado leh oo wax ku ool ah.

Kanaal

FAM	MPXV clade II
ROX	Aashitada nukliyeerka caalamiga ah ee MPXV
VIC/HEX	Qaybta MPXV I
CY5	xakamaynta gudaha

Halbeegyada Farsamada

Kaydinta	≤-18℃
Muddada khaanadaha	12 bilood
Nooca Muunada	dareere finan bini'aadam ah, suufka afka iyo seeramka
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35 (IC)
LoD	200 Nuqul/mL
Qalabka Khuseeya	Nooca I ee ogaanshaha falcelinta: Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio^®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler^®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A, Tiknoolajiyadda Hangzhou Bioer) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 Nooca II ee ogaanshaha sheyga: Eudemon^TMAIO800 (HWTS-EQ007)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.