Fayraska 'Monkepox' iyo qoritaanka aashitada nucloic acid

Fayraska 'Monkeypox virus' iyo qorida sawirka aashitada nucleic acid ee muuqaalka

Sharraxaadda Gaaban:

Qalabkan waxaa loo isticmaalaa ogaanshaha aqoonta vittro ee ku saabsan vittorica fayraska 'Monkepox fayras iI iyo daanyeerka fayraska fayraska finanka ee dheecaanka dareeraha ah ee dheecaanka bini-aadamka, obpophyngngeal iyo srab swars.

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HWTS-OT202-MONKKEPOX IYO CODSIGIIsa KITH OO KU SAABSAN XAALADAHA NUCLEEIC (PCR)

Shahaado

Nadiifin

MPKEPOX (MPXIX) waa cudur faafa si daran oo lagu faafiyo oo ay keento fayraska 'Monkepox virus (MPXV). MPXV waa mid dhameystiran ama qaab ama qaab qaab ah, waana virus 'oo laba laab ah oo dherer ah oo dherer ah qiyaastii 197kb^[1]. Cudurka waxaa badiyaa lagu kala qaadaa xayawaanka, oo bini-aadamkuna way ku dhici karaan inay qaniinto xayawaan cudurka qaba ama taabashada tooska ah ee dhiigga, dareeraha jirka iyo finanka xayawaanka cudurka qaba. Fayraska sidoo kale waxaa lagu kala qaadi karaa inta u dhexeysa dadka, ugu horrayn dhibco dhibco ah inta lagu gudajiro, wajiga wajiga ee daba dheeraada ama wajiga tooska ah ee lala yeesho dareeraha jidhka bukaanka ama walxaha wasakhaysan^[2-3]. Daraasaduhu waxay muujiyeen in MPXV uu sameeyo laba clardes oo kala duwan: Xadhiga I (oo loo yaqaan "Clads Central Clade ama Congo Barin Clade) iyo Clade II (oo horay loogu yeero Clade Galbeedka Afrika). MPOx ee Clade-ka Congo ee Congo ayaa si cad loo muujiyey in lagu kala qaado inta u dhexeysa bini-aadamka oo ay sababi karaan dhimasho, halka MPOx ee xiidmaha ee Galbeedka Afrika ay keenaan astaamo khafiif ah waxayna leeyihiin sicir-miradka bini-aadamka^[4].

Natiijooyinka tijaabada ah ee loogu talagalay in lagu muujiyo tusaha kaliya ee cudurka infakshanka MPXV ee bukaanka, oo ay tahay in lagu daro astaamaha bukaan-socodka bukaanka iyo xogta kale ee tijaabada shaybaarka si loo garsooro infakshanka cudurka loo yaqaan 'patters' iyo sameynta daaweyn macquul ah Qorsheyso inaad hubiso daaweyn aamin ah oo wax ku ool ah.

Kanaal

Fa farqad	Mpxv clade II
Rox	MPXV Universal Universal Acid Nucleic
Vic / hex	Mpxv conde ide i
Cy5	kontoroolka gudaha

Cayayaanka farsamada

Bakhaar	≤-18 ℃
Shelf-Life	12 bilood
Nooca muunadda	dheecaanka bini-aadamka, xijaabka oropharygeal iyo serum
Ct	≤38 (FAC, Vic / Hex, rox), ≤35 (IC)
Dhigid	200 nuqul / ml
Aaladaha lagu dabaqi karo	Nooca aan ogaado dib-u-soo-celinta: Waxaa lagu dabaqay biosystemy-ga ee lagu dabaqay 7500 nidaamyada waqtiga-dhabta ah ee PCR Waxaa lagu dabaqay bio-yariyada bio-yada lagu dabaqay 7500 oo si dhakhso leh u ah nidaamyada PCR-ga TiarStudio^®5 Nidaamyada PCR-ka waqtiga-dhabta ah Nidaamyada Slan-96p ee waqtiga-labaad ee PCR (tikniyoolajiyadda caafimaadka ee Hongshi ee Co., Ltd.) Nalalka^®480 Nidaamka PCR-ka dhabta ah Sadarka 9600 oo lagu daray nidaamyada ogaanshaha PCR-ga ee waqtiga-labaad (FQD-96A, tikniyoolajiyadda biologjiilaynta bioer) MA-6000 DIIWAAN GELINTA EE MADAXWEYNAHA (SUZHOU MolARYADA CODKA, LTD.) Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Biorad Cfx Opus 96 Nidaamka PCR-ka waqtiga-dhabta ah Nooca II Ogeysiinta Reaggent: Eudemon^TMAio800 (hwts-eq007) Waxaa qoray Jiangsu Macro & Micro-Tijaabada Med-Tech Co., Ltd.