MTHFR Gene Polymorphic Acid Nucleic
Magaca alaabta
HWTS-GE004-MTHFR Qalabka Garashada Ashitada Nukliyeerka ee Gene Polymorphic (ARMS-PCR)
Epidemiology
Folic acid waa fiitamiin biyo-milmi kara kaas oo ah qaybiye lagama maarmaan u ah waddooyinka dheef-shiid kiimikaadka ee jirka.Sanadihii la soo dhaafay, tiro badan oo daraasado ah ayaa xaqiijiyay in, isbeddelka folate metabolizing hidda-wadaha MTHFR ay horseedi doonto yaraanta folic acid ee jirka, iyo dhaawaca guud ee yaraanta folic acid ee dadka qaangaarka ah waxay keeni kartaa megaloblastic anemia, xididdada xididdada dhiigga. Dhaawaca endothelial, iwm. Folic acid yaraanta haweenka uurka leh ma dabooli karto baahida naftooda iyo uurjiifka, taas oo keeni karta cilladaha tuubada neerfaha, anencephaly, bakhti iyo dhicin.Heerarka folate-ka serum waxaa saameeya 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Isbeddellada 677C>T iyo 1298A>C ee hiddo-wadaha MTHFR waxay keenaan beddelka alanine ee valine iyo glutamic acid, siday u kala horreeyaan, taasoo keentay hoos u dhaca dhaqdhaqaaqa MTHFR oo sidaas awgeed hoos u dhac ku yimid isticmaalka folic acid.
Kanaalka
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC(HEX) | Xakamaynta gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Dhiig cusub oo EDTA laga soo ururiyey |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Qalabka lagu dabaqi karo: | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah SLAN ®-96P Nidaamyada PCR-waqtiga-dhabta ah QuantStudio™ 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1
Soosaarayaasha lagu taliyay: Macro & Qalabka DNA-ga ee Micro-Tijaabada Genomic (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) .
Doorashada 2
Soo saarista lagu taliyay: Qalabka Soo saarista DNA-da Dhiiga Genomic (YDP348, JCXB20210062) ee Tiangen Biotech(Beijing) Co., Ltd.Qalabka Soosaarka Genome-ka Dhiiga (A1120) ee Promega.
