Aashitada Nukliyeerka ee Polymorphic-ka ee Hiddaha MTHFR
Magaca alaabta
Qalabka Ogaanshada Aashitada Nukliyeerka ee HWTS-GE004-MTHFR ee Hidde-sideyaasha Polymorphic Nukliyeerka (ARMS-PCR)
Cudurrada Faafa
Folic acid waa fiitamiin milma biyaha kaas oo ah qayb muhiim ah oo ka mid ah hababka dheef-shiid kiimikaadka jirka. Sannadihii ugu dambeeyay, tiro badan oo daraasado ah ayaa xaqiijiyay in isbeddelka hidda-wadaha enzyme-ka ee folate-ka MTHFR uu horseedi doono yaraanta folic acid ee jirka, dhaawaca guud ee yaraanta folic acid ee dadka waaweynna uu sababi karo dhiig-yaraan megaloblastic ah, dhaawac ku yimaada endothelial-ka xididdada dhiigga, iwm. Yaraanta folic acid ee haweenka uurka leh ma dabooli karto baahiyaha naftooda iyo uurjiifka, taasoo keeni karta cillado tuubo neerfaha ah, anencephaly, ilmo-dhalasho, iyo dhicis. Heerarka folate-ka ee serum-ka waxaa saameeya isbeddellada 5,10-methylenetetrahydrofolate reductase (MTHFR). Isbeddellada 677C>T iyo 1298A>C ee hidda-wadaha MTHFR waxay kiciyaan beddelka alanine una beddelo valine iyo glutamic acid, siday u kala horreeyaan, taasoo keentay hoos u dhac ku yimaada dhaqdhaqaaqa MTHFR iyo sidaas darteedna hoos u dhaca isticmaalka folic acid.
Kanaal
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC (HEX) | Xakamaynta gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Dhiig cusub oo laga sameeyay EDTA oo xinjirowga lidka ku ah |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Qalabka Khuseeya: | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee SLAN ®-96P Nidaamyada PCR-ga ee QuantStudio™ 5-ta Waqti-dhabta ah Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaar 1
Waxyaabaha lagu taliyay ee soo saarista: Qalabka DNA-da ee Macro & Micro-Test (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Ikhtiyaarka 2aad
Waxyaabaha lagu taliyay ee soo saarista DNA-da ee dhiigga (YDP348, JCXB20210062) oo ay samaysay Tiangen Biotech(Beijing) Co.,Ltd. Qalabka Soo saarista Hidde-sidayaasha Dhiigga (A1120) oo ay samaysay Promega.
