Mycoplasma Pneumoniae (MP)
Magaca alaabta
HWTS-RT024 Mycoplasma Pneumoniae (MP) Qalabka Ogaanshaha Asiidhka Nucleic (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) waa nooc ka mid ah microorganism-ka ugu yar ee prokaryotic, kaas oo u dhexeeya bakteeriyada iyo fayraska, oo leh qaab dhismeed unug laakiin aan lahayn derbi unug. MP ayaa inta badan sababa caabuqa habka neef-mareenka ee bini'aadmiga, gaar ahaan carruurta iyo dhalinyarada. Waxay keeni kartaa oof wareenka mycoplasma bani'aadamka, caabuqa mareenka neefsiga ee carruurta iyo oof-wareen aan caadi ahayn. Calaamadaha caafimaadku waa kala duwan yihiin, kuwaas oo intooda badan ay yihiin qufac daran, qandho, qarqaryo, madax-xanuun, cune xanuun. Caabuqa hawada sare ee neef-mareenka iyo sambabada bronki ayaa ah kuwa ugu badan. Bukaanjiifka qaarkood waxay ka qaadi karaan caabuqa neef-mareenka sare ilaa oof wareen daran, cidhiidhi neefsi oo daran iyo dhimasho ayaa dhici karta.
Kanaalka
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Xaaka, suufka afka Orpharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 koobi/ml |
| Gaar ahaan | a) falcelin iskutallaab ah: ma jiro falcelin iskutallaab ah oo leh Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Hargabka Haemophilus, Hargabka Haemophilus, Klebsiella pneumoniae, Staphylococcus, qaaxada Mycoccus aureone. pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Fayraska Hargabka A, Fayraska Hargabka B, fayraska Parainfluenza nooca I/II/III/IV, Rhinovirus, Adenovirus, Metapneumovirus aadanaha, Fayraska syncytial ee neefsiga iyo genomic nucleic acid aadanaha. b)Awoodda ka hortagga faragelinta: ma jirto wax faragelin ah marka walxaha faragelinta lagu tijaabiyo xajmiyada soo socda: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) dhiiga dadka, levofloxacin (10μg/mL), moxifloxacin(0μg/L), moxifloxacin (80μg/ml), azithromycin (1mg/mL), clarithromycin (125μg/ml), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
(1) Tusaalaha xaakada
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 200µL oo cusbo ah oo caadi ah roobka la warshadeeyay. Soo saarista ku xigta waa in la sameeyaa si waafaqsan tilmaamaha isticmaalka. Mugga sare-u-qaadista ee lagu taliyey waa 80µL. Soo saarista lagu taliyey: Soosaarka Asiidhka Nukliyeerka ama Reagent Nadiifinta (YDP315-R). Soo saarista waa in si adag loo sameeyaa si waafaqsan tilmaamaha isticmaalka. Mugga sifaynta lagu taliyey waa 60µL.
(2) suufka Oropharyngeal
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in loo sameeyaa si waafaqsan tilmaamaha isticmaalka. Mugga soo saarista lagu taliyey ee muunadda waa 200µL, mugga elutionkana waa 80µL. Soo saarista lagu taliyey: QIAamp Viral RNA Mini Kit (52904) Soo saarista waa in si adag loo sameeyaa si waafaqsan tilmaamaha isticmaalka. Mugga soo saarista lagu taliyey ee muunadda waa 140µL, mugga sareynta lagu taliyeyna waa 60µL.
