Tijaabada EasyAmp ee Macro & Micro—- Qalab Kor u Qaadis Iftiiminta Iftiiminta oo la qaadan karo oo la jaan qaadi kara LAMP/RPA/NASBA/HDA

Waxqabadka Wanaagsan & Codsiga Ballaaran

Easy Amp, iyadoo la adeegsanayo tiknoolajiyada iKordhinta aashitada nukliyeer-ka ee sothermalwaxaa lagu soo bandhigay qaab sarexasaasiyad iyogaabanfalcelinmuddo aan lahayn shuruudo habka beddelka heerkulka. Sidaa darteed, waxay leedahaysoo baxedsidainta badanTiknoolajiyadda la doorbiday ee tijaabada degdega ah ee aashitada nukliyeer-ka.Iyada oo aan lahayn t adagNidaamyada wareegga dhirta, qalab fudud oo la qaadi karo oo kor u qaadaya kulaylka isothermalqancin karahabka falcelinta oo dhan.

Macro & Micro-Test si madax-bannaan ayaa loo sameeyayoo la bilaabayEasyAmp,qalab kor u qaadis ah oo la qaadi karo oo ah qalabka kor u qaadista kulaylka ee fluorescent-ka ahcodsiyada kala duwan.

Waqtigan xaadirka ah,Amp Easy waxaa lagu xaqiijiyay inay tahayla jaan qaadaya dhowr farsamooyin kor u qaadis ah oo isothermal ah: Kor u qaadis Isothermal ah oo Loop-Mediated (LAMP), Kor u qaadis Polymerase Recombinase (RPA/RAA/MIRA), Kor u qaadis ku salaysan Nucleic Acid (NASBA), iyo Kor u qaadis ku tiirsan Helicase (HDA)Waa qalab kale oo casri ah oo loogu talagalay baaritaanka aashitada nukliyeer-ka ee degdega ah iyadoo la adeegsanayo barnaamijyo aad u ballaaran.

· Kor-u-qaadista Isothermal-ka ee Wareegga-dhexdhexaadka ah (LAMP):Ballaarinta at60-65°Coo dhanfalcelinhabkala dhammeeyay 30 daqiiqo gudahood.Markaan fiirinayo Jaantuska 1aad ee kor u qaadistamabda'a:

 

Jaantuska 1aad. Mabda'aof LAAMKA 

· Dib-u-isku-darka Polymerase Amplification (RPA/RAA/MIRA):Ballaarinta at37°C-42°Coo dhanfalcelinhabkawaxaa lagu dhammeeyay 10-30 daqiiqo gudahood.Markaan fiirino Jaantuska 2aad ee ku saabsanmabda'a ballaarinta:

Jaantuska 2aad. Mabda'aof Kordhinta Polymerase-ka ee Dib-u-isku-darka

·Kordhinta Taxanaha Aashitada Nukliyeerka (NASBA):Ballaarinta at42°Coo dhanfalcelinhabkawaxaa la dhammeeyaa 40-120 daqiiqo gudahood.Eeg Jaantuska 3aad si aad u aragtomabda'a ballaarinta:

Jaantuska 3aad. Mabda'aof Kordhinta Taxanaha Aashitada Nukliyeerka

· Awood-siinta Ku-tiirsanaanta Helicase (HDA):Ballaarinta at65°Coo dhanfalcelinhabkawaxaa la dhammeeyaa 70-90 daqiiqo gudahood.Eeg Jaantuska 4aad si aad u aragtomabda'a ballaarinta:

Jaantuska 4aad. Mabda'aof Kordhinta Ku-tiirsanaanta Helicase

· EasyAmpawood u yeeloKordhinta waxtarka leh iyo xasilloonida ee ogaanshaha DNA-da iyo RNA iyadoo la adeegsanayo afarta farsamo ee isothermal ee kor ku xusan. Waxay gaartaa xasaasiyadda kordhinta ilaa 200 nuqul/mL waxayna hubisaa gaar ahaansho sare iyada oo aan lahayn falcelin isdhaafsi ah oo ku saabsan kordhinta bartilmaameedka.(Ilagu sawiray Jaantusyada 5 ilaa 7).

Ogaanshaha DNA-da Ogaanshaha RNA-da

Jaantuska 5aad. Madal RAA/MIRA

Ogaanshaha DNA-da Ogaanshaha RNA-da

Jaantuska 6aad. Madal LAMP

 

Ogaanshaha DNA-da Ogaanshaha RNA-da

Jaantuska 7aad. Madal NASBA

Faa'iidooyinka Badeecada:

Degdeg ah

· EPIAtiknoolajiyadu waxay ogaataynatiijo toganlagu ogaaday 5 daqiiqo gudahood.

· Hawlgal fudud oo leh soo saarid hal-tallaabo ahsi loogu kordhiyo qalabka.

Wax ku ool ah

· 4 qaybood oo madax-bannaan oo loogu talagalay marka la dalbado, tijaabin isku mar ah oo 4 nooc oo kala duwan ahnooca tijaabadas.

· Qayb kasta oo awood u leh inay falanqeyso afar muunad isku mar.

· Laba/Afarfluorescencekanaallosi loo helo ogaanshaha bartilmaameedyo badan oo ballaaran.

Iswaafajinta

· La jaan qaadi karabadanGoobaha kuleylka ee isothermal-ka sida LAMP, RPA, NASBA, HDA.

· Loogu talagalay kor u qaadista heerkulka sare ee qoraalka dib-u-celinta heerkulka hooseeya kadib.

· Habaynawood u lehsi loo daboolo baahiyaha gaarka ah ee macaamiisha.

Isticmaale-saaxiibtinimo leh

· Pnaqshad la qaadi karo(290×245×128mm, 2kg) loogu talagalay codsiyada kala duwan ee waayeelka.

· Shaashadda taabashada midabka 7-inji ahsi loo fududeeyo hawlgalka iyo sawir natiijo cad leh.

· Daabacaha kuleylka ee ku dhex jira si loogu daabaco natiijooyinka degdega ah.

· Iskaan isku dhafan oo loogu talagalay akhrinta shay si otomaatig ah loo ogaado.