Cudurada Neefsashada ee La isku daray

Sharaxaad Gaaban:

Xirmadaan waxaa loo isticmaalaa in vitro lagu ogaado tayada fayraska hargabka A, fayraska hargabka B, fayraska syncytial neefta, adenovirus, rhinovirus aadanaha iyo mycoplasma pneumoniae nucleic acids ee sanka sanka bini'aadamka iyo muunadaha suufka oropharyngeal.Natiijooyinka baaritaanka waxaa loo isticmaali karaa kaalmaynta ogaanshaha caabuqa caabuqa neef-mareenka, iyo in la siiyo saldhig ogaanshaha molecular kaaliyaha ah ee ogaanshaha iyo daawaynta caabuqa caabuqa neef-mareenka.

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HWTS-RT050-Lix Nooc oo ah Qalabka Lagu Ogaado Ashitada Nucleic-ka ee Neefsashada(Fluorescence PCR)

Epidemiology

Hargabka, oo loo yaqaan 'flu', waa cudur faafa oo neef-mareenka oo ba'an oo uu keeno fayraska hargabka, kaas oo aad u faafa oo inta badan lagu kala qaado qufaca iyo hindhisada.

Fayraska isku dhafan ee neefsashada (RSV) waa fayras RNA, oo ka tirsan qoyska paramyxoviridae.

Adenovirus (HAdV) waa fayras DNA oo labanlaaban oo aan lahayn baqshad.Ugu yaraan 90 genotypes ayaa la helay, kuwaas oo loo qaybin karo 7 subgenera AG.

Human rhinovirus (HRV) waa xubin ka mid ah qoyska Picornaviridae iyo genus Enterovirus.

Mycoplasma pneumoniae (MP) waa microorganism pathogenic kaas oo u dhexeeya bakteeriyada iyo fayraska xajmiga.

Kanaalka

Kanaalka	PCR-Mix A	PCR-Mix B
FAM Channel	IFV A	HADV
Kanaalka VIC/HEX	HRV	IFV B
Kanaalka CY5	RSV	MP
Kanaalka ROX	Xakamaynta Gudaha	Xakamaynta Gudaha

Qiyaasaha Farsamada

Kaydinta	-18℃
Nolosha shelf	12 bilood
Nooca Tuunada	Oropharyngeal suuf
Ct	≤35
LoD	500 koobi/ml
Gaar ahaan	1.Natiijooyinka tijaabada falcelinta falcelinta waxay muujisay inaysan jirin falcelin iskutallaab ah oo udhaxeysa xirmada iyo coronavirus aadanaha SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, noocyada fayraska Parainfluenza 1, 2, iyo 3, Chlamydia pneumoniae, metapneumovirus aadanaha, Enterovirus A, B, C, D, Fayraska Epstein-Barr, Fayraska jadeecada, cytomegalovirus aadanaha, Rotavirus, Norovirus, Fayraska qaamo-qashiir, fayraska Varicella-zoster, Legionella, Bordetella pertussis, Haemophilus influenkozae. aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, qaaxada Mycobacterium, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans iyo aadanaha genomic nucleic acids. 2.Awoodda ka-hortagga faragelinta: Mucin (60mg / mL), 10% (v / v) dhiigga bini'aadamka, phenylephrine (2mg / mL), oxymetazoline (2mg / mL), chloride sodium (oo leh walxaha ilaaliya) (20mg / mL), beclomethasone ( 20mg/ml), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/ml), mometasone (2mg/ml), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alfa-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/ml), peramivir (1mg/mL), lopinavir (500mg/ml), Ritonavir (60mg/ml), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/ml), Meropenem (200mg/ml), levofloxacin (10μg/ml), iyo tobramycin (0.6mg/mL) ayaa loo xushay baaritaanka faragelinta, natiijaduna waxay muujisay in walxaha faragelinta ee uruurinta kor ku xusan aysan lahayn fal-celin faragelin ah oo ku saabsan natiijooyinka baaritaanka cudur-sidaha.
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