Cudurada Neefsashada ee La isku daray
Magaca alaabta
HWTS-RT183-Xabadaha ogaanshaha Isku-darka ah ee Neefsashada (Fluorescence PCR)
Epidemiology
Cudurka Corona Virus 2019, oo loo yaqaan 'COVID-19', waxaa loola jeedaa oof wareenka uu keeno caabuqa SARS-CoV-2. SARS-CoV-2 waa coronavirus ka tirsan β-genus. COVID-19 waa cudur faafa oo neef-mareenka oo ba'an, dadkuna guud ahaan waa u nugul yihiin. Waqtigan xaadirka ah, isha infekshanku waa inta badan bukaanada uu haleelay 2019-nCoV, iyo dadka asymptomatic-ka qaba ayaa sidoo kale noqon kara isha caabuqa. Iyada oo ku saleysan baaritaanka cudurrada faafa ee hadda, muddada soo-kabashada waa 1-14 maalmood, badiyaa 3-7 maalmood. Qandho, qufac qallalan iyo daal ayaa ah calaamadaha ugu waaweyn. Bukaanno dhowr ah ayaa lahaa astaamo ay ka mid yihiin ciriiri sanka ah, sanka oo duufsan, cune xanuun, myalgia iyo shuban, iwm. Hargabka, oo loo yaqaan 'flu', waa cudur faafa oo neef-mareenka oo ba'an oo uu keeno fayraska hargabka. Aad buu u faafayaa. Inta badan waxa lagu kala qaadaa qufaca iyo hindhisada. Caadiyan waxay soo baxdaa guga iyo jiilaalka. Fayrasyada hargabku waxay u qaybsan yihiin hargab A (IFV A), hargabka B (IFV B), iyo Hargabka C (IFV C) saddex nooc, dhammaan waxay ka tirsan yihiin fayras dhegdheg ah, waxay sababaan cudurrada bini'aadamka inta badan hargabka A iyo fayrasyada B, waa fayraska RNA oo hal-ganacsan. Hargabka A waa caabuq neef-mareenka oo degdeg ah, oo ay ku jiraan H1N1, H3N2 iyo noocyo kale oo hoose, kuwaas oo u nugul isbeddelka iyo dillaaca adduunka oo dhan. 'Shift' waxa loola jeedaa beddelka fayraska hargabka A, taasoo keentay soo bixitaanka fayras 'subtype' cusub. Fayrasyada hargabka B waxa ay u kala qaybsan yihiin laba abtirsiimo, Yamagata iyo Victoria Hargabka B fayraska kaliya waxa uu leeyahay fayraska antigenic, waxana uu ka baxsadaa ilaalinta iyo ciribtirka habka difaaca bini'aadamka iyada oo loo marayo isbeddelkiisa. Si kastaba ha ahaatee, xawaaraha kobaca ee fayraska hargabka B wuu ka gaabiyaa kan fayraska hargabka A. Fayraska hargabka B wuxuu kaloo keeni karaa caabuqyada neef-mareenka ee bani'aadamka wuxuuna u horseedi karaa cudurro faafa.
Fayraska isku dhafan ee neefsashada (RSV) waa fayras RNA, oo ka tirsan qoyska paramyxoviridae. Waxaa lagu kala qaadaa dhibco hawo ah iyo xiriir dhow waana cudur keenaha ugu weyn ee caabuqa mareenka neef-mareenka hoose ee dhallaanka. Dhallaanka uu ku dhacay RSV waxaa laga yaabaa inay yeeshaan bronchiolitis daran iyo oof-wareen, kuwaas oo la xiriira neefta carruurta. Dhallaanku waxay leeyihiin calaamado aad u daran, oo ay ku jiraan qandho sare, rhinitis, pharyngitis iyo laryngitis, ka dibna bronkiolitis iyo oof-wareen. In yar oo carruur ah oo buka waxay ku adkaan karaan otitis media, pleurisy iyo myocarditis, iwm. Infekshanka mareenka sare ee neef-mareenka waa calaamadda ugu weyn ee caabuqa ee dadka waaweyn iyo carruurta ka weyn.
Qiyaasaha Farsamada
| Kaydinta | -18 ℃ Madow |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Oropharyngeal suuf; Nasopharyngeal suuf |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 koobi/μL |
| Gaar ahaan | Natiijooyinka falcelinta iskutallaabtu waxay muujinayaan in aysan jirin falcelin iskutallaab ah oo u dhexeeya xirmada iyo cytomegalovirus, fayraska herpes simplex nooca 1, fayraska varicella zoster, fayraska Epstein-Barr, adenovirus, metapneumovirus aadanaha, rhinovirus, fayraska parainfluenza nooca I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma p. Bordetella xiiq-dheer, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, noocyada la yareeyey ee qaaxada Mycobacterium, Neisseria meningitidis, Neisseria spp. Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcesbagilter. fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q qandho iyo genomic nucleic acid. |
| Qalabka lagu dabaqi karo | Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah, Hababka noolaha ee la dabaqay®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah, LineGene 9600 Plus-Time-Time PCR Systems Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX96 Real-Time PCR System, |
Socodka Shaqada
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (kaaso loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), iyo Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8)TM AIO800 (HWTS-EQ007)) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Mugga muunadda la soo saaray waa 200μL iyo mugga sareynta lagu taliyey waa 150μL.
