Lix nooc oo ah jeermisyada neefsashada

Lix nooc oo cudur-sidaha neefsashada ah ah Sawirka La Soo Bandhigay

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaali karaa in si tayo leh loogu ogaado aashitada nucleic-ga ee SARS-CoV-2, fayraska hargabka A, fayraska hargabka B, adenovirus, mycoplasma pneumoniae iyo fayraska isku-dhafka neefsashada ee in vitro.

Noo soo dir emayl

Faahfaahinta Badeecada

Calaamadaha Alaabta

Magaca alaabta

Qalabka HWTS-OT058A/B/C/Z-Qalabka RT-PCR ee waqtiga-dhabta ah ee fluorescent-ka ah si loo ogaado lix nooc oo cudurro neef-mareenka ah

Shahaadada

Cudurrada Faafa

Cudurka Fayraska Corona 2019, oo loo yaqaan "COVID-19", waxaa loola jeedaa oof-wareenka uu keeno caabuqa SARS-CoV-2. SARS-CoV-2 waa fayras ka tirsan β genus. COVID-19 waa cudur faafa oo neef-mareenka ah, dadkuna guud ahaan way u nugul yihiin. Waqtigan xaadirka ah, isha caabuqa waxaa ugu badan bukaanada uu ku dhacay SARS-CoV-2, dadka qaba cudurka aan calaamadaha lahayn ayaa sidoo kale noqon kara isha caabuqa. Iyada oo lagu saleynayo baaritaanka cudurka faafa ee hadda socda, muddada cudurka waa 1-14 maalmood, inta badan 3-7 maalmood. Qandho, qufac qalalan iyo daal ayaa ah astaamaha ugu muhiimsan. Dhowr bukaan ayaa qabay sanka oo xirma, sanka oo dareere ah, cuna xanuun, myalgia iyo shuban.

Hargabka, oo loo yaqaan "hargabka", waa cudur neef-mareenka ah oo degdeg ah oo uu keeno fayraska hargabka. Aad ayuu u faafaa. Waxaa badanaa lagu kala qaadaa qufaca iyo hindhisada. Badanaa wuxuu dilaacaa guga iyo jiilaalka. Fayrasyada hargabka waxaa loo qaybiyaa hargabka A, IFV A, hargabka B, IFV B, iyo hargabka C, IFV C saddex nooc, dhammaantood waxay ka tirsan yihiin fayras dhegdheg leh, waxayna keenaan cudurrada aadanaha inta badan fayrasyada hargabka A iyo B, waa fayras RNA oo hal-xadhig leh, oo kala qaybsan. Fayraska Hargabka A waa caabuq neef-mareenka oo degdeg ah, oo ay ku jiraan H1N1, H3N2 iyo noocyo kale, kuwaas oo u nugul isbeddelka iyo faafitaanka adduunka oo dhan. "Shift" waxaa loola jeedaa isbeddelka fayraska hargabka A, taasoo keenta soo ifbaxa "nooc hoose" oo fayras cusub ah. Fayrasyada Hargabka B waxaa loo qaybiyaa laba nooc, Yamagata iyo Victoria. Fayraska Hargabka B wuxuu leeyahay oo keliya isbeddel antigenic ah, wuxuuna ka baxsadaa la socodka iyo baabi'inta nidaamka difaaca jirka aadanaha iyada oo loo marayo isbeddelkiisa. Si kastaba ha ahaatee, xawaaraha isbeddelka fayraska hargabka B waa ka gaabis badan kan fayraska hargabka A ee aadanaha. Fayraska hargabka nooca B wuxuu sidoo kale sababi karaa caabuqyada neefsashada ee aadanaha wuxuuna horseedi karaa cudurro faafa.

Fayraska Adenovirus (AdV) wuxuu ka tirsan yahay naasleyda adenovirus, kaas oo ah fayras DNA ah oo labanlaab ah oo aan lahayn baqshad. Ugu yaraan 90 nooc oo hidde ah ayaa la helay, kuwaas oo loo qaybin karo noocyada hoose ee AG 7. Caabuqa AdV wuxuu sababi karaa cuduro kala duwan, oo ay ku jiraan oofwareen, boronkiito, cystitis, indhaha oo ku dhaca, cudurrada caloosha iyo mindhicirka iyo encephalitis. Oofwareenka Adenovirus waa mid ka mid ah noocyada ugu daran ee oofwareenka ay bulshadu qaaddo ee carruurta, taasoo ka dhigan qiyaastii 4%-10% oofwareenka ay bulshadu qaaddo.

Mycoplasma pneumoniae (MP) waa nooc ka mid ah noolaha ugu yar ee prokaryotic, kaas oo u dhexeeya bakteeriyada iyo fayraska, oo leh qaab-dhismeedka unugyada laakiin aan lahayn derbi unug. MP waxay inta badan keentaa caabuq ku dhaca marinnada neefsashada ee aadanaha, gaar ahaan carruurta iyo dhallinyarada. Waxay sababi kartaa oof-wareenka mycoplasma ee aadanaha, caabuqa marinnada neefsashada ee carruurta iyo oof-wareen aan caadi ahayn. Calaamadaha caafimaadku way kala duwan yihiin, kuwaas oo intooda badan yihiin qufac daran, qandho, qarqaryo, madax-xanuun, cuna xanuun. Caabuqa marinnada neefsashada sare iyo oof-wareenka bronki ayaa ah kuwa ugu badan. Bukaannada qaar waxay ku dhici karaan caabuqa marinnada neefsashada sare ilaa oof-wareen daran, dhibaato neefsasho oo daran iyo dhimasho ayaa dhici karta.

Fayraska isku-dhafka neef-mareenka (RSV) waa fayras RNA ah, oo ka tirsan qoyska paramyxoviridae. Waxaa lagu kala qaadaa dhibcaha hawada iyo xiriirka dhow waana cudur-sidaha ugu weyn ee keena caabuqa marinnada neef-mareenka ee hoose ee dhallaanka. Dhallaanka qaba cudurka RSV waxay yeelan karaan boronkiito daran (oo loo yaqaan bronchiolitis) iyo oof-wareen, kuwaas oo la xiriira neefta carruurta. Dhallaanka waxay leeyihiin calaamado daran, oo ay ku jiraan qandho sare, rhinitis, pharyngitis iyo laryngitis, ka dibna bronchiolitis iyo oof-wareen. Carruur yar oo xanuunsan ayaa ku adkaan kara otitis media, pleurisy iyo myocarditis, iwm. Caabuqa marinnada neef-mareenka sare waa calaamadda ugu weyn ee caabuqa dadka waaweyn iyo carruurta waaweyn.

Kanaal

Magaca kanaalka	Kaydka Falcelinta R6 A	Kaydka Falcelinta R6 B
FAM	SARS-CoV-2	HAdV
VIC/HEX	Xakamaynta Gudaha	Xakamaynta Gudaha
CY5	IFV A	MP
ROX	IFV B	RSV

Halbeegyada Farsamada

Kaydinta	Dareere: ≤-18℃ Mugdi; Lyophilized: ≤30℃ Mugdi
Muddada khaanadaha	Dareere: 9 bilood; La daaray: 12 bilood
Nooca Muunada	Dhiig dhan, Balaasma, Serum
Ct	≤38
CV	≤5.0％
LoD	300 Nuqullo/mL
Gaar ahaanshaha	Natiijooyinka fal-celinta isdhaafka ah waxay muujiyeen in aysan jirin wax falcelin isdhaaf ah oo u dhexeeya qalabka iyo fayraska fayraska SARSr-CoV ee aadanaha, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, nooca fayraska parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, aadanaha metapneumovirus, enterovirus A, B, C, D, fayraska sambabada aadanaha, fayraska epstein-barr, fayraska jadeecada, fayraska cytomegalo ee aadanaha, rotavirus, norovirus, fayraska parotitis, fayraska varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium TB, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci iyo cryptococcus cusub iyo aashitada genomic genomic ee aadanaha.
Qalabka Khuseeya	Waxay la mid noqon kartaa qalabka PCR-ka ee fluorescent-ka ee suuqa yaalNidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamyada PCR-ga ee ABI 7500 ee waqtiga-dhabta ah Nidaamyada PCR-ga ee ABI 7500 ee Degdegga ah ee Waqtiga-dhabta ah QuantStudio^®5 Nidaam oo PCR-Waqtiga-dhabta ah LightCycler^®Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX96, BioRad Nidaamka PCR-ga ee Waqtiga-dhabta ah ee CFX Opus 96