Lix nooc oo ah cudur-sidaha neef-mareenka

Sharaxaad Gaaban:

Qalabkan waxa loo isticmaali karaa in si tayo leh lagu ogaado aashitada nucleic ee SARS-CoV-2, fayraska hargabka A, fayraska hargabka B, adenovirus, mycoplasma pneumoniae iyo fayraska syncytial neefsiga ee vitro.


Faahfaahinta Alaabta

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Magaca alaabta

HWTS-OT058A/B/C/Z-Xirmada saxda ah ee fluorescent RT-PCR oo lagu ogaanayo lix nooc oo cudur-sidaha neef-mareenka

Shahaado

CE

Epidemiology

Cudurka Corona Virus 2019, oo loo yaqaan "COVID-19", waxaa loola jeedaa oof wareenka uu keeno caabuqa SARS-CoV-2.SARS-CoV-2 waa coronavirus ka tirsan β-genus.COVID-19 waa cudur faafa oo neef-mareenka oo ba'an, dadkuna guud ahaan waa u nugul yihiin.Waqtigan xaadirka ah, isha infekshanku waa inta badan bukaanada uu haleelay SARS-CoV-2, dadka asymptomatic-ka qaba ayaa sidoo kale noqon kara isha caabuqa.Iyada oo ku saleysan baaritaanka cudurrada faafa ee hadda, muddada soo-kabashada waa 1-14 maalmood, badiyaa 3-7 maalmood.Qandho, qufac qallalan iyo daal ayaa ah calaamadaha ugu waaweyn.Bukaanno dhowr ah ayaa lahaa sanka oo ciriiri ah, diif, cune xanuun, myalgia iyo shuban.

Hargabka, oo loo yaqaan "flu", waa cudur faafa oo neef-mareenka oo ba'an oo uu keeno fayraska hargabka.Aad buu u faafayaa.Inta badan waxa lagu kala qaadaa qufaca iyo hindhisada.Caadiyan waxay soo baxdaa guga iyo jiilaalka.Fayrasyada hargabku waxay u qaybsan yihiin hargab A, IFV A, hargabka B, IFV B, iyo Hargabka C, IFV C saddex nooc, dhammaantood waxay ka tirsan yihiin fayras dhegdheg ah, waxay sababaan cudurrada bini'aadamka inta badan fayraska hargabka A iyo B, waa hal-ganacsi. fayraska RNA qaybsan.Hargabka A waa caabuq neef-mareenka oo degdeg ah, oo ay ku jiraan H1N1, H3N2 iyo noocyo kale oo hoose, kuwaas oo u nugul isbeddelka iyo dillaaca adduunka oo dhan."Shift" waxaa loola jeedaa beddelka fayraska hargabka A, taasoo keentay soo bixitaanka fayraska cusub "subtype".Fayrasyada hargabka B waxa loo qaybiyaa laba abtirsiimo, Yamagata iyo Victoria.Fayraska hargabka B waxa uu leeyahay oo kaliya fayraska antigenic, waxana uu ka baxsadaa ilaalinta iyo baabi'inta habka difaaca bini'aadamka iyada oo loo marayo isbeddelkiisa.Si kastaba ha ahaatee, xawaaraha kobaca ee fayraska hargabka B wuu ka gaabiyaa kan fayraska hargabka A.Fayraska hargabka B wuxuu kaloo keeni karaa caabuqyada neef-mareenka ee bani'aadamka wuxuuna u horseedi karaa cudurro faafa.

Adenovirus (AdV) waxaa iska leh adenovirus naasleyda, kaas oo ah fayraska DNA-da labanlaaban ee aan baqshadda lahayn.Ugu yaraan 90 genotypes ayaa la helay, kuwaas oo loo qaybin karo AG 7 subgenera.Caabuqa AdV wuxuu keeni karaa cudurro kala duwan, oo ay ku jiraan oof wareenka, boronkiitada, cystitis, conjunctivitis indhaha, cudurada caloosha iyo encephalitis.Adenovirus oof wareenka waa mid ka mid ah noocyada aadka u daran ee sambabada sambabada ee bulshadu ka hesho carruurta, oo ka dhigan qiyaastii 4% -10% oof wareenka bulshadu hesho.

Mycoplasma pneumoniae (MP) waa nooc ka mid ah microorganism-ka ugu yar ee prokaryotic, kaas oo u dhexeeya bakteeriyada iyo fayraska, oo leh qaab dhismeed unug laakiin aan lahayn derbi unug.MP ayaa inta badan sababa caabuqa habka neef-mareenka ee bini'aadmiga, gaar ahaan carruurta iyo dhalinyarada.Waxa ay sababi kartaa oof wareenka mycoplasma biniaadmiga, caabuqa mareenka neef-mareenka ee carruurta iyo oof wareenka aan caadiga ahayn.Calaamadaha caafimaadku waa kala duwan yihiin, kuwaas oo intooda badan ay yihiin qufac daran, qandho, qarqaryo, madax-xanuun, cune xanuun.Caabuqa hawada sare ee neef-mareenka iyo sambabada bronki ayaa ah kuwa ugu badan.Bukaanjiifka qaarkood waxay ka qaadi karaan caabuqa neef-mareenka sare ilaa oof wareen daran, cidhiidhi neefsi oo daran iyo dhimasho ayaa dhici karta.

Fayraska isku dhafan ee neefsashada (RSV) waa fayras RNA, oo ka tirsan qoyska paramyxoviridae.Waxaa lagu kala qaadaa dhibco hawo ah iyo xiriir dhow waana cudur keenaha ugu weyn ee caabuqa mareenka neef-mareenka hoose ee dhallaanka.Dhallaanka uu ku dhacay RSV waxaa laga yaabaa inay yeeshaan bronchiolitis daran (oo loo yaqaanno bronchiolitis) iyo oof-wareen, kuwaas oo la xiriira neefta carruurta.Dhallaanku waxay leeyihiin calaamado aad u daran, oo ay ku jiraan qandho sare, rhinitis, pharyngitis iyo laryngitis, ka dibna bronkiolitis iyo oof-wareen.Dhawr carruur ah oo buka ayaa ku adkaan kara otitis media, pleurisy iyo myocarditis, iwm. Infekshanka mareenka sare ee neef-mareenka waa calaamadda ugu weyn ee caabuqa ee dadka waaweyn iyo carruurta ka weyn.

Kanaalka

Magaca kanaalka R6 Reaction Buffer A R6 Reaction Buffer B
FAM SARS-CoV-2 HADV
VIC/HEX Xakamaynta Gudaha Xakamaynta Gudaha
CY5 IFV A MP
ROX IFV B RSV

Qiyaasaha Farsamada

Kaydinta Dareeraha: ≤-18℃ Madow;Lyophilized: ≤30℃ Madaw
Nolosha shelf Dareeraha: 9 bilood;Lyophilized: 12 bilood
Nooca Tuunada Dhiig dhan, Plasma, Serum
Ct ≤38
CV ≤5.0
LoD 300 koobi/ml
Gaar ahaan Natiijooyinka falcelinta-is-dhaafsiga waxay muujiyeen inaysan jirin fal-celin isku-dhafan oo u dhexeeya xirmada iyo coronavirus-ka aadanaha SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, fayraska parainfluenza nooca 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus aadanaha, enterovirus A, B, C, D, fayraska sambabada aadanaha, fayraska epstein-barr, fayraska jadeecada, virus cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella xiiq-dheerta, hargabka haemophilus, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, qiiqa aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci iyo cryptococcus dhawaan dhashay iyo genomic nucleic acid aadanaha.
Qalabka lagu dabaqi karo Waxay la jaan qaadi kartaa qalabka PCR-ka ee caadiga ah ee suuqaSLAN-96P Nidaamyada PCR-waqtiga-dhabta ah
ABI 7500 Nidaamyada PCR-waqtiga-dhabta ah
ABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh
QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah
LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah
LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha
MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga
BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad
CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah

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