SARS-CoV-2/hargabka A/hargabka B

Sharaxaad Gaaban:

Qalabkani wuxuu ku habboon yahay in vitro lagu ogaado tayada tayada leh ee SARS-CoV-2, hargabka A iyo hargabka B nucleic acid ee sanbabada sanka iyo sanbabada suufka oropharyngeal kuwaas oo ka mid ah dadka looga shakiyay caabuqa SARS-CoV-2, hargabka A iyo hargabka. B. Waxa kale oo loo isticmaali karaa oof-wareenka looga shakisan yahay iyo kiisaska koox-kooxeedka looga shakisan yahay iyo si loo ogaado tayada iyo aqoonsiga SARS-CoV-2, hargabka A iyo hargabka B nucleic acid ee sanka sanka iyo sanbalada suufka oropharyngeal ee caabuqa Coronavirus ee novel xaalado kale.

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Faahfaahinta Alaabta

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Magaca alaabta

HWTS-RT148-SARS-CoV-2/hargabka A/hargabka B Nucleic Acid Isku darka Baahinta Qalabka (Fluorescence PCR)

Kanaalka

Magaca Kanaalka	PCR-Mix 1	PCR-Mix 2
FAM Channel	ORF1ab hidde	IVA
Kanaalka VIC/HEX	Xakamaynta gudaha	Xakamaynta gudaha
Kanaalka CY5	N hiddo-wadaha	/
Kanaalka ROX	E hidaha	IVB

Qiyaasaha Farsamada

Kaydinta	-18℃
Nolosha shelf	12 bilood
Nooca Tuunada	Nasopharyngeal swabs iyo suufka oropharyngeal
Bartilmaameedka	SARS-CoV-2 saddex bartilmaameed (Orf1ab, N iyo E hiddo-wadaha) / hargabka A / hargabka B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2: 300 koobi/ml Fayraska hargabka A: 500 koobi/mL Fayraska hargabka B: 500 koobi/mL
Gaar ahaan	a) Natiijooyinka tijaabada iskutallaabtu waxay muujisay in qalabku uu ku habboon yahay coronavirus bini'aadamka SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, fayraska syncytial neefsashada A iyo B, fayraska parainfluenza 1, 2 iyo 3, rhinovirusA, B iyo C, adenovirus 1, 2, 3, 4, 5, 7 and 55, human metapneumovirus, enterovirus A, B, C iyo D, fayraska sambabada sambabada ee cytoplasmic, fayraska EB, fayraska jadeecada Human cytomegalovirus, rotavirus, norovirus, fayraska qaamo-qashiirta, fayraska varicella zoster, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, xiiq-dheerta, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, pmigabtecoccus pneumonia. Candida albicans, Candida glabrata Ma jirin falcelinta u dhaxaysa Pneumocystis yersini iyo Cryptococcus neoformans. b) Awoodda ka-hortagga faragelinta: dooro mucin (60mg/ml), 10% (V/V) dhiiga dadka, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (oo ka kooban preservative) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/ml), triamcinolone acetonide (2mg/ml), budesonide (2mg/ml), mometasone (2mg/ml), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/ml), pramivir (1mg/mL), lopinavir (500mg/mL). Ritonavir (60mg/ml), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/ml), levofloxacin (10μg/ml) iyo tobramycin (0.6mg/mL) .Natiijooyinku waxay muujiyeen in walxaha faragelinta ee kor ku xusan aysan lahayn wax jawaab celin ah oo ku saabsan natiijooyinka ogaanshaha cudurada.
Qalabka lagu dabaqi karo	Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah SLAN^®-96P Nidaamyada PCR-waqtiga-dhabta ah QuantStudio™ 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler^®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah