SARS-CoV-2/hargabka A/hargabka B
Magaca alaabta
HWTS-RT148-SARS-CoV-2/hargabka A/hargabka B Nucleic Acid Isku-dhafan Qalabka Garashada (Fluorescence PCR)
Kanaalka
| Magaca Kanaalka | PCR-Mix 1 | PCR-Mix 2 |
| FAM Channel | ORF1ab hidde | IVA |
| Kanaalka VIC/HEX | Xakamaynta gudaha | Xakamaynta gudaha |
| Kanaalka CY5 | N hiddo-wadaha | / |
| Kanaalka ROX | E hidaha | IVB |
Qiyaasta Farsamada
| Kaydinta | -18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Nasopharyngeal swabs iyo suufka oropharyngeal |
| Bartilmaameedka | SARS-CoV-2 saddex bartilmaameed (Orf1ab, N iyo E hiddo-wadaha) / hargabka A / hargabka B |
| Ct | ≤38 |
| CV | ≤10.0% |
| LoD | SARS-CoV-2: 300 koobi/ml Fayraska hargabka A: 500 koobi/mL Fayraska hargabka B: 500 koobi/mL |
| Gaar ahaan | a) Natiijooyinka tijaabada iskutallaabtu waxay muujisay in qalabku uu ku habboon yahay coronavirus bini'aadamka SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, fayraska syncytial neef-mareenka A iyo B, fayraska parainfluenza 1, 2 iyo 3, rhinovirus, 3, 3, 3, 4, 3, 3, 3, 3, 3, 3, 3, 4, rhinovirus, iyo ade2virus. 5, 7 iyo 55, metapneumovirus aadanaha, enterovirus A, B, C iyo D, fayraska sambabada sambabada ee cytoplasmic, EB fayraska, fayraska jadeecada Human cytomegalovirus, rotavirus, norovirus, fayraska qaamo-qashiir, fayraska varicella zoster, Mycoplasma pneumoniae, Chlamydia, pneumoniae. hargabka, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, qaaxada Mycobacterium, Aspergillus fumigatus, Candida albicans, Candida glabrata Ma jirin falcelin u dhaxaysa Pneumocystis yersinioform iyo Cryptoans. b) Awoodda ka hortagga faragelinta: dooro mucin (60mg/ml), 10% (V/V) dhiiga bini'aadamka, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (oo ka kooban preservative) (20mg/ml), beclomethasone (20mg/mL), dexamethasone (20mg/mL0), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/m. (1mg/mL), lopinavir (500mg/ml), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/ml), ceprotene (40μg/ml) Meropenem (200mg/ml), levofloxacin (10μg/mL) iyo levofloxacin (10μg/mL) Natiijooyinku waxay muujiyeen in walxaha faragelinta ee kor ku xusan aysan lahayn wax jawaab celin ah oo ku saabsan natiijooyinka ogaanshaha cudurada. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah SLAN ®-96P Nidaamyada PCR-waqtiga-dhabta ah QuantStudio™ 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Wadarta Xalka PCR
