Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Magaca alaabta
Qalabka Ogaanshaha Aashitada Nukliyeerka ee HWTS-OT062 Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) (Fluorescence PCR)
Shahaadada
CE
Cudurrada Faafa
Staphylococcus aureus waa mid ka mid ah bakteeriyada muhiimka ah ee keenta caabuqa nosocomial. Staphylococcus aureus (SA) waxay ka tirsan tahay staphylococcus waana wakiil ka ah bakteeriyada Gram-positive, taas oo soo saari karta noocyo kala duwan oo sun ah iyo ensaymo duullaan ah. Bakteeriyadu waxay leedahay astaamo faafin ballaaran, cudur-sidaha xooggan iyo heerka iska caabbinta sare. Hidde-sideyaasha nuclease-ka ee heerkulbeegga ah (nuc) waa hidde-side aad loo ilaaliyo oo staphylococcus aureus ah.
Kanaal
| FAM | Hiddo-wadaha mecA ee u adkaysta methicillin |
| ROX | Xakamaynta Gudaha |
| CY5 | hiddo-wadaha Staphylococcus aureus nuc |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ & laga ilaaliyo iftiinka |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | xaako, muunado caabuq maqaarka iyo unugyada jilicsan, iyo muunado suuf sanka ah |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bakteeriyada u adkaysta methicillin. Marka qalabku ogaado tixraaca qaranka ee LoD, 1000/mL staphylococcus aureus waa la ogaan karaa. |
| Gaar ahaanshaha | Tijaabada falcelinta isdhaafka ah waxay muujinaysaa in qalabkani uusan lahayn falcelin isdhaafsi ah oo lala sameeyo jeermisyada kale ee neefsashada sida methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P LightCycler®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaarka 1aad.
Qalabka DNA/RNA ee Macro & Micro-Test Genomic (HWTS-3019) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. waxaa loo isticmaali karaa Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ku dar 200µL oo cusbo caadi ah qulqulka la farsameeyay, tallaabooyinka xigana waa in la soo saaraa iyadoo la raacayo tilmaamaha, mugga dheecaanka lagu taliyayna waa 80µL.
Ikhtiyaarka 2aad.
Macro & Micro-Test Release Tusaalaha Soo-saarista (HWTS-3005-8) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 1mL oo cusbo caadi ah qulqulka ka dib markaad ku dhaqdo cusbo caadi ah, ka dibna si fiican isku qas. Ku shub 13,000r/daqiiqo muddo 5 daqiiqo ah, ka saar dheecaanka (kaydso 10-20µL oo dheecaanka ah), oo raac tilmaamaha soo-saarista xiga.
Waxyaabaha lagu taliyay ee soo saarista: Soo saarista Aashitada Nukliyeerka ama Reagent-ka Nadiifinta (YDP302) oo ay samaysay Tiangen Biotech (Beijing) Co., Ltd. Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tallaabada 2aad ee buug-gacmeedka tilmaamaha. Waxaa lagu talinayaa in la isticmaalo biyo aan lahayn RNase iyo DNase si loogu shubo mugga 100µL.
