Qalabka Ogaanshaha Aashitada Nucleic ee Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus
Magaca alaabta
Qalabka Ogaanshaha Aashitada Nucleic ee Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus (Fluorescence PCR)
Isticmaalka Loogu Talogalay
Qalabkan waxaa loo isticmaalaa ogaanshaha tayada ee staphylococcus aureus iyo asiidhyada methicillin-ka u adkaysta staphylococcus aureus nucleic ee muunadaha candhuufta aadanaha, muunadaha sanka iyo muunadaha caabuqa maqaarka iyo unugyada jilicsan ee in vitro. Baaritaanka muunada candhuufta iyo suufka sanka waxaa loo isticmaalaa xarumaha caafimaadka si looga hortago loona xakameeyo caabuqa nosocomial ee MRSA ee bukaanada isbitaalka la dhigay, oo ay ku jiraan bukaanada daryeelka degdegga ah, bukaanada qalliinka iyo bukaanada daryeelka muddada dheer; Waxaa sidoo kale loo isticmaali karaa ogaanshaha kaalmaynta ee bukaanada looga shakisan yahay Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus ee caabuqa neefsashada; Ama iyadoo lala kaashanayo baaritaanno kale oo shaybaar sida dhaqanka microbial si looga caawiyo ogaanshaha caabuqyada maqaarka iyo unugyada jilicsan ee MRSA/SA. Natiijooyinka baaritaanka si toos ah looguma isticmaalo hagitaanka isticmaalka antibiyootikada.
Cudurrada Faafa
Staphylococcus aureus waa mid ka mid ah bakteeriyada muhiimka ah ee keenta caabuqa nosocomial. Staphylococcus aureus (SA) waxay ka tirsan tahay staphylococcus waana wakiil ka ah bakteeriyada Gram-positive, taas oo soo saari karta noocyo kala duwan oo sun ah iyo ensaymo duullaan ah. Bakteeriyadu waxay leedahay astaamo faafin ballaaran, cudur-sidaha xooggan iyo heerka iska caabbinta sare. Hidde-sideyaasha nuclease-ka ee heerkulbeegga ah (nuc) waa hidde-side aad loo ilaaliyo oo staphylococcus aureus ah.
Sannadihii ugu dambeeyay, iyadoo la adeegsanayo hoormoonada iyo diyaarinta difaaca jirka iyo ku xadgudubka antibiyootiga ballaaran ee ballaaran, caabuqyada nosocomial ee ay keento Staphylococcus aureus (MRSA) ee u adkaysta Methicillin ee Staphylococcus ayaa sii kordhayay. Celceliska heerka ogaanshaha MRSA ee qaranka wuxuu ahaa 30.2% sannadkii 2019 ee Shiinaha. MRSA waxaa loo qaybiyaa MRSA oo la xiriirta daryeelka caafimaadka (HA-MRSA), MRSA oo la xiriirta bulshada (CA-MRSA), iyo MRSA oo la xiriirta xoolaha (LA-MRSA). CA-MRSA, HA-MRSA, LA-MRSA waxay leeyihiin farqi weyn oo ku saabsan microbiology, iska caabbinta bakteeriyada (tusaale ahaan, HA-MRSA waxay muujinaysaa iska caabbinta daawooyinka badan marka loo eego CA-MRSA) iyo astaamaha kiliinikada (tusaale ahaan goobta caabuqa). Sida laga soo xigtay astaamahan, CA-MRSA iyo HA-MRSA waa la kala saari karaa. Si kastaba ha ahaatee, farqiga u dhexeeya CA-MRSA iyo HA-MRSA ayaa sii yaraanaya sababtoo ah dhaqdhaqaaqa joogtada ah ee dadka u dhexeeya isbitaallada iyo bulshooyinka. MRSA waa mid u adkaysta daawooyinka badan, ma aha oo kaliya inay u adkaysato antibiyootikada β-lactam, laakiin sidoo kale waxay u adkaystaa aminoglycosides, macrolides, tetracyclines iyo quinolones heerar kala duwan. Waxaa jira kala duwanaansho goboleed oo ballaaran oo ku saabsan heerarka iska caabbinta daawada iyo isbeddellada kala duwan. MRSA waxay ku soo duuli kartaa jirka iyada oo loo marayo nabarrada maqaarka, timaha timaha, dhiigga iyo sababo kale oo keena caabuq suppurative ah, bukaanada qaba cudurrada maqaarka iyo gubashadana aad bay ugu nugul yihiin caabuqa MRSA. Oof-wareenku waa mid ka mid ah caabuqyada caafimaad ee ugu badan ee MRSA, daaweynta aan habboonaynna waa sabab muhiim ah oo keenta dhimasho sare.
Halbeegyada Farsamada
| Kaydinta | ≤ -18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Xaako, suufka sanka, muunadda caabuqa maqaarka iyo unugyada jilicsan, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Gaar ahaanshaha | Tijaabada falcelinta isdhaafka ah waxay muujinaysaa in qalabkani uusan lahayn falcelin isdhaafsi ah oo lala sameeyo jeermisyada kale ee neefsashada sida methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Qalabka Khuseeya | Nooca I: Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee Bayoolajiga 7500, Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee QuantStudio®5, Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Nidaamka Ogaanshaha PCR-ka Waqtiga-dhabta ah (FQD-96A, Tiknoolajiyadda Hangzhou Bioer), MA-6000 Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee Tiro-koobka (Suzhou Molarray Co., Ltd.), Nidaamka PCR-ka ee Waqtiga-dhabta ah ee BioRad CFX96, Nidaamka PCR-ka ee Waqtiga-dhabta ah ee BioRad CFX Opus 96. Nooca II: HWTS-EudemonTM AIO800 |
Socodka Shaqada
Reagents iyo qalabku waxay u baahan yihiin laakiin lama bixin
Nooca I
(1) Qalabka Guud ee Baaritaanka Macro & Micro-Test DNA/RNA (HWTS-3019) oo ka socda Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Waxaa loo isticmaali karaa Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Saambal milmiye: 4% NaOH ama Sputasol oo ka socda Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Cusbo caadi ah.
Nooca II:
Qalabka Guud ee Baaritaanka Yaryar iyo Yaryar ee DNA/RNA (HWTS-3019-8) oo ka socda Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Qalabka la isticmaalo waa loo baahan yahay laakiin lama bixin:Talooyin aan RNase/DNase lahayn, galoofisyo la tuuri karo, tuubooyinka EP ee aan RNase/DNase lahayn, xariijimo 8-tuubo ah oo loogu talagalay PCR.
