Cudurka Ebola ee Zaire
Magaca alaabta
HWTS-FE008 Zaire Ebola Virus Qalabka lagu ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Fayraska Ebola waxaa iska leh Filoviridae, kaas oo ah fayraska RNA-negative-strand oo aan kala qaybsanayn. Fayrasyadu waa fiilooyin dhaadheer oo celcelis ahaan dhererkoodu yahay 1000nm dhexroorkiisuna yahay 100nm. genome-ka fayraska Ebola waa RNA-xarun taban oo aan kala qaybsanayn oo cabbirkeedu dhan yahay 18.9kb, kaas oo dhigaya 7 borotiinno qaab-dhismeed ah iyo 1 borotiin aan dhisme ahayn. Fayraska Ebola waxa loo qaybin karaa noocyo ay ka mid yihiin Zaire, Sudan, Bundibugyo, Tai Forest iyo Reston. Waxaa ka mid ah, nooca Zaire iyo nooca Sudan ayaa la sheegay inay sababeen dad badan inay u dhintaan caabuqa. EHF (Ebola Hemorrhagic Fever) waa cudur faafa oo dhiigbax ah oo ba'an oo uu keeno fayraska Ebola. Bani'aadamka waxaa inta badan ku dhaca taabashada dareeraha jirka, dheecaannada iyo saxarada bukaannada ama xayawaanka qaba cudurka, calaamadaha bukaan-socodka ayaa inta badan ah qandho soo baxda, dhiigbax iyo dhaawac badan oo xubno ah. EHF waxay leedahay heerka dhimashada sare ee 50%-90%.
Kanaalka
| FAM | MP nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Serum cusub, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/μL |
| Gaar ahaan | Isticmaal qalabka si aad u tijaabiso tixraacyada xun ee shirkadda, natiijadu waxay buuxisaa shuruudaha. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, iyo BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Soo saarista lagu taliyay: QIAamp Viral RNA Mini Kit (52904), Soosaarka Asiidhka Nukliyeerka ama Reagent Nadiifinta (YDP315-R) ee Tiangen Biotech (Beijing) Co., Ltd. Waa in si adag loo soo saaro si waafaqsan tilmaamaha, iyo mugga soo saarista lagu taliyey ee muunada waa 140μL iyo mugga elution ee lagu taliyey waa 60μL.
Doorashada 2.
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) .Waa in la soosaaraa si waafaqsan tilmaamaha. Mugga muunadda soo saariddu waa 200μL, mugga elution ee lagu taliyeyna waa 80μL.
Doorashada 3.
Reagents soo saarista lagu taliyay: Nucleic Acid Reagent Soo saarista (1000020261) iyo Nidaamka Diyaarinta Muunada Automated Sare (MGISP-960) ee BGI waa in lagu soo saaraa si waafaqsan tilmaamaha. Mugga soo-saarku waa 160μL, mugga sare-u-qaadista ee lagu taliyeyna waa 60μL.
