18 Noocyada Khatarta Sare leh ee Fayraska papilloma ee Bani'aadamka Nucleic Acid
Magaca alaabta
HWTS-CC018B-18 Noocyada Khatarta Sare leh ee Bani'aadamka Papilloma Virus-ka Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Shahaado
CE
Epidemiology
Kansarka ilmo-galeenka waa mid ka mid ah burooyinka halista ah ee ugu badan ee ku dhaca habka taranka haweenka.Daraasaduhu waxay muujiyeen in caabuqa joogtada ah iyo caabuqyada badan ee papillomavirus ee bini'aadamka ay yihiin mid ka mid ah sababaha muhiimka ah ee kansarka ilmo-galeenka.
Mareenka taranka infekshanka HPV wuxuu ku badan yahay haweenka nolosha galmoodka leh.Marka loo eego tirakoobyada, 70% ilaa 80% haweenka waxaa laga yaabaa inay qaadaan caabuqa HPV hal mar ugu yaraan inta ay nool yihiin, laakiin inta badan infekshannada waa kuwo iskood u xaddidan, in ka badan 90% haweenka cudurka qaba waxay yeelan doonaan jawaab celin difaac oo waxtar leh oo nadiifin karta caabuqa. inta u dhaxaysa 6 iyo 24 bilood iyada oo aan wax faragelin caafimaad oo waqti dheer ah la helin.Caabuqa HPV ee khatarta sare leh ee joogtada ah ayaa ah sababta ugu weyn ee neoplasia intraepithelial neoplasia iyo kansarka ilmo-galeenka.
Natiijooyinka cilmi-baarista adduunka oo dhan waxay muujiyeen in jiritaanka khatarta sare ee DNA-da HPV lagu ogaaday 99.7% bukaannada kansarka ilmo-galeenka.Sidaa darteed, ogaanshaha hore iyo ka-hortagga HPV-ga ilmo-galeenka ayaa fure u ah joojinta kansarka.Samaynta hab sahlan, gaar ah oo degdeg ah oo ogaanshaha cudur-sidaha ayaa muhiimad weyn u leh ogaanshaha bukaan-socodka ee kansarka ilmo-galeenka.
Kanaalka
| FAM | HPV 18 |
| VIC (HEX) | HPV 16 |
| ROX | HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82 |
| CY5 | Xakamaynta gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ gudcurka |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Xuubka afka ilmagaleenka, suufka siilka, kaadida |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 300 koobi/ml |
| Gaar ahaan | (1) Walxaha soo dhex gala Isticmaal xirmooyinka si aad u tijaabiso walxaha soo socda ee soo horjeeda, natiijadu waa dhammaan diidmo: hemoglobin, unugyada dhiigga cad, xabka ilmo-galeenka, metronidazole, jieryin lotion, Fuyanjie lotion, saliidda aadanaha.(2) Falcelinta-celinta Isticmaal qalabka si aad u tijaabiso cudurada kale ee la xidhiidha mareenka taranka iyo DNA-da genomic ee laga yaabo in ay falcelis ku yeeshaan xirmooyinka, natiijadu waa wada taban: HPV6 muunadaha togan, HPV11 muunadaha togan, HPV40 togan, HPV42 togan, HPV43 , HPV44 togan, HPV54, HPV54, HPV67, HPV69, HPV70, HPV71, HPV72, HPV81, HPV83, nooca fayraska herpes simplex, nooca Ⅱ, treponema pallidum, ureacoplasmum hominis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, chlamydia trachomatis iyo DNA genomic |
| Qalabka lagu dabaqi karo | SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Wadarta Xalka PCR
Doorashada 1.
1. Muunad
2. Nucleic acid soo saarista
3. Ku dar muunado mashiinka
Doorashada 2.
1. Muunad
2. Wax-soosaar la'aan
3. Ku dar muunado mashiinka
