18 Noocyada Khatarta Sare leh ee Fayraska papilloma ee Bani'aadamka Nucleic Acid
Magaca alaabta
HWTS-CC018B-18 Noocyada Khatarta Sare leh ee Bani'aadamka Papilloma Virus-ka Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Shahaado
CE
Epidemiology
Kansarka ilmo-galeenka waa mid ka mid ah burooyinka halista ah ee ugu badan ee ku dhaca habka taranka haweenka. Daraasaduhu waxay muujiyeen in caabuqa joogtada ah iyo caabuqyada badan ee papillomavirus ee bini'aadamka ay yihiin mid ka mid ah sababaha muhiimka ah ee kansarka ilmo-galeenka.
Mareenka taranka infekshanka HPV wuxuu ku badan yahay haweenka nolosha galmoodka leh. Marka loo eego tirakoobyada, 70% ilaa 80% haweenka waxaa laga yaabaa inay qaadaan caabuqa HPV hal mar ugu yaraan noloshooda, laakiin inta badan caabuqyada ayaa ah kuwo iskood u xaddidan, in ka badan 90% haweenka cudurka qaba waxay yeelan doonaan jawaab celin wax ku ool ah oo difaac ah oo nadiifin kara caabuqa inta u dhaxaysa 6 iyo 24 bilood iyada oo aan wax faragelin caafimaad oo dheer ah la helin. Caabuqa HPV ee khatarta sare leh ee joogtada ah ayaa ah sababta ugu weyn ee neoplasia intraepithelial neoplasia iyo kansarka ilmo-galeenka.
Natiijooyinka cilmi-baarista adduunka oo dhan waxay muujiyeen in jiritaanka khatarta sare ee HPV DNA lagu ogaaday 99.7% bukaannada kansarka ilmo-galeenka. Sidaa darteed, ogaanshaha hore iyo ka-hortagga HPV-ga ilmo-galeenka ayaa fure u ah joojinta kansarka. Samaynta habka ogaanshaha cudur-sidaha ee fudud, gaar ah oo degdeg ah ayaa muhiimad weyn u leh ogaanshaha bukaan-socodka ee kansarka ilmo-galeenka.
Kanaalka
| FAM | HPV 18 |
| VIC (HEX) | HPV 16 |
| ROX | HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82 |
| CY5 | Xakamaynta gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ gudcurka |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Xuubka afka ilmagaleenka, suufka siilka, kaadida |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 300 koobi/ml |
| Gaar ahaan | (1) Walxaha soo dhex gala Isticmaal xirmooyinka si aad u tijaabiso walxaha soo socda ee soo horjeeda, natiijadu dhammaantood waa taban: hemoglobin, unugyada dhiigga cad, xabka ilmo-galeenka, metronidazole, lotion Jieryin, lotion Fuyanjie, saliidaha aadanaha.(2) Falcelinta-celinta Isticmaal xirmooyinka si aad u tijaabiso cudurada kale ee la xidhiidha mareenka taranka iyo DNA-da genomic ee laga yaabo in ay falcelis ku yeeshaan xirmooyinka, natiijadu waa wada taban: HPV6, HPV11, HPV40, HPV40, HPV40, HPV43, HPV43 shaybaarada, HPV71 togan, HPV72 muunadaha togan, HPV81 muunadaha togan, HPV83 muunadaha togan, fayraska herpes simplex nooca Ⅱ, treponema pallidum, ureaplasma urealyticum, mycoplasma hominis, candida albicans, neisseria gonorrhoeae, neisseria gonorrhoeae, bini'aadamka trachomamytis vania geronorrhea. |
| Qalabka lagu dabaqi karo | SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Wadarta Xalka PCR
Doorashada 1.
1. Muunad
2. Nucleic acid soo saarista
3. Ku dar muunado mashiinka
Doorashada 2.
1. Muunad
2. Wax-soosaar la'aan
3. Ku dar muunado mashiinka
