28 Nooc oo ah Qalabka Baaritaanka Aashitada Nukliyeerka ee HPV (Fluorescence PCR)
Magaca alaabta
28 Nooc oo ah Qalabka Baaritaanka Aashitada Nukliyeerka ee HPV (Fluorescence PCR)
Cudurrada Faafa
Fayraska Human Papillomavirus (HPV) wuxuu ka tirsan yahay qoyska Papillomaviridae ee molecule yar, aan la daboolin, oo wareegsan oo fayras DNA ah oo laba jibbaaran, oo dhererkiisu yahay hidde-side qiyaastii 8000 oo lammaane saldhig ah (bp). HPV wuxuu ku dhacaa aadanaha iyada oo loo marayo taabasho toos ah ama aan toos ahayn oo lala yeesho walxaha wasakhaysan ama gudbinta galmada. Fayrasku ma aha oo kaliya mid gaar u ah martida, laakiin sidoo kale waa mid gaar u ah unugyada, wuxuuna ku dhici karaa oo keliya maqaarka aadanaha iyo unugyada epithelial-ka xuubka, isagoo sababa noocyo kala duwan oo papillomas ama burooyinka maqaarka aadanaha iyo dhaawac faafa oo ku dhaca epithelium-ka habka taranka.
Kansarka ilmo-galeenka waa mid ka mid ah burooyinka ugu badan ee ku dhaca habka taranka dumarka. Daraasaduhu waxay muujiyeen in caabuqyada joogtada ah ee HPV iyo caabuqyada badan ay yihiin mid ka mid ah sababaha ugu waaweyn ee kansarka ilmo-galeenka. Waqtigan xaadirka ah, daawaynta waxtarka leh ee la aqoonsan yahay weli ma jiraan kansarka ilmo-galeenka ee uu keeno HPV, sidaa darteed helitaanka hore iyo ka hortagga caabuqa ilmo-galeenka ee uu sababo HPV waa furaha ka hortagga kansarka ilmo-galeenka. Waa muhiimad weyn in la sameeyo baaritaan fudud, gaar ah oo degdeg ah oo lagu ogaanayo cudurka kansarka ilmo-galeenka si loo ogaado cudurka iyo daaweynta kansarka ilmo-galeenka.
Kanaal
| S/N | Kanaal | Nooca |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC (HEX) | Xakamaynta Gudaha | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC (HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
Halbeegyada Farsamada
| Kaydinta | ≤ -18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Kaadida, muunadda suufka afka ilmo-galeenka dumarka, muunadda suufka siilka dumarka |
| CV | <5.0% |
| LoD | 300 Nuqul/mL |
| Gaar ahaanshaha | Ma jiro wax falcelin isdhaafsi ah oo lala yeesho jeermisyada kale ee la xiriira taranka iyo DNA-da hidde-sidaha aadanaha. |
| Qalabka Khuseeya | Nidaamka PCR-ka ee Waqtiga-dhabta ah ee La Adeegsaday (Applied Biosystems 7500 Real-Time PCR Systems), Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee La Adeegsaday (Applied Biosystems 7500 Fast-Real-Time PCR Systems), Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee QuantStudio®5 Real-Time, Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.), Nidaamyada PCR-ka ee Waqtiga-dhabta ah ee LightCycler®480 Real-Time, Nidaamyada Ogaanshaha PCR-ka ee LineGene 9600 Plus Real-Time (FQD-96A, Tiknoolajiyadda Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. |
Socodka Shaqada: Faahfaahin dheeraad ah ka eeg Qalabka IFU
Xalka 1 (Soo saarista aashitada nukliyeer-ka ee otomaatiga ah)
Xalka 2aad: (Macro & Micro-Test Reagent oo ah wakiilka sii deynta muunadda)
Reagent-yadu waxay u baahan yihiin laakiin lama bixin
Wakiilka Soo-saarista Muunadda ee Macro & Micro-Test (HWTS-3005-8), Qalabka DNA/RNA ee Macro & Micro-Test ee Viral (HWTS-3017) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Qalabka la isticmaalo waa loo baahan yahay laakiin lama bixin
1.5mL oo tuubooyin aan lahayn DNase/RNase, talooyin aan lahayn DNase/RNase, 8-tuubo oo loogu talagalay PCR, centrifuge-ka miiska saaran, isku-darka ruxaha miiska saaran.
