28 Nooc oo Fayraska Papilloma-ga Aadanaha ee Khatarta Sare leh (Qoraalka 16/18) Nucleic Acid

Sharaxaad Gaaban:

Qalabkani wuxuu ku habboon yahay ogaanshaha tayada ee in vitro ee 28 nooc oo fayrasyada papilloma-da aadanaha (HPV) (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) asiidhka nucleic ee kaadida labka/dheddigga iyo unugyada la jaray ee afka ilmo-galeenka dheddigga. HPV 16/18 waa la qori karaa, noocyada soo harayna si buuxda looma qori karo, taasoo bixinaysa hab kaabayaal ah oo lagu ogaanayo laguna daaweynayo caabuqa HPV.

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Faahfaahinta Badeecada

Calaamadaha Alaabta

Magaca alaabta

HWTS-CC006A-28 Noocyada Fayraska Papilloma-ga Aadanaha ee Khatarta Sare leh (Qoraalka 16/18) Qalabka Ogaanshaha Aashitada Nukliyeerka (Fluorescence PCR)

Cudurrada Faafa

Kansarka ilmo-galeenka waa mid ka mid ah burooyinka ugu badan ee ku dhaca habka taranka dumarka. Daraasaduhu waxay muujiyeen in caabuqyada joogtada ah ee HPV iyo caabuqyada badan ay yihiin mid ka mid ah sababaha ugu waaweyn ee kansarka ilmo-galeenka. Waqtigan xaadirka ah, daawaynta waxtarka leh ee la aqoonsan yahay weli ma jiraan kansarka ilmo-galeenka ee uu keeno HPV, sidaa darteed helitaanka hore iyo ka hortagga caabuqa ilmo-galeenka ee uu sababo HPV waa furaha ka hortagga kansarka ilmo-galeenka. Waa muhiimad weyn in la sameeyo baaritaan fudud, gaar ah oo degdeg ah oo lagu ogaanayo cudurka kansarka ilmo-galeenka si loo ogaado cudurka iyo daaweynta kansarka ilmo-galeenka.

Kanaal

Isku-darka Falcelinta	Kanaal	Nooca
PCR-Mix1	FAM	18
	VIC (HEX)	16
	ROX	31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68
	CY5	Xakamaynta Gudaha
PCR-Mix2	FAM	6, 11, 54, 83
	VIC (HEX)	26, 44, 61, 81
	ROX	40, 42, 43, 53, 73, 82
	CY5	Xakamaynta Gudaha

Halbeegyada Farsamada

Kaydinta	Dareere: ≤-18℃
Muddada khaanadaha	12 bilood
Nooca Muunada	Suunka Ilkaha, Suunka Siilka, Kaadida
Ct	≤28
CV	<5.0%
LoD	300 Nuqullo/mL
Qalabka Khuseeya	Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio^®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P LightCycler^®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96

Socodka Shaqada

Waxyaabaha lagu taliyay ee soo saarista: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 200μL oo cusbo caadi ah si aad dib ugu soo celiso pellet-ka tallaabada 2.1, ka dibna soo saarista waa in la sameeyaa iyadoo la raacayo tilmaamaha isticmaalka qalabkan soo saarista. Mugga soo saarista ee lagu taliyay waa 80μL.
Waxyaabaha lagu taliyay ee soo saarista: QIAamp DNA Mini Kit (51304) ama Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Ku dar 200μL oo cusbo caadi ah si aad dib ugu soo celiso pellet-ka tallaabada 2.1, ka dibna soo saarista waa in la sameeyaa iyadoo la raacayo tilmaamaha isticmaalka qalabkan soo saarista. Mugga muunadda ee la soo saaray ee muunadaha dhammaantood waa 200μL, mugga soo saarista ee lagu taliyayna waa 100μL.

Kii hore: Enterococcus-ka u adkaysta Vancomycin iyo hiddo-side u adkaysta daawada

Xiga: Fayraska Papillomavirus ee Aadanaha (28 Nooc)

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