28 Nooc oo Khatarta Sarreeysa ee Fayraska Papilloma Human (16/18 Qorista) Acid Nukliyeer ah
Magaca alaabta
HWTS-CC006A-28 Noocyada Khatarta Sare leh ee Fayraska papilloma ee Aadanaha (16/18 Qorista) Qalabka lagu ogaado Acid Nukliyeer ah (Fluorescence PCR)
Epidemiology
Kansarka ilmo-galeenka waa mid ka mid ah burooyinka halista ah ee ugu badan ee marinka taranka dumarka.Daraasaduhu waxay muujiyeen in caabuqyada HPV ee joogtada ah iyo caabuqyada badan ay yihiin mid ka mid ah sababaha ugu waaweyn ee kansarka ilmo-galeenka.Waqtigan xaadirka ah, daawaynta waxtarka leh ee la aqoonsan yahay ayaa weli ka maqan kansarka ilmo-galeenka ee uu keeno HPV, sidaas darteed hore u ogaanta iyo ka-hortagga caabuqa ilmo-galeenka ee uu keeno HPV waa furaha ka hortagga kansarka ilmo-galeenka.Aad bay muhiim u tahay in la sameeyo baaritaan fudud, gaar ah oo degdeg ah oo ogaanshaha etiology ee ogaanshaha kiliinikada iyo daaweynta kansarka ilmo-galeenka.
Kanaalka
| Isku darka falcelinta | Kanaalka | Nooca |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Xakamaynta Gudaha | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | Dareeraha: ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Xuubka afka ilmagaleenka, suufka siilka, kaadida |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 koobi/ml |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaaso loo isticmaali karo Macro & Micro-Test Soo saarista Ashitada Nucleic Acid (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 200μL ee cusbada caadiga ah si aad dib ugu soo celiso pellet ee tallaabada 2.1, ka dibna soo saarista waa in lagu sameeyaa si waafaqsan Tilmaamaha isticmaalka reagent soo saarida.Mugga sare ee lagu taliyey waa 80μL.
Soo saarista lagu taliyay: QIAamp DNA Mini Kit (51304) ama Makro & Micro-Tijaabo Viral DNA/RNA Column (HWTS-3020-50).Ku dar 200μL oo cusbo ah oo caadi ah si aad dib ugu celiso pellet-ka tallaabada 2.1, ka dibna soo saarista waa in loo fuliyaa si waafaqsan tilmaamaha isticmaalka reagent soo saarista.Mugga muunada la soo saaray ee shaybaarada ayaa dhamaantood ah 200μL, mugga sare ee lagu taliyeyna waa 100μL.
