Enterovirus 71 (EV71)
Magaca alaabta
Qalabka Baadhista Aashitada Nukliyeerka ee HWTS-EV003- Enterovirus 71 (EV71) (Fluorescence PCR)
Cudurrada Faafa
Cudurka afka-gacanta waa cudur faafa oo ay sababaan enteroviruses (EV). Waqtigan xaadirka ah, waxaa la helay 108 nooc oo ah noocyada serotypes-ka ee enteroviruses, kuwaas oo loo qaybiyay afar kooxood: A, B, C iyo D. Kuwaas waxaa ka mid ah enterovirus EV71 iyo CoxA16 waa cudurada ugu waaweyn ee keena. Cudurku wuxuu inta badan ku dhacaa carruurta ka yar 5 sano, wuxuuna ku keeni karaa herpes gacmaha, cagaha, afka iyo qaybaha kale. Tiro yar oo carruur ah ayaa yeelan doona dhibaatooyin sida myocarditis, bararka sambabada, iyo meningoencephalitis aseptic.
Kanaal
| FAM | EV71 |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | Suufka afka,Dareeraha herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Nuqullo/mL |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P LightCycler®Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaarka 1aad.
Waxyaabaha lagu taliyay ee soo saarista: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), soo saaristana waa in loo sameeyaa sida ku cad tilmaamaha loo isticmaalo. Muunadaha la soo saaray waa suufka afka ama muunadaha dareeraha herpes ee bukaanada laga soo ururiyey goobta. Ku dar suufka la soo ururiyay Macro & Micro-Test Sample Reagent (HWTS-3005-8) si toos ah, u rog oo si fiican isku qas, dhig heerkulka qolka 5 daqiiqo, ka saar ka dibna u rog si aad si fiican isugu qasto, si aad u hesho RNA-ga muunad kasta.
Ikhtiyaarka 2aad.
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Guud ee DNA/RNA ee Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., soo saaristana waa in loo sameeyaa sida ku cad tilmaamaha loo isticmaalo. Mugga muunadda la soo saaray waa 200µL, mugga soo saarista ee lagu taliyayna waa 80µL.
Ikhtiyaar 3.
Waxyaabaha lagu taliyay ee soo saarista: QIAamp Viral RNA Mini Kit (52904) oo ay samaysay QIAGEN ama TIAnamp Virus DNA/RNA Kit (YDP315-R), soo saaristana waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha loo isticmaalo. Mugga muunadda la soo saaray waa 140μL, mugga soo saarista ee lagu taliyayna waa 60µL.
