Enterovirus 71 (EV71)
Magaca alaabta
HWTS-EV003-Enterovirus 71 (EV71) Qalabka Ogaanshaha Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Cudurka afka-gacmeedku waa cudur la is qaadsiiyo oo ay keento enteroviruses (EV).Hadda, 108 nooc oo serotypes of enteroviruses ah ayaa la helay, kuwaas oo loo qaybiyay afar kooxood: A, B, C iyo D. Waxaa ka mid ah, enterovirus EV71 iyo CoxA16 waa cudur-sidaha ugu muhiimsan.Cudurku wuxuu inta badan ku dhacaa carruurta da'doodu ka yar tahay 5 sano, wuxuuna ku keeni karaa herpes gacmaha, cagaha, afka iyo qaybaha kale.Tiro yar oo carruur ah ayaa ku dhici doona dhibaatooyin sida myocarditis, bararka sambabada, iyo meningoencephalitis aseptic.
Kanaalka
| FAM | EV71 |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Oropharyngeal suuf,Dheecaan Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 koobi/ml |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Soo saarista lagu taliyay: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8), iyo soo saarista waa in loo fuliyaa si waafaqsan tilmaamaha isticmaalka.Shaybaarada la soo saaray waa suufka oropharyngeal ama muunadaha dareeraha herpes ee bukaanada lagu ururiyay goobta.Ku dar suufyada la ururiyey gudaha Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) si toos ah, vortex oo si fiican isku qas, dhig heerkulka qolka 5 daqiiqo, ka saar ka dibna u rogo si aad si fiican u walaaqdo, si aad u hesho RNA muunad kasta.
Doorashada 2.
Soo saarista lagu taliyay: Macro & Micro-Tijaabada Guud ee DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaaso loo isticmaali karo Macro & Micro-Test Soo saarista Ashitada Nukliyeerka Tooska ah (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., iyo soo saarista waa in loo fuliyaa si waafaqsan tilmaamaha isticmaalka.Mugga muunadda la soo saaray waa 200µL, mugga sareynta lagu taliyeyna waa 80µL.
Ikhtiyaarka3.
Soo saarista lagu taliyay: QIAamp Viral RNA Mini Kit (52904) ee QIAGEN ama TIANAmp Virus DNA/RNA Kit (YDP315-R), iyo soo saarista waa in loo fuliyaa si adag oo waafaqsan tilmaamaha isticmaalka.Mugga muunadda la soo saaray waa 140μL, mugga sareynta lagu taliyeyna waa 60µL.
