Bani'aadamka EGFR Gene 29 Isbeddellada
Magaca alaabta
HWTS-TM001A-Bani'aadamka EGFR Gene 29 Qalabka Daahfurka Isbeddellada (Fluorescence PCR)
Epidemiology
Kansarka sanbabada ayaa noqday sababta ugu badan ee kansarka u dhintaan adduunka oo dhan, oo khatar weyn u geysta caafimaadka aadanaha.Kansarka sambabada ee unugyada aan yareyn ayaa ah qiyaastii 80% bukaannada kansarka sanbabada.EGFR hadda waa bartilmaameedka ugu muhiimsan ee molecular ee daaweynta kansarka sambabada unugyada aan yareyn.Fosforyaalka EGFR waxay kor u qaadi kartaa koritaanka unugyada burooyinka, kala soocida, duulaanka, metastasis, anti-apoptosis, iyo kor u qaadida angiogenesis buro.EGFR tyrosine kinase inhibitors (TKI) waxay xannibi kartaa dariiqa calaamadaynta EGFR iyadoo la joojinayo EGFR autophosphorylation, taas oo joojinaysa faafinta iyo kala soocida unugyada burooyinka, kor u qaadista apoptosis unugyada burooyinka, yaraynta buro angiogenesis, iwm.Tiro badan oo daraasado ah ayaa muujiyay in waxtarka daaweynta ee EGFR-TKI ay si dhow ula xiriirto xaaladda isbeddelka hidda-wadaha EGFR, waxayna si gaar ah u joojin kartaa koritaanka unugyada burooyinka ee leh isbeddellada hidda-wadaha EGFR.Hidde-sidaha EGFR wuxuu ku yaalaa gacanta gaaban ee koromosoomyada 7 (7p12), oo dhererkiisu dhan yahay 200Kb wuxuuna ka kooban yahay 28 exons.Gobolka la bedelay wuxuu inta badan ku yaalaa exon 18 ilaa 21, codons 746 ilaa 753 mutation of exon 19 ayaa u dhiganta 45%Tilmaamaha NCCN ee ogaanshaha iyo daawaynta Kansarka sambabada unugyada aan-yarayn ayaa si cad u qeexaya in baaritaanka hidda-wadaha EGFR looga baahan yahay kahor maamulka EGFR-TKI.Qalabkan tijaabada ah waxaa loo isticmaalaa in lagu hago maamulka dawooyinka tayrosine kinase inhibitor tyrosine kinase inhibitor (EGFR-TKI) ee koritaanka epidermal, iyo in la siiyo aasaaska daawada gaarka ah ee bukaanada qaba kansarka sanbabada unug yar.Xirmadan waxa kaliya oo loo isticmaalaa ogaanshaha isbeddellada caadiga ah ee hidda-wadaha EGFR ee bukaannada qaba kansarka sanbabada unug yar.Natiijooyinka baaritaanka waxaa loogu talagalay tixraaca bukaan-socodka oo keliya mana aha in loo isticmaalo saldhigga keliya ee daaweynta gaarka ah ee bukaanka.Dhakhaatiirtu waa inay tixgeliyaan xaaladda bukaanka, calaamadaha daroogada, iyo daawaynta Falcelinta iyo tilmaamayaasha kale ee shaybaadhka iyo arrimo kale ayaa loo isticmaalaa si loo qiimeeyo natiijooyinka baaritaanka.
Kanaalka
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Qiyaasaha Farsamada
| Kaydinta | Dareeraha: ≤-18℃ Madow;Lyophilized: ≤30℃ Madaw |
| Nolosha shelf | Dareeraha: 9 bilood;Lyophilized: 12 bilood |
| Nooca Tuunada | unug cusub oo buro ah, qayb barafaysan, unug cudur-sidaha paraffin-ku-xidhan ama qayb, balaasma ama serum |
| CV | 5.0% |
| LoD | Ogaanshaha xalalka falcelinta aashitada nucleic ee hoosta asalka 3ng/μL nooca duurjoogta ah, waxay si adag u ogaan kartaa heerka isbeddelka 1% |
| Gaar ahaan | Ma jiro falcelin iskutallaab ah oo leh DNA-ga aadanaha nooca duurjoogta ah iyo noocyada kale ee mutant |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ahNidaamyada noolaha ee la dabaqay 7300 Nidaamyada PCR-waqtiga-dhabta ah QuantStudio® 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler® 480 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
