Enterovirus Universal
Magaca alaabta
HWTS-EV001-Enterovirus Qalabka Ogaanshaha Ashitada Nukliyeerka Caalamiga ah (Fluorescence PCR)
Epidemiology
Cudurka afka-gacmeedku waa cudur la is qaadsiiyo oo ay keento enteroviruses (EV).Hadda, 108 nooc oo serotypes of enteroviruses ah ayaa la helay, kuwaas oo loo qaybiyay afar kooxood: A, B, C iyo D. Waxaa ka mid ah, enterovirus EV71 iyo CoxA16 waa cudur-sidaha ugu muhiimsan.Cudurku wuxuu inta badan ku dhacaa carruurta da'doodu ka yar tahay 5 sano, wuxuuna ku keeni karaa herpes gacmaha, cagaha, afka iyo qaybaha kale.Tiro yar oo carruur ah ayaa ku dhici doona dhibaatooyin sida myocarditis, bararka sambabada, iyo meningoencephalitis aseptic.
Kanaalka
| FAM | EV RNA |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Oropharyngeal suuf,Dheecaan Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/ml |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500/7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Qalabka Soo-saarida ee lagu talinayo: Macro & Micro-Tijaabada Guud ee DNA/RNA Qalabka (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) HWTS-3006B, HWTS-3006C), waa in si adag loo soo saaro si waafaqsan tilmaamaha.Mugga muunada waa 200 μL, mugga sifaynta lagu taliyey waa 80µL.
Doorashada 2.
Xirmada soo saarista lagu taliyay: Macro & Micro-Test Tusaalaha Siideynta Reagent (HWTS-3005-8), waa in si adag loo soo saaro si waafaqsan tilmaamaha.
Ikhtiyaarka3.
Xirmada soo saarista ee lagu taliyay: QIAamp Viral RNA Mini Kit (52904) ama Soosaarka Asiidhka Nucleic ama Qalabka Sifaynta (YDP315-R), waa in si adag loo soo saaro si waafaqsan tilmaamaha.Mugga muunada waa 140 μL, mugga sifaynta lagu taliyey waa 60µL.
