La qaboojiyey Zaire iyo Suudaan Ebola Virus Nucleic Acid
Magaca alaabta
HWTS-FE035-Baraf-qallalan Zaire iyo Suudaan Qalabka Lagu Ogaado Fayraska Ebola Nucleic Acid (Fluorescence PCR)
Epidemiology
Fayraska Ebola waxaa iska leh Filoviridae, oo ah fayraska RNA-xun ee taban oo aan kala qaybsanayn. Fayrasyadu waa fiilooyin dhaadheer oo celcelis ahaan dhererkoodu yahay 1000nm dhexroorkiisuna yahay 100nm. Cudurka Ebolavirus genome waa RNA aan fiicneyn oo taban oo leh cabbir dhan 18.9kb, oo dejinaya 7 borotiinno qaab-dhismeed ah iyo 1 borotiin aan dhismo ahayn. Fayraska Ebola waxa loo qaybin karaa noocyo ay ka mid yihiin Zaire, Sudan, Bundibugyo, Tai Forest iyo Reston. Waxaa ka mid ah, nooca Zaire iyo nooca Sudan ayaa la sheegay inay sababeen dad badan inay u dhintaan caabuqa. EHF (Ebola Hemorrhagic Fever) waa cudur faafa oo dhiigbax ah oo ba'an oo uu keeno fayraska Ebola. Bani'aadamka waxaa inta badan ku dhaca taabashada dareeraha jirka, dheecaannada iyo saxarada bukaannada ama xayawaanka qaba cudurka, calaamadaha bukaan-socodka ayaa inta badan ah qandho soo baxda, dhiigbax iyo dhaawac badan oo xubno ah. EHF waxay leedahay heerka dhimashada sare ee 50%-90%. Waqtigan xaadirka ah, hababka ogaanshaha fayraska Ebola waxay u badan yihiin baaritaannada shaybaarka, oo ay ku jiraan laba arrimood: ogaanshaha etiological iyo ogaanshaha serological. Ogaanshaha Etiological waxaa ka mid ah ogaanshaha antigen-ka fayraska ee shaybaarada dhiigga ee ELISA, lagu ogaanayo asiidhka nucleicka iyadoo la adeegsanayo hababka kor u qaadista sida RT-PCR, iwm, iyo isticmaalka unugyada Vero, Hela, iwm. si loo go'doomiyo fayraska iyo dhaqanka. Ogaanshaha serological waxaa ka mid ah ogaanshaha Serum gaar ah unugyada difaaca jirka ee IgM iyadoo la qabanayo ELISA, iyo ogaanshaha Serum gaar ah unugyada difaaca IgG ee ELISA, immunofluorescence, iwm.
Qiyaasaha Farsamada
Kaydinta | ≤30℃ |
Nolosha shelf | 12 bilood |
Nooca Tuunada | serum, plamuunado sma |
CV | ≤5.0% |
LoD | 500 koobi/μL |
Qalabka lagu dabaqi karo | Lagu dabaqi karo nooca I ogaanshaha reagent: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), Nidaamyada ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer), MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerarka Heerarka (Suzhou Molarray Co., Ltd.), BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah. Lagu dabaqi karo nooca II reagent ogaanshaha: EudemonTMAIO800 (HWTS-EQ007) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Tijaabada Toomaatiga ah ee Acid Nucleic Acid (HWTS-3006). Soo saarista waa in loo qabtaa si waafaqsan tilmaamaha, mugga muunada la soo saarayna waa 200μL iyo mugga sare ee lagu taliyey waa 80μL.