Qalabka Ogaanshaha Aashitada Nucleic-ga ee Gardnerella Vaginalis (PCR-ga Fluorescence)
Magaca alaabta
Qalabka Ogaanshaha Aashitada Nucleic-ga ee Gardnerella Vaginalis (PCR-ga Fluorescence)
Cudurrada Faafa
Sababta ugu badan ee keenta vaginitis-ka dumarka waa bakteeriyada vaginosis, bakteeriyada muhiimka ah ee bakteeriyada vaginosis-kana waa Gardnerella vaginalis. Gardnerella vaginalis (GV) waa cudur faafa oo aan keenin cudur marka ay tiro yar ku jiraan. Si kastaba ha ahaatee, marka bakteeriyada ugu badan ee siilka ee Lactobacilli la yareeyo ama la baabi'iyo, taasoo keenta dheelitir la'aan ku timaada deegaanka siilka, Gardnerella vaginalis waxay ku tarmaan tiro badan, taasoo keenta vaginosis-ka bakteeriyada. Isla mar ahaantaana, cudur faafa oo kale (sida Candida, Neisseria gonorrhoeae, Chlamydia trachomatis, iwm.) waxay u badan tahay inay soo galaan jirka aadanaha, taasoo keenta vaginitis isku dhafan iyo cervicitis. Haddii vaginitis iyo cervicitis aan la ogaan oo aan si waqtiyeysan oo wax ku ool ah loo daaweyn, waxaa jiri kara caabuqyo ay keenaan jeermisyada ku dhaca xuubka taranka, taasoo si fudud u horseedi karta caabuqyada sare ee taranka sida endometritis, salpingitis, tubo-ovarian abscess (TOA), iyo peritonitis miskaha, taasoo keeni karta dhibaatooyin halis ah sida dhalmo la'aan, uurka ectopic iyo xitaa natiijooyinka uurka ee xun.
Halbeegyada Farsamada
| Kaydinta | -18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | suufka kaadi mareenka ragga, suufka afka ilmo-galeenka dumarka, suufka siilka dumarka |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 400 Nuqul/mL |
| Qalabka Khuseeya | Waxaa lagu dabaqi karaa nooca I ee wax soo saarka ogaanshaha:Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah, QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah, Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.), Nidaamyada Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A, tignoolajiyada Hangzhou Bioer), Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.), Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX96, Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96. Waxaa lagu dabaqi karaa nooca II ee wax soo saarka ogaanshaha: EudemonTMAIO800 (HWTS-EQ007) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
Wakiilka Reagent-ka waa loo baahan yahay laakiin lama bixin
Qalabka DNA/RNA ee caadiga ah ee saline-ka, Macro & Micro-Test-ka Guud (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Alaabta la isticmaalo waa loo baahan yahay laakiin lama bixin
Talooyin aan RNase/DNase lahayn, galoofisyo la tuuri karo, tuubooyin aan RNase/DNase lahayn, centrifuge, magnetic stand, 8-tube strips oo loogu talagalay PCR.
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