HCV Genotyping
Magaca Alaabta
HWTS-HP004-HCV Xirmada Garashada Genotyping (Fluorescence PCR)
Epidemiology
Fayraska cagaarshow C (HCV) waxaa iska leh qoyska flaviviridae, genome-kiisuna waa hal xarig oo togan RNA, kaas oo si sahal ah isu beddelay.Fayrasku wuxuu ku jiraa hepatocytes, serum leukocytes iyo balasmaha dadka cudurka qaba.Hidde-sidayaasha HCV waxay u nugul yihiin isbeddelka waxaana loo qaybin karaa ugu yaraan 6 genotypes iyo noocyo hoose oo badan.Noocyada kala duwan ee HCV genotypes waxay isticmaalaan DAAs daawaynta kala duwan iyo koorsooyinka daawaynta.Sidaa darteed, ka hor inta aan bukaannada lagu daweyn DAA antiviral therapy, HCV genotype waa in la ogaadaa, xitaa bukaanada qaba nooca 1, waa lagama maarmaan in la kala saaro haddii ay tahay nooca 1a ama nooca 1b.
Kanaalka
| FAM | Nooca 1b, Nooca 2a |
| ROX | Nooca 6a, Nooca 3a |
| VIC/HEX | Xakamaynta Gudaha, Nooca 3b |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ Madow |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Serum, Plasma |
| Ct | ≤36 |
| CV | ≤5.0 |
| LoD | 200 IU/ml |
| Gaar ahaan | Isticmaal qalabkan si aad u ogaato fayraska kale ama sambalyada bakteeriyada sida: cytomegalovirus aadanaha, fayraska Epstein-Barr, fayraska difaaca jirka, fayraska cagaarshow B, fayraska cagaarshow A, waraabowga, fayraska herpes nooca 6, fayraska herpes simplex nooca 1, simplex fayraska Herpes. nooca 2, fayraska hargabka A, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, iwm. Natiijooyinka dhamaantood waa taban. |
| Qalabka lagu dabaqi karo | Waxay la jaan qaadi kartaa qalabka PCR-ka ee caadiga ah ee suuqa. ABI 7500 Nidaamyada PCR-waqtiga-dhabta ah ABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
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