Bini-aadamka Cyp2c9 iyo VKORC1 HIT POLY lystrorphism
Magaca wax soo saarka
HWTS-GUT0 -14A-Aadanaha Cyp2C9 iyo VKorc1 Genommorphism Kicil (PCR)
Shahaado
CE / TFDA
Nadiifin
Warfarin waa anticouglan afka ah oo sida caadiga ah loo isticmaalo waxqabadka rugta caafimaad ee hadda, oo inta badan loogu talagalay ka-hortagga iyo daaweynta cudurada trainbomal-ka. Si kastaba ha noqotee, Warfarin wuxuu leeyahay daaqad daaweyn oo xadidan oo aad ugu kala duwan tahay jinsiyada iyo shaqsiyaadka kala duwan. Tirakoobyadu waxay muujiyeen in farqiga u dhexeeya qiyaasta qiyaasta xasilloonida ee shaqsiyaadka kaladuwan ay ka badnaan karaan 20 jeer. Dhiig-baxa xun ee xun wuxuu ku dhacaa 15.2% bukaannada qaadanaya xaffarin sanad walba, kaas oo 3.5% ay yeeshaan dhiigbaxa dilaaga ah. Daraasadaha dawooyinka lagu daray ee dawooyinka loo yaqaan 'payrogenomic' ayaa muujisay in hidde-wadaha 'plstymyrphism' ee bartilmaameedka Enzorc1 iyo dheef-shiid kiimikaadka VKzyme Cyp2C9 ee Warfarin waa qodob muhiim u ah farqiga u dhexeeya qaddarka Warfarin. Warfarin waa xakameyn gaar ah oo ka hortaga dhimirka fitamiin Kefamiin ee fipoxide (VKORC1), sidaas darteedna waxay xannibayaan isku-darka akoonku oo ay kujirto fitamiin K oo ay siiyaan ku rakiban. Daraasado badan ayaa muujisay in hidde-siinta 'polymorphisprim-ka' VKorc1 'waa qodobka ugu muhiimsan ee saameynaya midabka iyo kala duwanaanta shaqsiyadeed ee ah qadka Warfarin ee loo baahan yahay. Warfarin waxaa qaada Cyp2c9, iyo istaroolkoodii aad u jajaban dheef-shiid kiimikaad ee Warfarin. Shakhsiyaadka isticmaalaya Warfarin waxay leeyihiin halis sare (laba jeer ilaa saddex jeer ka sareeya) dhiig-baxa marxaladda hore ee isticmaalka.
Kanaal
| Fa farqad | Vkorc1 (-1639g> a) |
| Cy5 | Cyp2c9 * 3 |
| Vic / hex | IC |
Cayayaanka farsamada
| Bakhaar | Dareere: ≤-18 ℃ |
| Shelf-Life | 12 bilood |
| Nooca muunadda | Edta oo cusub edta lidka ku ah daawada |
| CV | ≤5.0% |
| Dhigid | 1.0ng / μL |
| Si gaar ah | Ma jiro wax-ka-qabasho iskutallaab ah oo ay la socdaan taxanaha kale ee isdaba joog ah ee aadanaha (bini-aadamka Cyp2c1c1c1c1c1C1) hidda-wadaha, hiddo-wadaha aadanaha RPN2; isbedelka Cyp2c9 * 13 iyo VKORC1 (3730g> a) ka baxsan meesha laga ogaado xirmadan |
| Aaladaha lagu dabaqi karo | Waxaa lagu dabaqay biosystemy-ga ee lagu dabaqay 7500 nidaamyada waqtiga-dhabta ah ee PCR Waxaa lagu dabaqay bio-yariyada bio-yada lagu dabaqay 7500 oo si dhakhso leh u ah nidaamyada PCR-ga Jadwalka PCRSSDUDDUDO®5 Nidaamyada PCR Nidaamyada 'Slacan-96p' ee waqtiga dhabta ah ee PCR Nalalka Firfircoon480 Nidaamka PCR-ka waqtiga-dhabta ah Sadarka 9600 oo lagu daray nidaamka ogaanshaha PCR-ka waqtiga-dhabta ah MA-6000 baaskiil-ku-meel-gaadhka waqtiga-ku-meel-gaarka ah Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Biorad Cfx Opus 96 Nidaamka PCR-ka waqtiga-dhabta ah |
Socodka shaqada
Soosaarista lagu taliyay Reaggent: Macro-Imtixaanka Micro-Micro-Micro-Imtixaanka fayraska DNA / HWTS-3001, HWTS-3004-38, HWTS-3004-96) iyo macro-maqaaxida otomatiga nucro ah (hwts- 3006).
