Isbeddelka Hidde-sidaha ee CYP2C9 ee Aadanaha iyo VKORC1
Magaca alaabta
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-GE014A-Human CYP2C9 iyo VKORC1 (Fluorescence PCR)
Shahaadada
CE/TFDA
Cudurrada Faafa
Warfarin waa daawo afka laga qaato oo loo isticmaalo daaweynta xinjirowga dhiigga, taasoo inta badan loogu talagalay ka hortagga iyo daaweynta cudurrada xinjirowga dhiigga. Si kastaba ha ahaatee, warfarin wuxuu leeyahay daaqad daaweyn oo xaddidan wuxuuna si weyn ugu kala duwan yahay jinsiyadaha iyo shakhsiyaadka kala duwan. Tirakoobyadu waxay muujiyeen in farqiga qiyaasta deggan ee shakhsiyaadka kala duwan uu noqon karo wax ka badan 20 jeer. Dhiigbaxa falcelinta xun wuxuu dhacaa 15.2% bukaanada qaata warfarin sannad kasta, kuwaas oo 3.5% ay yeeshaan dhiigbax dilaa ah. Daraasadaha dawooyinka ayaa muujiyay in isbeddelka hidde-side ee enzyme-ka bartilmaameedka ah ee VKORC1 iyo enzyme-ka dheef-shiid kiimikaadka CYP2C9 ee warfarin uu yahay arrin muhiim ah oo saameynaysa farqiga qiyaasta warfarin. Warfarin waa daawo gaar ah oo ka hortagta fitamiin K epoxide reductase (VKORC1), sidaas darteedna waxay joojisaa isku-darka xinjirowga ee ku lug leh fitamiin K waxayna bixisaa xinjirowga dhiigga. Tiro badan oo daraasado ah ayaa muujiyay in isbeddelka hidde-side ee kor u qaadaha VKORC1 uu yahay qodobka ugu muhiimsan ee saameeya kala duwanaanshaha jinsiyadda iyo shakhsiyaadka ee qiyaasta loo baahan yahay ee warfarin. Warfarin waxaa dheefshiida CYP2C9, mutant-kiisuna aad ayuu u gaabiyaa dheef-shiid kiimikaadka warfarin. Dadka isticmaala warfarin waxay leeyihiin khatar sare (laba jeer ilaa saddex jeer ka badan) oo dhiigbax ah marxaladda hore ee isticmaalka.
Kanaal
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Halbeegyada Farsamada
| Kaydinta | Dareere: ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Dhiig cusub oo xinjirowga lidka ku ah EDTA |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Gaar ahaanshaha | Ma jiro fal-celin isdhaafsi ah oo leh taxane kale oo aad isugu xiran oo hiddo-sidayaasha aadanaha ah (hiddaha CYP2C19 ee aadanaha, hiddo-sidayaasha RPN2 ee aadanaha); isbeddelka CYP2C9*13 iyo VKORC1 (3730G>A) oo ka baxsan baaxadda ogaanshaha ee xirmadan |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: Qalabka DNA/RNA ee Macro & Micro-Test Viral (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
