Bani'aadamka PML-RARA Isku-dhafka Hiddaha
Magaca alaabta
HWTS-TM017AQalabka ogaanshaha isku-dhafan ee PML-RARA Fusion ee Aadanaha (Fluorescence PCR)
Epidemiology
Ba'an promyelocytic leukemia (APL) waa nooc gaar ah oo ah leukemia myeloid ba'an (AML). Qiyaastii 95% bukaannada APL waxaa weheliya isbeddel gaar ah oo cytogenetic ah, kuwaas oo ah t(15;17)(q22;q21), taas oo ka dhigaysa hidda-wadaha PML ee koromosoomka 15 iyo reseptor-ka retinoic acid α hidda (RARA) ee koromosoomka 17 oo isku dhafan si loo sameeyo hidda-wadaha PML-RARA. Sababo la xiriira meelo kala duwan oo hiddo-wadaha PML-ka ah, hidde-wadaha isku-dhafka PML-RARA waxa loo qaybin karaa nooc dheer (nooc L), nooc gaaban (nooca S) iyo nooc ka duwan (nooca V), oo xisaabinaya qiyaastii 55%, 40% iyo 5% siday u kala horreeyaan.
Kanaalka
| FAM | Hiddaha isku-dhafka PML-RARA |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ Madow |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | dhuuxa lafta |
| CV | <5.0 |
| LoD | 1000 koobi/ml |
| Gaar ahaan | Ma jiro falcelin iskutallaab ah oo leh hiddo-wadaha isku-dhafka kale ee BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, iyo TEL-AML1 hiddo-wadaha isku-dhafka |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: RNAprep Wadarta Dhiiga Saafiga ah ee Xidhmada Soo Saarista RNA (DP433). Soo saarista waa in loo qabtaa si waafaqsan IFU.
