Isbeddelka Hidde-sidaha Isku-dhafka ah ee Aadanaha PML-RARA
Magaca alaabta
HWTS-TM017AQalabka Ogaanshada Isbeddelka Hidde-sidaha ee Aadanaha PML-RARA (Fluorescence PCR)
Cudurrada Faafa
Cudurka 'acute promyelocytic leukemia' (APL) waa nooc gaar ah oo ah cudurka 'myeloid leukemia' (AML). Qiyaastii 95% bukaannada APL waxaa la socda isbeddel gaar ah oo ku dhaca unugyada dhiigga, kaas oo ah t(15;17)(q22;q21), kaas oo ka dhigaya hidda-wadaha PML ee koromosoomka 15 iyo hidda-wadaha 'retinoic acid receptor α' (RARA) ee koromosoomka 17 inay isku milmaan si ay u sameeyaan hidda-wadaha 'PML-RARA'. Sababtoo ah meelaha kala duwan ee jabka hidda-wadaha PML, hidda-wadaha 'PML-RARA' waxaa loo qaybin karaa nooca dheer (nooca L), nooca gaaban (nooca S) iyo nooca kala duwan (nooca V), oo u dhigma qiyaastii 55%, 40% iyo 5% siday u kala horreeyaan.
Kanaal
| FAM | Hidde-sidaha isku-dhafka PML-RARA |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ Mugdi ku jira |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | dhuuxa lafaha |
| CV | <5.0％ |
| LoD | 1000 Nuqul/mL. |
| Gaar ahaanshaha | Ma jiro wax fal-celin ah oo isdhaafsi ah oo lala sameeyo hiddo-sidayaasha kale ee isku-dhafka ah ee BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, iyo hiddo-sidayaasha isku-dhafka ah ee TEL-AML1. |
| Qalabka Khuseeya | Nidaamka PCR-ga ee Waqtiga-dhabta ah ee La Adeegsaday ee Biosystems 7500 Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A, tignoolajiyada Hangzhou Bioer) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: RNAprep Pure Blood Total RNA Extracting Kit (DP433). Soo saarista waa in loo sameeyaa iyadoo loo eegayo IFU.
