Isbeddelka Hidde-sidaha Isku-dhafka Aadanaha ee TEL-AML1
Magaca alaabta
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-TM016 ee Aadanaha TEL-AML1 (Fluorescence PCR)
Cudurrada Faafa
Leukemia-ga lymphoblastic-ga daran (ALL) waa cudurka ugu badan ee carruurta. Sannadihii ugu dambeeyay, leukemia-ga daran (AL) ayaa ka beddeshay nooca MIC (qaab-dhismeedka, difaaca jirka, cytogenetics) una beddeshay nooca MICM (ku darista baaritaanka bayoolajiga molecular-ka). Sannadkii 1994, waxaa la ogaaday in isku-darka TEL ee carruurnimada uu sababay isbeddelka koromosoomka aan kala sooca lahayn t(12;21)(p13;q22) ee leukemia-ga daran ee lymphoblastic-ga ee B-lineage (ALL). Tan iyo markii la helay hidda-wadaha isku-darka AML1, hidda-wadaha isku-darka TEL-AML1 waa habka ugu wanaagsan ee lagu qiimeeyo saadaasha carruurta qabta leukemia-ga daran ee lymphoblastic.
Kanaal
| FAM | Hidde-sidaha isku-dhafka TEL-AML1 |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | muunad dhuuxa lafta ah |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000 Nuqullo/mL |
| Gaar ahaanshaha | Ma jiro wax fal-celin ah oo u dhexeeya qalabka iyo hiddo-sidayaasha kale ee isku-dhafka sida hiddo-sidayaasha isku-dhafka BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, iyo hiddo-sidayaasha isku-dhafka PML-RARa. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Qalabka Soo Saarista RNA ee RNAprep ee Total Blood Pure (DP433).
