Bani'aadamka TEL-AML1 Isku-beddelka Hiddaha
Magaca alaabta
HWTS-TM016 Bani'aadamka TEL-AML1 Qalabka Ogaanshaha Isku-beddelka Hiddaha (Fluorescence PCR)
Epidemiology
Leukemia ba'an ee lymphoblastic (ALL) waa malignantiga ugu badan ee caruurnimada.Sannadihii ugu dambeeyay, kansarka ba'an ee leukemia (AL) ayaa ka beddelay nooca MIC (morphology, immunology, cytogenetics) oo noqday nooca MICM (oo lagu daray baaritaanka bayoolajiga molecular).1994-kii, waxaa la ogaaday in isku-dhafka TEL ee caruurnimada uu sababay translocation koromosoomyada aan random-ka ahayn t(12;21)(p13;q22) ee B-lineage acute lymphoblastic leukemia (ALL).Tan iyo markii la helay hiddo-wadaha isku-dhafka AML1, hiddo-wadaha isku-dhafka TEL-AML1 waa habka ugu wanaagsan ee lagu xukumi karo saadaasha carruurta qaba leukemia ba'an.
Kanaalka
| FAM | TEL-AML1 hidda-wadaha isku dhafka ah |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | muunadda dhuuxa lafta |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000 koobi/ml |
| Gaar ahaan | Ma jiro wax falcelin ah oo u dhexeeya xirmooyinka iyo hiddo-wadaha kale ee fiyuuska sida BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, hiddo-wadaha isku-dhafka PML-RARa. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
RNAprep Wadarta Dhiiga Saafiga ah ee Xidhmada Soo Saarista RNA (DP433).
