Hargabka A/B
Magaca alaabta
HWTS-RT003A Hargabka A/B Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Hargabka A waa cudur faafa oo neef-mareenka oo ba'an, oo leh noocyo badan oo hoosaadyo ah sida H1N1 iyo H3N2, kuwaas oo u nugul isbeddelka oo ka dillaacay adduunka oo dhan.Isbeddelka antigenic waxa loola jeedaa isbeddelka fayraska hargabka A, taasoo keentay soo bixitaanka nooc cusub oo cusub.Fayrasyada hargabka B waxay u qaybsan yihiin laba qaybood oo waaweyn, Yamagata iyo Victoria.Fayrasyada hargabka B waxay leeyihiin oo kaliya qulqulka antigenic, waxayna ka baxsadaan ilaalinta iyo nadiifinta nidaamka difaaca bini'aadamka iyada oo loo marayo isbeddelkooda.Si kastaba ha ahaatee, heerka kobaca ee fayraska hargabka B wuu ka gaabiyaa fayraska hargabka A, fayraska hargabka B wuxuu kaloo keeni karaa caabuqa habdhiska neefsiga ee bini'aadamka wuxuuna u horseedi karaa cuduro faafa.
Kanaalka
| FAM | IFV A |
| ROX | Xakamaynta Gudaha |
| VIC/HEX | IFV B |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | suufka oropharyngeal |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A: 500 koobi/ml, IFV B: 500 koobi/ml |
| Gaar ahaan | 1. Falcelinta isdhaafsiga: ma jiro falcelin iskutallaab ah oo udhaxeysa xirmadan iyo nooca adenovirus nooca 3, 7, coronavirus human SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, iyo HCoV-NL63, cytomegalovirus, caabuqa enterovirus, fayraska parainfluenza, fayraska jadeecada, fayraska metapneumovirus, fayraska qaamo-qashiirta, fayraska syncytial neef-mareenka nooca B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacularlenthabacteria myopia, pneumonia, pneumonia. e, neisseria meningitidis . 2. Tijaabada faragelinta: Walxaha faragelinta waxaa loo doortaa sida mucin (60mg/mL), dhiiga dadka, oxymetazoline (2mg/mL), baaruud (10%), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide ( 20μg/ml), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL). ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/ml), oseltamivir phosphate (60ng/ml), ribavirin (10mg/L), iyo natiijadu waxay muujinaysaa in walxaha faragelinta leh ee kor ku xusan aysan lahayn falcelin faragelin ah oo ku saabsan ogaanshaha qalabka. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah iyo BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Dib-u-hagaajinta soo saarista lagu taliyay: Makro & Micro-Tijaabada Guud ee DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (kaaso loo isticmaali karo Macro & Micro-Test Soo saarista Ashitada Nukliyeerka Tooska ah (HWTS-3006C, HWTS-3006B)) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in loo qabtaa si waafaqsan IFU.Mugga muunadda soo saariddu waa200μL.Mugga sifaynta lagu taliyey waa 80μL.
Doorashada 2.
Dib-u-hagaajinta soo saarista ee lagu taliyay: Makro & Tijaabada Muunadda Siideynta Reagent (HWTS-3005-8).Soo saarista waa in loo qabtaa si waafaqsan IFU.
Doorashada 3.
Soo saarista lagu taliyay: Soosaarka Asiidhka Nucleic ama Qalabka Nadiifinta (YDP315-R).Soo saarista waa in loo fuliyaa si waafaqsan IFU.Mugga muunada soo saarista waa 140μL.Mugga sare ee lagu taliyey waa 60μL.
