Mycobacterium Tuberculosis INH Mutation
Magaca alaabta
HWTS-RT137 Mycobacterium Tuberculosis INH Qalooca Wax-ka-beddelka
Epidemiology
Mycobacterium tuberculosis, wax yar sida Tubercle Bacillus (TB), waa bakteeriyada keenta cudurka qaaxada. Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka kowaad ee ka hortagga qaaxada waxaa ka mid ah INH, rifampicin iyo hexambutol, iwm. Dawooyinka labaad ee ka hortagga qaaxada waxaa ka mid ah fluoroquinolones, amikacin iyo kanamycin, iwm. Daawooyinka cusub ee la sameeyay ayaa kala ah linezolid, bedaquiline iyo delamani, iwm. Hase yeeshee, isticmaalka khaldan ee daawooyinka qaaxada ee unugyada unugyada bakteeriyada mycobacterium tuberculosis waxay samaysaa caabbinta daawada ee daawooyinka ka hortagga tiibishada, taas oo keeneysa caqabado halis ah ka hortagga iyo daaweynta tiibishada.
Kanaalka
| FAM | MP nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | xaako |
| CV | ≤5% |
| LoD | Xadka lagu ogaanayo bakteeriyada nooca duurjoogta ah ee INH waa 2x103 bakteeriya/mL, iyo xadka ogaanshaha bakteeriyada mutant waa 2x103 bakteeriyada/mL. |
| Gaar ahaan | a. Ma jiraan wax falcelin ah oo ka dhexeeya genome-ka bini'aadamka, mycobacteria kale ee aan tuberkule ahayn iyo cudur-sidaha oof-wareen ee uu qalabkani ogaado.b. Goobaha beddelka ee hiddo-wadaha kale ee u adkaysta dawada ee qaaxada Mycobacterium ee duurjoogta ah, sida caabbinta go'aamisa gobolka hidda-wadaha rifampicin rpoB, ayaa la ogaaday, natiijadii baadhistuna ma muujin wax iska caabin ah INH, taas oo muujinaysa in aanay jirin falcelin iskutallaab ah. |
| Qalabka lagu dabaqi karo | SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ahBioRad CFX96 Nidaamyada PCR-Waqtiga-dhabta ahLightCycler480®Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Haddii aad isticmaasho Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. in lagu tijaabiyo muunad ka mid ah 2. isku xigxiga, oo ku dar 10μL kontoroolka gudaha si gooni gooni ah kantaroolka taban, muunada xaakada ee la farsameeyay si loo tijaabiyo, talaabooyinka xiga waa in si adag loo fuliyaa si waafaqsan tilmaamaha soo saarista. Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 100μL.
