Isbeddelka Mycobacterium Tuberculosis INH
Magaca alaabta
Qalabka Ogaanshada Isbeddelka ee HWTS-RT137 Mycobacterium Tuberculosis INH (Qalooca Dhalaalka)
Cudurrada Faafa
Qaaxada Mycobacterium, oo ah nooca Tubercle Bacillus (TB), waa bakteeriyada keenta cudurka qaaxada. Waqtigan xaadirka ah, daawooyinka ka hortagga qaaxada ee safka hore ee loo isticmaalo waxaa ka mid ah INH, rifampicin iyo hexambutol, iwm. Daawooyinka ka hortagga qaaxada ee safka labaad waxaa ka mid ah fluoroquinolones, amikacin iyo kanamycin, iwm. Daawooyinka cusub ee la sameeyay waa linezolid, bedaquiline iyo delamani, iwm. Si kastaba ha ahaatee, sababtoo ah isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo astaamaha qaab-dhismeedka derbiga unugyada ee qaaxada mycobacterium, qaaxada mycobacterium waxay horumarisaa iska caabbinta daawada ee daawooyinka ka hortagga qaaxada, taas oo keenta caqabado halis ah oo ku yimaada ka hortagga iyo daaweynta qaaxada.
Kanaal
| FAM | Aashitada nukliyeerka MP |
| ROX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | xaako |
| CV | ≤5% |
| LoD | Xadka ogaanshaha bakteeriyada INH ee nooca duurjoogta ah waa 2x103 bakteeriya/mL, xadka ogaanshaha bakteeriyada isbeddelka lehna waa 2x103 bakteeriya/mL. |
| Gaar ahaanshaha | a. Ma jiro wax falcelin isdhaafsi ah oo ka dhex dhaca hiddo-wadaha aadanaha, bakteeriyada kale ee aan qaaxada lahayn iyo jeermisyada oof-wareenka ee uu qalabkani ogaado.b. Goobaha isbeddelka ee hiddo-sideyaasha kale ee daawada u adkaysta ee qaaxada Mycobacterium ee nooca duurjoogta ah, sida gobolka go'aamiya iska caabbinta ee hidda-wadaha rifampicin rpoB, ayaa la ogaaday, natiijooyinka baaritaankuna waxay muujiyeen inaysan jirin wax iska caabbin ah oo loo geysto INH, taasoo muujinaysa inaysan jirin wax falcelin isdhaafsi ah. |
| Qalabka Khuseeya | Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96PNidaamyada PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96LightCycler480®Nidaamka PCR-ka Waqtiga-dhabta ah |
Socodka Shaqada
Haddii aad isticmaasho Qalabka Guud ee Macro & Micro-Test DNA/RNA (HWTS-3019) (oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. si aad u soo saarto, ku dar 200μL oo ah muunadda xakamaynta taban iyo xaakada la farsameeyay si loogu tijaabiyo si isku xigta, oo ku dar 10μL oo ah xakamaynta gudaha si gaar ah muunadda xakamaynta taban, xaakada la farsameeyay si loo tijaabiyo, tallaabooyinka xigana waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha soo saarista. Mugga muunadda la soo saaray waa 200μL, mugga dheecaanka lagu taliyayna waa 100μL.
