Mycobacterium Qaaxada Isoniazid Resistance Mutation

Mycobacterium Tuberculosis Isoniazid Is-beddelka Iska-caabbinta Sawirka

Sharaxaad Gaaban:

Qalabkani wuxuu ku haboon yahay in lagu ogaado tayada goobaha ugu muhiimsan ee isku-beddelka ee shaybaarada xaakada bani'aadamka ee laga soo ururiyay bukaanada Tubercle bacillus positive ah taas oo u horseedda mycobacterium tuberculosis isoniazid caabbinta: Gobolka dhiirigeliyaha InhA -15C>T, -8T>A, -8T>C;Gobolka horumariyaha AhpC -12C>T, -6G>A;isu beddelka qaniisyada ee KatG 315 codon 315G>A, 315G>C .

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Magaca alaabta

HWTS-RT137 Mycobacterium Qaaxada Isoniazid Qalabka Wax-ka-Beddelka Iska-caabbinta (qallooca dhalaalay)

Epidemiology

Mycobacterium tuberculosis, wax yar sida Tubercle Bacillus (TB), waa bakteeriyada keenta cudurka qaaxada.Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka koowaad ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo hexambutol, iwm. Si kastaba ha ahaatee, isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo sifooyinka qaab dhismeedka gidaarka unugyada qaaxada mycobacterium, qaaxada mycobacterium waxay samaysaa caabbinta daawada daawooyinka ka hortagga qaaxada, taas oo keeneysa caqabado halis ah ka hortagga iyo daaweynta qaaxada.

Kanaalka

FAM	MP nucleic acid
ROX	Xakamaynta Gudaha

Qiyaasaha Farsamada

Kaydinta	≤-18℃
Nolosha shelf	12 bilood
Nooca Tuunada	xaako
CV	≤5%
LoD	Xadka lagu ogaanayo bakteeriyada isoniazid ee u adkaysata nooca duurjoogta ah waa 2x103 bakteeriya/mL, iyo xadka ogaanshaha bakteeriyada mutant waa 2x103 bakteeriyada/ml.
Gaar ahaan	a.Ma jiraan wax falcelin ah oo ka dhexeeya genome-ka bini'aadamka, mycobacteria kale ee aan tuberkule ahayn iyo cudur-sidaha oof-wareen ee uu qalabkani ogaado.b.Goobaha beddelka ee hiddo-wadaha kale ee u adkaysta dawada ee qaaxada Mycobacterium ee duurjoogta ah, sida caabbinta go'aamisa gobolka hidda-wadaha rifampicin rpoB, ayaa la ogaaday, natiijadii baadhistana ma muujin wax iska caabin ah oo isoniazid ah, taas oo muujinaysa in aanay jirin fal-celin iskutallaab ah.
Qalabka lagu dabaqi karo	SLAN-96P Nidaamyada PCR-waqtiga-dhabta ahBioRad CFX96 Nidaamyada PCR-Waqtiga-dhabta ah LightCycler480® Nidaamka PCR-waqtiga-dhabta ah

Socodka Shaqada

Haddii la isticmaalo Makro & Micro-Test DNA / RNA Kit (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. si loo soo saaro, ku dar 200μL ee xakamaynta taban iyo muunada xaakada ee la farsameeyay si si isku xigta loo tijaabiyo, kuna dar 10μL ee kontoroolka gudaha si gooni gooni ah u geliyo kantaroolka taban, muunada xaakada ee la farsameeyay si loo tijaabiyo, talaabooyinka xiga waa in si adag loo fuliyaa si waafaqsan tilmaamaha soo saarista.Mugga muunadda la soo saaray waa 200μL, iyo mugga caddaynta lagu taliyey waa 100μL.