Mycobacterium tuberculosis Nucleic Acid iyo Rifampicin Resistance
Magaca alaabta
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin Resistance Resistance Kit (qallooca dhalaalay)
Shahaado
CE
Epidemiology
Mycobacterium tuberculosis, wax yar sida Qaaxada Bacillus, TB, waa bakteeriyada keenta cudurka qaaxada.Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka koowaad ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo hexambutol, iwm. Si kastaba ha ahaatee, isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo sifooyinka qaab dhismeedka gidaarka unugyada qaaxada mycobacterium, qaaxada mycobacterium waxay samaysaa caabbinta daawada daawooyinka ka hortagga qaaxada, taas oo keeneysa caqabado halis ah ka hortagga iyo daaweynta qaaxada.
Rifampicin ayaa si weyn loogu isticmaalay daawaynta bukaanada qaaxada sambabada tan iyo dabayaaqadii 1970-meeyadii, waxayna leedahay saameyn weyn.Waxay ahayd doorashada koowaad ee lagu soo gaabiyo daaweynta kemotherabi ee bukaannada qaaxada sambabada.Iska caabbinta Rifampicin waxaa badanaa sababa isbeddelka hidda-wadaha rpoB.Inkasta oo dawooyinka cusub ee ka hortagga qaaxada ay si joogto ah u soo baxayaan, iyo waxtarka bukaan-socodka ee bukaannada qaaxada sambabada ayaa sidoo kale sii waday inay soo roonaato, weli waxaa jira la'aanta daawooyinka ka hortagga qaaxada, iyo ifafaalaha isticmaalka daroogada aan macquul ahayn ee bukaan-socodka waa mid aad u sarreeya.Sida iska cad, qaaxada Mycobacterium ee bukaanada qaba qaaxada sambabada gabi ahaanba lama dili karo waqti ku haboon, taasoo aakhirka keenta in qofka bukaanka ah uu jidhkiisa iska caabiyo darajooyin kala duwan, siina dheerayso muddada cudurka, kordhisana khatarta dhimashada bukaanka.
Kanaalka
| Kanaalka | Kanaalka iyo Fluorophores | Dib u celinta falcelinta A | Dareen-celinta B | Dib u celinta falcelinta C |
| FAM Channel | Wariye: FAM, Quencher: Midna | rpoB 507-514 | rpoB 513-520 | 38KD iyo IS6110 |
| Kanaalka CY5 | Wariye: CY5, Quencher: Midna | rpoB 520-527 | rpoB 527-533 | / |
| HEX (VIC) Channel | Wariye: HEX (VIC), Quencher: Midna | Xakamaynta gudaha | Xakamaynta gudaha | Xakamaynta gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ Madow |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | xaako |
| CV | ≤5.0% |
| LoD | qaaxada mycobacterium 50 bakteeriya/mL nooca duurjoogta ah ee rifampicin: 2x103bakteeriyada/ml mutant homozygous: 2x103bakteeriyada/ml |
| Gaar ahaan | Waxay ogaataa qaaxada mycobacterium-ka duurjoogta ah iyo goobaha isku-bedelka hiddo-wadaha kale ee iska caabbinta daroogada sida katG 315G>C\A, InhA-15C> T, natiijooyinka baaritaanku waxay muujinayaan wax iska caabin ah oo loo yaqaan 'rifampicin', taas oo macnaheedu yahay inaysan jirin wax falcelin ah. |
| Qalabka lagu dabaqi karo: | SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah LightCycler480® Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Haddii aad isticmaasho Makro & Micro-Test DNA/RNA Qalabka Guud (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) Soosaarayaasha Nucleic Acid (HWTS-3006C, HWTS-3006B)) ama Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. si loo soo saaro, ku dar 200μL ee Xakamaynta Wanaagsan, xakamaynta taban iyo muunada xaakada ee la farsameeyey si si isku xigta loo tijaabiyo, oo lagu daro 10μL ee kontoroolka gudaha si gooni gooni ah loogu daro Kontoroolka Wanaagsan, xakamaynta taban iyo muunada xaakada ee la warshadeeyay si loo tijaabiyo, tallaabooyinka xiga waa in si adag loo fuliyo. sida waafaqsan tilmaamaha soo saarista.Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 100μL.
