Clostridium difficile toxin A/B hiddasidaha (C.diff)
Magaca alaabta
HWTS-OT031A Qalabka lagu ogaanayo Asiidhka Nucleic ee loogu talagalay Clostridium difficile sun A/B hiddasidaha (C.diff) (Fluorescence PCR)
Shahaado
CE
Epidemiology
Clostridium difficile (CD), gram-positive anaerobic sporogenic Clostridium difficile, waa mid ka mid ah cudurada ugu muhiimsan ee keena caabuqyada mindhicirka ee nosocomial. Kiliinik ahaan, qiyaastii 15% ~ 25% shubanka lidka ku ah bakteeriyada, 50% ~ 75% colitis antimicrobial-la xiriira iyo 95% ~ 100% ee cudurka 'pseudomembranous enteritis' waxaa keena caabuqa Clostridium difficile (CDI). Clostridium difficile waa cudur-sidaha shuruudaysan, oo ay ku jiraan noocyada sunta ah iyo noocyada aan sunta ahayn.
Kanaalka
| FAM | tcdAhiddo-wade |
| ROX | tcdBhiddo-wade |
| VIC/HEX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | saxaro |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/ml |
| Gaar ahaan | Isticmaal qalabkan si aad u ogaato cudurada kale ee mindhicirka sida Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic noocyada, Adenovirus, rotavirus, norovirus, influenza A fayraska, fayraska hargabka A, dhammaan natiijooyinka xun ee DNA. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamka ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ahFQD-96A,HangzhouTignoolajiyada bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Ku dar 180μL of lysozyme baffer si uu roobka (ku milo lysozyme ilaa 20mg/mL oo leh lysozyme diluent), pipette si aad si fiican isugu walaaqdo, una socodsiiso 37°C in ka badan 30 daqiiqo.180μL ee xakamaynta togan iyo xakamaynta taban ee isku xigta. Ku darso10μL ee kontoroolka gudaha ee muunada si loo tijaabiyo, xakamayn togan, iyo xakamaynta taban ee isku xigxiga, oo isticmaal Nukleic Acid Extraction or Purification Reagent (YDP302) ee Tiangen Biotech (Beijing) Co., Ltd. muunadda xigta ee soo saarista DNA, oo fadlan si adag u raac tilmaamaha loo isticmaalo tillaabooyin gaar ah. Isticmaal DNA-da/RNase H2O for elution, iyo mugga elution ee lagu taliyey waa 100μL.
Doorashada 2.
Qaado 1.5mL oo ah tuubada centrifuge-free RNase/DNase, oo ku dar 200μL ee xakamaynta togan iyo xakamaynta taban ee isku xigta. Ku darso10μL ee kontoroolka gudaha ee muunada si loo tijaabiyo, xakamayn togan, iyo xakamaynta taban ee isku xigxiga, oo isticmaal Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (30H). Soo saarista waa in lagu fuliyaa si adag oo waafaqsan tilmaamaha isticmaalka, iyo mugga sare ee lagu taliyey waa 80μL.
