Sunta Clostridium difficile ee hidda-wadaha A/B (C.diff)
Magaca alaabta
Qalabka Baadhista Aashitada Nukliyeerka ee HWTS-OT031A ee hidda-wadaha A/B ee sunta Clostridium difficile (C.diff) (Fluorescence PCR)
Shahaadada
CE
Cudurrada Faafa
Clostridium difficile (CD), oo ah nooc ka mid ah bakteeriyada anaerobic-ga ee gram-positive Clostridium difficile, waa mid ka mid ah cudurada ugu waaweyn ee keena caabuqyada mindhicirka ee nosocomial. Caafimaad ahaan, qiyaastii 15% ~ 25% shubanka la xiriira jeermiska dila, 50% ~ 75% colitis-ka la xiriira jeermiska dila iyo 95% ~ 100% enteritis-ka pseudomembranous waxaa sababa caabuqa Clostridium difficile (CDI). Clostridium difficile waa cudur keena xaalad, oo ay ku jiraan noocyada sunta iyo noocyada aan sunta ahayn.
Kanaal
| FAM | tcdAhiddo-side |
| ROX | tcdBhiddo-side |
| VIC/HEX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | saxaro |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Gaar ahaanshaha | Adeegso qalabkan si aad u ogaato jeermisyada kale ee xiidmaha sida Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Kooxda B Streptococcus, Clostridium difficile noocyada aan cudurka lahayn, Adenovirus, rotavirus, norovirus, fayraska influenza A, fayraska influenza B iyo DNA-da genomic-ga aadanaha, natiijooyinku dhammaantood waa taban yihiin. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah QuantStudio®5 Nidaam oo PCR-Waqtiga-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®Nidaamka PCR-ga ee Waqtiga-dhabta ah ee 480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus (FQD-96A，HangzhouTiknoolajiyada bayoolaji) Wareege Kulayl oo Waqtiga-dhabta ah MA-6000 (Suzhou Molarray Co., Ltd.) Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Ikhtiyaarka 1aad.
Ku dar 180μL oo ah lysozyme buffer qaybta soo baxda (ku qas lysozyme ilaa 20mg/mL oo leh lysozyme diluent), pipette si aad si fiican isugu qasto, oo ku socodsii heerkul ah 37°C in ka badan 30 daqiiqo. Qaado 1.5mL oo ah tuubada centrifuge-ka ee aan lahayn RNase/DNase, oo ku dar180μL oo ah xakameyn togan iyo xakameyn taban oo isku xigxigta. Ku dar10μL oo ah xakamaynta gudaha ee muunadda si loo tijaabiyo, xakamaynta togan, iyo xakamaynta taban ee taxanaha ah, oo isticmaal Soo-saarista Aashitada Nucleic ama Reagent Purification (YDP302) ee Tiangen Biotech (Beijing) Co., Ltd. si aad u hesho soo-saarista DNA-da muunadda xigta, fadlan si adag u raac tilmaamaha loogu talagalay isticmaalka tallaabooyin gaar ah. Isticmaal H-ka aan lahayn DNase/RNase2O ee loogu talagalay dheecaanka, mugga dheecaanka lagu taliyayna waa 100μL.
Ikhtiyaarka 2aad.
Qaado 1.5mL oo tuubo centrifuge ah oo aan lahayn RNase/DNase, oo ku dar 200μL oo ah kontorool togan iyo kontorool taban oo isku xigxiga. Ku dar10μL oo ah xakamaynta gudaha ee muunadda si loo tijaabiyo, xakamaynta togan, iyo xakamaynta taban ee taxanaha ah, oo isticmaal Qalabka Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Soo saarista waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha loo isticmaalo, mugga dheecaanka ee lagu taliyayna waa 80μL.
