Ku-dhowaad Clombridium Corpol Corxin A / B hiddo-wade (C.Diff)
Magaca wax soo saarka
HWTS-OT031A Xirmooyinka ogaanshaha ee HWTS-ot031a
Shahaado
CE
Nadiifin
Clombridium Chickensile (CD), gram-ton-tom-stom-sproridium sporogenium-ka ku-dhafan, waa mid ka mid ah cudurada ugu waaweyn ee sababa infekshinka xiidmaha ee noscomial. Caafimaad ahaan 15% ah oo shuban laxiriira, 50% ~ 75% ee loo yaqaan 'colitial la xiriira lidka-ku-lahaanshaha iyo 9% ~ 100% ee saafiga pseudomembermenbrans waxaa keena infakshanka iskuxirka (CDI). Clombtridium Exfficile waa jaangooyooyin shuruud leh, oo ay kujirto noocyo sunta ah iyo noocyo aan sun lahayn.
Kanaal
| Fa farqad | tcdawaddo wa eega |
| Rox | tcdbwaddo wa eega |
| Vic / hex | Kontoroolka gudaha |
Cayayaanka farsamada
| Bakhaar | ≤-18 ℃ |
| Shelf-Life | 12 bilood |
| Nooca muunadda | saxarada |
| Tt | ≤38 |
| CV | ≤5.0% |
| Dhigid | 200CFU / ML |
| Si gaar ah | U adeegso xirmadaan si aad u ogaato cudurada kale ee xiidmaha sida Escherichia Coli, Staphella, Vibriocorra, Vibrioscal, Noofriras, norovirus, fayraska infalawansada, bini-aadamka b DNA, Natiijooyinka ayaa dhammaantood taban yihiin. |
| Aaladaha lagu dabaqi karo | Waxaa lagu dabaqay biosystemy-ga ee lagu dabaqay 7500 nidaamyada waqtiga-dhabta ah ee PCR Waxaa lagu dabaqay bio-yariyada bio-yada lagu dabaqay 7500 oo si dhakhso leh u ah nidaamyada PCR-ga TiarStudio®5 Nidaamyada PCR-ka waqtiga-dhabta ah Nidaamyada Slan-96p ee waqtiga-labaad ee PCR (tikniyoolajiyadda caafimaadka ee Hongshi ee Co., Ltd.) Nalalka®480 Nidaamka PCR-ka dhabta ah Sadarka 9600 oo lagu daray nidaamka ogaanshaha waqtiga-labaad ee PCR-ka (Fqd-96a,HangzhouTeknolojiyadda bioer) MA-6000 DIIWAAN GELINTA EE MADAXWEYNAHA (SUZHOU MolARYADA CODKA, LTD.) Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Biorad Cfx Opus 96 Nidaamka PCR-ka waqtiga-dhabta ah |
Socodka shaqada
Xulashada 1.
Ku dar 180μl oo ah Lysozyme Buffer-ka safka (ku milagool ku dhaca 20mg / ml oo leh Lysozyme-ka 20MSMMy si ka badan 30 daqiiqo. oo ku dar180μl oo ah kontorool togan iyo xakamayn taban oo isku xigxiga ah. Xisaab isku darid10μl kontoroolka gudaha ee muunadda in la tijaabiyo, xakamaynta togan, iyo xakamaynta taban ee isku xigxiga, oo loo isticmaalo soo saarista aashitada nucvenic (btd biopher) co. soosaarka DNA-da ee xigta, iyo Fadlan si adag u raac tilmaamaha loo adeegsado tillaabooyin gaar ah. U adeegso DNACE / RNASE FREN HE2O Si aad uhesho, iyo mugga regalin-wareejinta lagu taliyey waa 100μl.
Xulashada 2.
Qaado 1.5ml oo ah rnase / dnase-free centrifuge tuubada, oo ku dar 200μl oo kontorool togan iyo xakamayn taban oo taxane ah. Xisaab isku darid10μl kontoroolka gudaha ee muunadda in la tijaabiyo, xakamaynta togan, iyo xakamaynta taban ee isku xigxiga, oo loo adeegsado imaatinka fayraska viral-ka (hwts-3004-32, hwts-3004-4- 96) iyo macro-macro-micro-micro-micro otomatoric otomator-ka nucleic acid (hwts-3006). Soosaarista waa in lagu sameeyaa si waafaqsan tilmaamaha loo adeegsado, iyo mugga hiigsiga lagula taliyo waa 80μl.
