Orientia tsutsugamushi Nucleic Acid
Magaca alaabta
HWTS-OT002-Orientia tsutsugamushi Qalabka Lagu Ogaado Asiidhka Nucleic-ka (Fluorescence PCR)
Epidemiology
Scrub typhus waa cudur qandho ba'an oo uu keeno Orientia tsutsugamushi (Ot). Orientia scrub typhus waa Gram-negative obligate intracellular microorganism. Orientia scrub typhus waxay ka tirsan tahay genus Orientia sida ay u kala horreeyaan Rickettsiales, qoyska Rickettsiaceae, iyo genus Orientia. Tiifowga xoqan waxaa inta badan lagu kala qaadaa qaniinyada dirxiga chigger oo sidda cudur-sidaha. Waxa kiliinikada lagu gartaa qandho sare oo degdeg ah, eschar, lymphadenopathy, hepatosplenomegaly, iyo leukopenia dhiiga durugsan, iwm. Xaaladaha daran, waxay keeni kartaa qoorgooyaha, beerka iyo kelyaha oo hawlgab, habdhisyada xubnaha badan ee shaqada, iyo xataa dhimasho.
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | shaybaarada serum |
| CV | ≤5.0% |
| LoD | 500 koobi/μL |
| Qalabka lagu dabaqi karo | Lagu dabaqi karo nooca I ogaanshaha reagent: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), Nidaamyada ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer), MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerarka Heerarka (Suzhou Molarray Co., Ltd.), BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah. Lagu dabaqi karo nooca II reagent ogaanshaha: EudemonTMAIO800 (HWTS-EQ007) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
Macro & Micro-Test DNA / RNA Kit (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in loo fuliyaa si waafaqsan tilmaamaha isticmaalka soo saaritaan rea. Mugga muunadda la soo saaray waa 200μL, mugga sareynta lagu taliyeyna waa 100μL.
