Orientia tsutsugamushi
Magaca alaabta
HWTS-OT002B Orientia tsutsugamushiXirmada Ashitada Nukliyeerka ah (Fluorescence PCR)
Epidemiology
Scrub typhus waa cudur qandho ba'an oo uu keeno Orientia tsutsugamushi (Ot). Orientia scrub typhus waa Gram-negative obligate intracellular microorganism. Orientia scrub typhus waxay ka tirsan tahay genus Orientia sida ay u kala horreeyaan Rickettsiales, qoyska Rickettsiaceae, iyo genus Orientia. Tiifowga xoqan waxaa inta badan lagu kala qaadaa qaniinyada dirxiga chigger oo sidda cudur-sidaha. Waxa kiliinikada lagu gartaa qandho sare oo degdeg ah, eschar, lymphadenopathy, hepatosplenomegaly, iyo leukopenia dhiiga durugsan, iwm. Xaaladaha daran, waxay keeni kartaa qoorgooyaha, beerka iyo kelyaha oo hawlgab, habdhisyada xubnaha badan ee shaqada, iyo xataa dhimasho.
Kanaalka
| FAM | Orientia tsutsugamushi |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | serum cusub |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/μL |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdega ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Nidaamka PCR-waqtiga dhabta ah Nidaamyada ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer) MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerkulbeegga ah (Suzhou Molarray Co., Ltd.) BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: Makro & Imtixaan-yarGuud ahaanQalabka DNA/RNA (HWTS-3019(kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in lagu sameeyaa si waafaqsan tilmaamaha isticmaalka reagent soo saarida this. Mugga muunadda la soo saaray waa 200µL, mugga sareynta lagu taliyeyna waa100µL
