Sideed Nooc Oo Fayras Neefsi Ah
Magaca alaabta
HWTS-RT184-Siddeed nooc oo ah Fayrasyada Nuclear-ka ee Ashitada lagu ogaado (Fluorescence PCR)
Epidemiology
Infekshanka hab-mareenka neef-mareenka waa nooca ugu badan ee cudurrada bani'aadamka, kaas oo ku dhici kara jinsiga, da'da iyo gobol kasta, waana mid ka mid ah sababaha ugu muhiimsan ee cudurrada iyo dhimashada adduunka.[1]. Cudurrada neef-mareenka ee caadiga ah waxaa ka mid ah fayraska hargabka A (IFV A), fayraska hargabka B (IFV B), fayraska syncytial neefta, adenovirus, fayraska metapneumovirus, rhinovirus, fayraska parainfluenza (I/II/III) iyo mycoplasma pneumoniae, iwm.[2,3]. Calaamadaha bukaan-socodka iyo calaamadaha uu keeno caabuqa mareenka neef-mareenka waa isku mid, laakiin infekshanka ay keenaan cudur-sidaha kala duwan wuxuu leeyahay habab daweyn oo kala duwan, saameyn daawaynta iyo habka cudurka[4,5]. Waqtigan xaadirka ah, hababka ugu muhiimsan ee shaybaarka lagu ogaanayo cudurada neef-mareenka waxaa ka mid ah: go'doominta fayraska, ogaanshaha antigen iyo ogaanshaha nucleic acid. Qalabkani waxa uu ogaanayaa oo aqoonsanayaa asiidhyada nukliyeerka fayraska ee gaarka ah ee shakhsiyaadka leh calaamadaha iyo calaamadaha caabuqa neef-mareenka, iyadoo lala kaashanayo natiijooyinka kale ee kiliinikada iyo shaybaarka si ay u caawiyaan ogaanshaha caabuqa fayraska neefsashada.
Qiyaasta Farsamada
| Kaydinta | 2-8℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Oropharyngeal suuf; Nasopharyngeal suuf |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 koobi/ml |
| Gaar ahaan | Falcelinta isdhaafsiga: Ma jiro falcelin iskutallaab ah oo u dhexeeya xirmada iyo fayraska Boca, Cytomegalovirus, Fayraska Epstein-Barr, fayraska herpes simplex, fayraska varicella zoster, fayraska qaamo-qashiir, Enterovirus, fayraska jadeecada, coronavirus-ka bini'aadamka, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Pneptococcusiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Qaaxada Mycobacterium, Gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptoanscoccus catarrh, Lactobacillus, Corynebacterium, DNA genomic aadanaha. Tijaabada faragelinta: Dooro mucin (60mg/mL), dhiiga dadka (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride oo leh 5% ilaaliye (20mg/ml), beclomethasone (20mg/mL), dexamethasone (20mg/ml), 20mg/ml (2mg/ml), budesonide (1mg/ml), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirom (1mg/mL), mupiromcin (20mg/mL), mupirocin (20mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Natiijadu waxay muujisay in walxaha soo dhexgala ee kor ku xusan |
| Qalabka lagu dabaqi karo | Lagu dabaqi karo nooca I ogaanshaha reagent: Applied Biosystems 7500 Nidaamyada PCR-waqtiga-dhabta ah, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus-waqtiga dhabta ah ee PCR Nidaamyada ogaanshaha (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler), Co.Rzhou Cycler Real-Time Nidaamka PCR, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah. Lagu dabaqi karo nooca II reagent ogaanshaha: EudemonTM AIO800 (HWTS-EQ007) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (kaaso loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), iyo Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8)TM AIO800 (HWTS-EQ007)) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Mugga muunadda la soo saaray waa 200μL iyo mugga sareynta lagu taliyey waa 150μL.
