La qaboojiyay 11 Nooc oo ah Cudurada Nucleic Acid
Magaca alaabta
HWTS-RT190 -Baraf-qalajiyey-Baraf-qalajiyey 11 Nooc oo ah Xashiishada Nucleiic Acid Qalabka Lagu Ogaado (Fluorescence PCR)
Epidemiology
Caabuqa mareenka neef-mareenka waa cudur muhiim ah oo si ba'an khatar ugu ah caafimaadka aadanaha. Daraasaduhu waxay muujiyeen in inta badan caabuqyada mareenka neef-mareenka ay sababaan bakteeriyada iyo/ama caabuqa fayrasyada kuwaas oo wada gala qofka martida loo yahay, taasoo keenta darnaanta cudurka ama xitaa dhimasho. Sidaa darteed, ogaanshaha cudur-sidaha waxay ku siin kartaa daaweyn la beegsanayo waxayna hagaajinaysaa heerka badbaadada bukaanka[1,2]. Si kastaba ha ahaatee, hababka dhaqameed ee lagu ogaanayo cudur-sidaha neef-mareenka waxaa ka mid ah baaritaanka microscopic, dhaqanka bakteeriyada, iyo baaritaanka difaaca jirka. Hababkani waa kuwo adag, waqti badan qaata, farsamo ahaan u baahan, oo dareenkoodu hooseeyo. Intaa waxaa dheer, ma ogaan karaan cudur-sideyaal badan oo hal muunad ah, taas oo adkeyneysa in la siiyo dhakhaatiirta ogaanshaha saxda ah ee caawimada. Natiijo ahaan, dawooyinka intooda badani waxay weli ku jiraan marxaladda daawaynta la taaban karo, taas oo aan kaliya dardargelinin wareegga caabbinta bakteeriyada, laakiin sidoo kale waxay saamaysaa ogaanshaha waqtiga bukaanka [3]. Hargabka Haemophilus ee caadiga ah, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae iyo Chhlaemia. pneumophila waa cudur-sidaha muhiimka ah ee keena caabuqyada neef-mareenka ee nosocomial [4,5]. Qalabkan shaybaadhku waxa uu ogaanayaa oo aqoonsanayaa asiidhyada nucleic-ka ee gaarka ah ee cudur-sidaha kor ku xusan ee shakhsiyaadka leh calaamadaha iyo calaamadaha caabuqa neef-mareenka, oo ay ku daraan natiijooyinka shaybaarka kale si ay u caawiyaan ogaanshaha caabuqa caabuqa neef-mareenka.
Qiyaasaha Farsamada
| Kaydinta | 2-30℃ |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | Dhuunta dhuunta |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD ee xirmada Klebsiella oof wareenka waa 500 CFU/ml; LoD ee Streptococcus pneumoniae waa 500 CFU/ml; LoD ee hargabka Haemophilus waa 1000 CFU/ml; LoD ee Pseudomonas aeruginosa waa 500 CFU/mL; LoD ee Acinetobacter baumannii waa 500 CFU/mL; LoD ee Stenotrophomonas maltophilia waa 1000 CFU/mL; LoD ee xiiq-dheerta Bordetella waa 500 CFU/ml; LoD of Bordetella parapertussis waa 500 CFU/ml; LoD of Mycoplasma pneumoniae waa 200 koobi/mL; LoD ee Legionella pneumophila waa 1000 CFU/mL; LoD of Chlamydia pneumoniae waa 200 koobi/mL. |
| Gaar ahaan | Ma jiraan wax falcelin ah oo u dhexeeya xirmada iyo cudur-sidaha kale ee neef-mareenka ee ka baxsan tirada ogaanshaha ee xirmada tijaabada, tusaale ahaan Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptonecoccus, Reptonecoccus eccuhooges Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivius, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Hargabka A, fayraska hargabka, fayraska hargabka. Aspergillus fumigatus, Candida glabrata, iyo Candida tropicalis. |
| Qalabka lagu dabaqi karo | Nooca I: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah, Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhaqsaha badan, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Nidaamyada PCR-waqtiga-dhabta ah, LineGene 9600 Plus-waqtiga-dhabta ah ee PCR Nidaamyada ogaanshaha (FQD-96A, farsamada Hangzhou Bioer), MA-6000-waqtiga dhabta ah ee heerkulka cabbirka (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, PCR-Time RealTime System, PCRFX, BioRad CFX96 Real-Time PCR System. Nooca II: EudemonTMAIO800 (HWTS-EQ007) ee Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Socodka Shaqada
Nooca I: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co. Qalabka.
