Isbeddelka Hidde-sidaha Isku-dhafka BCR-ABL ee Aadanaha
Magaca alaabta
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-GE010A-Human BCR-ABL (Fluorescence PCR)
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-GE016A ee Biyo-la'aanta ah ee Bini'aadamka BCR-ABL (Fluorescence PCR)
Cudurrada Faafa
Myelogenousleukemia daba-dheeraatay (CML) waa cudur ku dhaca unugyada asaasiga ah ee dhiigga. In ka badan 95% bukaannada CML waxay ku qaadaan koromosoomka Philadelphia (Ph) unugyadooda dhiig. Cudurka ugu badan ee CML waa sidan soo socota: Hidde-sidaha isku-darka BCR-ABL waxaa sameeya isbeddel u dhexeeya abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) oo ku yaal gacanta dheer ee koromosoomka 9 (9q34) iyo hidda-wadaha gobolka jabka (BCR) ee ku yaal gacanta dheer ee koromosoomka 22 (22q11); borotiinka isku-darka ee uu ku codeeyay hidda-wadahani wuxuu leeyahay firfircooni tyrosine kinase (TK), wuxuuna dhaqaajiyaa waddooyinka calaamadaha ee hoos u socda (sida RAS, PI3K, iyo JAK/STAT) si loo dhiirrigeliyo kala-qaybsanaanta unugyada loona joojiyo apoptosis-ka unugyada, taasoo ka dhigaysa unugyadu inay si xun u faafaan, sidaas darteedna ay sababaan dhacdada CML. BCR-ABL waa mid ka mid ah tilmaamayaasha ogaanshaha muhiimka ah ee CML. Isbeddelka firfircoon ee heerka qoraalka waa tilmaame lagu kalsoonaan karo oo lagu ogaan karo saadaasha cudurka leukemia waxaana loo isticmaali karaa in lagu saadaaliyo soo noqoshada cudurka leukemia ka dib daaweynta.
Kanaal
| FAM | Hidde-sidaha isku-dhafka BCR-ABL |
| VIC/HEX | Xakamaynta Gudaha |
Halbeegyada Farsamada
| Kaydinta | Dareere: ≤-18℃ Mugdi ku jira |
| Muddada khaanadaha | Dareere: 9 bilood |
| Nooca Muunada | Muunadaha dhuuxa lafta |
| LoD | 1000 Nuqul/ mL |
| Gaar ahaanshaha
| Ma jiro wax fal-celin ah oo isdhaafsi ah oo lala sameeyo hiddo-sidayaasha kale ee isku-dhafka ah TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, iyo PML-RARa. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee QuantStudio® 5-ta Waqti-dhabta ah Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
