Isbeddelka Hidda-sidaha BRAF ee Aadanaha V600E
Magaca alaabta
Qalabka Ogaanshada Isbeddelka HWTS-TM007-Human BRAF Hiddaha V600E (Fluorescence PCR)
Shahaadada
CE/TFDA
Cudurrada Faafa
In ka badan 30 nooc oo isbeddello BRAF ah ayaa la helay, kuwaas oo qiyaastii 90% ay ku yaalliin exon 15, halkaas oo isbeddelka V600E loo arko inuu yahay isbeddelka ugu badan, taas oo ah, thymine (T) oo ku taal booska 1799 ee exon 15 ayaa loo beddelaa adenine (A), taasoo keentay in valine (V) lagu beddelo booska 600 iyadoo la adeegsanayo glutamic acid (E) oo ku jirta wax soo saarka borotiinka. Isbeddellada BRAF waxaa badanaa laga helaa burooyinka halista ah sida melanoma, kansarka mindhicirka, kansarka qanjirka tayroodhka, iyo kansarka sambabka. Fahmidda isbeddelka hidda-wadaha BRAF waxay noqotay baahida loo qabo in la baaro daawooyinka bartilmaameedka u ah isbeddelka EGFR-TKIs iyo BRAF ee daaweynta daawada kiliinikada ee loogu talagalay bukaanada laga faa'iideysan karo.
Kanaal
| FAM | Isbeddelka V600E, xakamaynta gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | Shaybaarada unugyada bayoolojiga ee paraffin-ku-xidhan |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Adeegso xirmooyinka si aad u ogaato xakamaynta tayada LoD ee u dhiganta. a) marka la eego asalka nooca duurjoogta ah ee 3ng/μL, heerka isbeddelka 1% waxaa lagu ogaan karaa kaydka falcelinta si deggan; b) marka la eego heerka isbeddelka 1%, isbeddelka 1×103Nuqullo/mL oo ku jira asalka nooca duurjoogta ah ee 1×105Nuqullada/mL waxaa si joogto ah loogu ogaan karaa kaydka falcelinta; c) Kaydka Falcelinta IC wuxuu ogaan karaa xakamaynta tayada ugu hooseysa ee ogaanshaha SW3 ee xakamaynta gudaha ee shirkadda. |
| Qalabka Khuseeya: | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ahNidaamyada Bayoolajiga ee La Adeegsaday 7300 PCR-ka Waqtiga-dhabta ah Nidaamyada, QuantStudio® 5 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Unugyada DNA-da ee QIAamp ee QIAAmp (56404), Qalabka Soo saarista Degdegga ah ee DNA-da ee Paraffin-ku-xidhan (DP330) oo ay soo saartay Tiangen Biotech(Beijing) Co., Ltd.
