Bani'aadamka BRAF Hida V600E
Magaca alaabta
HWTS-TM007-Bani'aadamka BRAF Hida V600E Qalabka Ogaanshaha Isbedelka (Fluorescence PCR)
Shahaado
CE/TFDA
Epidemiology
In ka badan 30 nooc oo isbeddellada BRAF ah ayaa la helay, kuwaas oo qiyaastii 90% ay ku yaalliin exon 15, halkaas oo isbeddelka V600E loo arko inuu yahay isbeddelka ugu badan, taas oo ah, thymine (T) ee booska 1799 ee exon 15 ayaa loo beddelaa adenine (A), taasoo keentay beddelka borotiinka valine (V) ee booska 6. Isbeddellada BRAF waxaa caadi ahaan laga helaa burooyinka halista ah sida melanoma, kansarka mindhicirka, kansarka tayroodhka, iyo kansarka sanbabada. Fahamka beddelka hidda-wadaha BRAF waxay noqotay baahida loo qabo in lagu baadho EGFR-TKIs iyo BRAF dawooyinka beddelka hidda-wadaha ee daawaynta daawaynta ee lala beegsaday bukaannada laga faa'iidaysan karo.
Kanaalka
| FAM | Isbedelka V600E, xakamaynta gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | shaybaarada unugyada cudur-sidaha ee paraffin-ku-xidhan |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Isticmaal qalabka si aad u ogaato xakamaynta tayada LoD ee u dhiganta. a) ka hooseeya 3ng/μL asalka duurjoogta ah, 1% heerka isbeddelka ayaa lagu ogaan karaa falcelinta falcelinta; b) ka hooseeya 1% heerka isku-beddelka, isku-beddelka 1×103Koobiyo/ml ee asalka duurjoogta ah ee 1×105Nuqullada/ml waxaa si adag looga ogaan karaa kaydiyaha falcelinta; c) IC Reaction Buffer wuxuu ogaan karaa xakamaynta tayada ugu hooseysa ee SW3 ee kontoroolka gudaha ee shirkadda. |
| Qalabka lagu dabaqi karo: | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ahNidaamyada noolaha ee la dabaqay 7300 PCR-waqtiga-dhabta ah Nidaamyada, QuantStudio® 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: QIAGEN's QIAamp DNA FFPE Kit Tissue Kit (56404), Paraffin-ku-xidhan Tissue DNA-da Degdegga ah ee Soosaarka (DP330) ee ay soo saartay Tiangen Biotech(Beijing) Co., Ltd.
