Isbeddelka Hidde-sidaha Isku-dhafka EML4-ALK ee Aadanaha
Magaca alaabta
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-TM006-Human EML4-ALK (Fluorescence PCR)
Shahaadada
TFDA
Cudurrada Faafa
Qalabkan waxaa loo isticmaalaa in si tayo leh loogu ogaado 12 nooc oo isbeddel ah oo ah hidda-wadaha isku-dhafka EML4-ALK ee muunado bukaannada kansarka sambabada ee aan unugyada yaryar ahayn ee aadanaha in vitro. Natiijooyinka baaritaanka waa tixraac caafimaad oo keliya mana aha in loo isticmaalo saldhigga kaliya ee daaweynta shaqsiyeed ee bukaanada. Dhakhaatiirtu waa inay sameeyaan go'aanno dhammaystiran oo ku saabsan natiijooyinka baaritaanka iyagoo ku saleynaya arrimo ay ka mid yihiin xaaladda bukaanka, tilmaamaha daawada, jawaabta daaweynta, iyo tilmaamayaasha kale ee baaritaanka shaybaarka. Kansarka sambabada waa burooyinka ugu badan adduunka oo dhan, 80% ~ 85% kiisaska waa kansarka sambabada ee unugyada yaryar (NSCLC). Isku-darka hidda-wadaha ee borotiinka la xiriira microtubule-ka echinoderm microtubule-ka sida 4 (EML4) iyo anaplastic lymphoma kinase (ALK) waa bartilmaameed cusub oo NSCLC ah, EML4 iyo ALK waxay si kala duwan ugu yaalliin aadanaha kooxaha P21 iyo P23 ee koromosoomka 2 waxaana kala soocaya qiyaastii 12.7 milyan oo lammaane saldhig ah. Ugu yaraan 20 nooc oo isku-dhafan ayaa la helay, kuwaas oo ay ka mid yihiin 12-ka isku-dhafan ee ku jira Shaxda 1aad, halkaas oo mutant 1 (E13; A20) uu yahay kan ugu badan, oo ay ku xigto mutant 3a iyo 3b (E6; A20), oo ka dhigan qiyaastii 33% iyo 29% bukaanada qaba hidda-wadaha isku-dhafka EML4-ALK NSCLC, siday u kala horreeyaan. Daawooyinka ALK ee ay matalaan Crizotinib waa daawooyin bartilmaameedsanaya molecule-yada yaryar ee loo sameeyay isbeddellada isku-dhafka hidda-wadaha ALK. Iyagoo joojinaya dhaqdhaqaaqa gobolka ALK tyrosine kinase, xannibaya waddooyinka calaamadaynta aan caadiga ahayn ee hoos u socda, sidaas darteedna xannibaya koritaanka unugyada burooyinka, si loo gaaro daaweyn bartilmaameedsan oo loogu talagalay burooyinka. Daraasadaha kiliinikada ayaa muujiyay in Crizotinib uu leeyahay heer wax ku ool ah oo ka badan 61% bukaanada qaba isbeddellada isku-dhafka EML4-ALK, halka aysan wax saameyn ah ku yeelan bukaanada nooca duurjoogta ah. Sidaa darteed, ogaanshaha isbeddelka isku-dhafka EML4-ALK waa aasaaska iyo saldhigga hagitaanka isticmaalka daawooyinka Crizotinib.
Kanaal
| FAM | Bakhaarka falcelinta 1, 2 |
| VIC (HEX) | Kaydka falcelinta 2 |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 9 bilood |
| Nooca Muunada | unugyada bayoolojiga ee paraffin-ku-xidhan ama muunadaha qaybta |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Qalabkani wuxuu ogaan karaa isbeddellada isku-dhafka ilaa 20 nuqul. |
| Qalabka Khuseeya: | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee SLAN ®-96P Nidaamyada PCR-ga ee QuantStudio™ 5-ta Waqti-dhabta ah Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Qalabka soo saarista ee lagu taliyay: Qalabka RNeasy FFPE (73504) oo ay samaysay QIAGEN, Qalabka Soo saarista RNA ee qaybaha unugyada ee Paraffin-ku-xidhan (DP439) oo ay samaysay Tiangen Biotech(Beijing) Co.,Ltd.
