Isbeddellada KRAS 8
Magaca alaabta
HWTS-TM014-KRAS 8 Xirmada Garashada Isbeddellada (Fluorescence PCR)
HWTS-TM011-KRAS 8 Qalajiyaha Qabooji
Shahaado
CE/TFDA/Myanmar FDA
Epidemiology
Isbeddellada dhibcaha ee hiddo-wadaha KRAS ayaa laga helay dhowr nooc oo ah burooyinka bini'aadamka, qiyaastii 17% ~ 25% heerka beddelka ee burada, 15% ~ 30% heerka isbeddelka ee bukaannada kansarka sanbabada, 20% ~ 50% isbeddelka isbeddelka kansarka mindhicirka bukaanka.Sababtoo ah borotiinka P21 ee ku qoran hidda-wadaha K-ras wuxuu ku yaalaa dhinaca hoose ee dariiqa calaamadaynta EGFR, ka dib isbeddelka hidda-wadaha K-ras, dariiqa calaamadaynta hoose ayaa had iyo jeer shaqeeya oo aan saameyn ku yeelan daawooyinka kor lagu beegsanayo ee EGFR, taasoo keentay in si joogto ah kororka xun ee unugyada.Isbeddellada ku jira hidda-wadaha K-ras guud ahaan waxay iska caabbiyaan EGFR tyrosine kinase inhibitors ee bukaannada kansarka sambabada iyo iska caabbinta dawooyinka ka hortagga EGFR ee bukaannada kansarka mindhicirka.Sannadkii 2008, Shabakadda Kansarka Guud ee Qaranka (NCCN) waxay soo saartay hab-dhaqan caafimaad oo loogu talagalay kansarka mindhicirka, kaas oo tilmaamay in goobaha isbeddelka ee keena K-ras in la hawlgeliyo ay inta badan ku yaalliin codons 12 iyo 13 ee exon 2, waxayna ku talisay in Dhammaan bukaanada qaba kansarka mindhicirka ee metastatic waxaa laga baari karaa isbeddelka K-ras ka hor daawaynta.Sidaa darteed, ogaanshaha degdega ah ee saxda ah ee beddelka hidda-wadaha K-ras ayaa muhiimad weyn u leh hagidda daawaynta.Qalabkani waxa uu u isticmaalayaa DNA sida muunada ogaanshaha si uu u bixiyo qiimayn tayo leh oo ku saabsan heerka is bedelka, kaas oo ka caawin kara daaweeyayaasha baadhista kansarka mindhicirka, kansarka sanbabada iyo bukaanada kale ee buro ee ka faa'iidaysta daawooyinka la beegsaday.Natiijooyinka baaritaanka xirmada waxaa loogu talagalay tixraaca bukaan-socodka oo keliya mana aha in loo isticmaalo saldhigga keliya ee daaweynta gaarka ah ee bukaanka.Dhakhaatiirtu waa inay sameeyaan go'aanno dhammaystiran oo ku saabsan natiijooyinka baaritaanka oo ku saleysan arrimo ay ka mid yihiin xaaladda bukaanka, calaamadaha daroogada, jawaabta daaweynta iyo tilmaamayaasha kale ee baaritaanka shaybaarka.
Qiyaasaha Farsamada
| Kaydinta | Dareeraha: ≤-18℃ Madow;Lyophilized: ≤30℃ Madaw |
| Nolosha shelf | Dareeraha: 9 bilood;Lyophilized: 12 bilood |
| Nooca Tuunada | Unugyada cudur-sidaha ee paraffin-ku-xidhan ayaa ka kooban unugyo buro ah |
| CV | ≤5.0% |
| LoD | K-ras Reaction Buffer A iyo K-ras Reaction Buffer B waxay si adag u ogaan karaan 1% heerka isbeddelka ee ka hooseeya 3ng/μL asalka nooca duurjoogta ah |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7300 Nidaamyada PCR-waqtiga-dhabta ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler® 480 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Waxaa lagu talinayaa in la isticmaalo QIAGEN's QIAamp DNA FFPE Kit Tissue Kit (56404) iyo Paraffin-embedded Tissue DNA Repid Extraction Kit (DP330) oo ay soo saartay Tiangen Biotech(Beijing) Co., Ltd.
