Qalabka Ogaanshaha Isbeddellada KRAS 8 (Fluorescence PCR)-RUO
Magaca alaabta
Qalabka Ogaanshaha Isbeddellada KRAS 8 (Fluorescence PCR)-RUO
Cudurrada Faafa
Isbeddellada dhibcaha ee hidda-wadaha KRAS ayaa laga helay noocyo badan oo burooyinka aadanaha ah, qiyaastii 17% ~ 25% heerka isbeddelka burooyinka, 15% ~ 30% heerka isbeddelka ee bukaanada kansarka sambabada, 20% ~ 50% heerka isbeddelka ee bukaanada kansarka mindhicirka. Sababtoo ah borotiinka P21 ee lagu qeexay hidda-wadaha K-ras wuxuu ku yaal dhinaca hoose ee marinnada calaamadaynta EGFR, ka dib isbeddelka hidda-wadaha K-ras, marinnada calaamadaynta ee hoos u dhaca ayaa had iyo jeer la hawlgeliyaa mana saameeyaan daawooyinka la beegsanayo ee EGFR, taasoo keenta koror joogto ah oo unugyada ah. Isbeddellada ku dhaca hidda-wadaha K-ras guud ahaan waxay keenaan iska caabinta EGFR tyrosine kinase inhibitors ee bukaanada kansarka sambabada iyo iska caabbinta daawooyinka antibody-ga ee ka hortagga EGFR ee bukaanada kansarka mindhicirka [1, 2, 3]. Sannadkii 2008, Shabakadda Qaranka ee Kansarka Dhammaystiran (NCCN) waxay soo saartay tilmaamo ku saabsan daaweynta kansarka mindhicirka, kaas oo tilmaamay in goobaha isbeddelka keena ee K-ras la hawlgeliyo ay inta badan ku yaalliin codons 12 iyo 13 ee exon 2, waxayna ku talisay in dhammaan bukaanada qaba kansarka mindhicirka ee horumarsan ee metastatic metastatic laga baari karo isbeddelka K-ras ka hor daaweynta[4]. Sidaa darteed, ogaanshaha degdegga ah iyo saxda ah ee isbeddelka hidda-wadaha K-ras waa mid muhiimad weyn u leh hagitaanka daawada kiliinikada. Qalabkani wuxuu u adeegsadaa DNA-ga muunadda ogaanshaha si uu u bixiyo qiimeyn tayo leh oo ku saabsan xaaladda isbeddelka, taas oo ka caawin karta dhakhaatiirta baaritaanka kansarka mindhicirka, kansarka sambabada iyo bukaanada kale ee burooyinka ee ka faa'iidaysta daawooyinka la beegsanayo. Natiijooyinka baaritaanka ee qalabka waa tixraac caafimaad oo keliya mana aha in loo isticmaalo saldhigga kaliya ee daaweynta shaqsiyeed ee bukaanada. Dhakhaatiirtu waa inay sameeyaan go'aanno dhammaystiran oo ku saabsan natiijooyinka baaritaanka iyadoo lagu saleynayo arrimo ay ka mid yihiin xaaladda bukaanka, tilmaamaha daawada, jawaabta daaweynta iyo tilmaamayaasha kale ee baaritaanka shaybaarka.
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | qaybaha bayoolojiga ee paraffin-ku-xidhan ee aadanaha |
| CV | ≤5.0% |
| LoD | a) Kaydka Falcelinta K-ras A iyo Kaydka Falcelinta K-ras B waxay si deggan u ogaan karaan heerka isbeddelka 1% ee hoos yimaada asalka nooca duurjoogta ah ee 3ng/μL; b) Isbeddel 1×10 ah3Nuqullada/mL waxaa si joogto ah loogu ogaan karaa asalka duurjoogta ah ee 1×105Nuqullo/mL marka heerka isbeddelka uu yahay 1%; c) Marka shirkadda LoD reference SW3 la tijaabiyo, ma jiro qiimo Ct ama qiimaha Ct=0 ee Reaction Buffer A iyo Reaction Buffer B |
| Qalabka Khuseeya | Nidaamyada PCR-ka ee La Adeegsaday ee 7500 Real-Time, Nidaamyada PCR-ka ee La Adeegsaday ee 7300 Real-Time, Nidaamyada PCR-ka ee QuantStudio®5 Real-Time, Nidaamka PCR-ka ee LightCycler® 480 Real-Time, Nidaamka PCR-ka ee BioRad CFX96 Real-Time. |
Socodka Shaqada
Reagent-ka waa loo baahan yahay laakiin lama bixin:H2O aan lahayn DNase/RNase, ethanol aan biyo lahayn. Marka la tijaabinayo muunad unug oo paraffin ku jira, waxaa lagu talinayaa in la isticmaalo Qalabka Tissue DNA FFPE ee QIAGEN (56404) iyo Qalabka Soo-saarista Degdegga ah ee DNA-da ee Paraffin-ku-xidhan (DP330) oo ay soo saartay Tiangen Biotech(Beijing) Co., Ltd.
Alaabta la isticmaalo waa loo baahan yahay laakiin lama bixin:Talooyin aan lahayn DNase/RNase, galoofisyo la tuuri karo, Tuubo aan lahayn DNase/RNase, 8-tuubo oo xarig ah, centrifuge.
