Qalabka Ogaanshaha Aashitada Nukliyeerka ee Fayraska Dengue I/II/II/IV ee Qaboojiyaha lagu Qallajiyey (Fluorescence PCR)-RUO
Magaca alaabta
Qalabka Ogaanshaha Aashitada Nukliyeerka ee Fayraska Dengue I/II/II/IV ee Qaboojiyaha lagu Qallajiyey (Fluorescence PCR)-RUO
Cudurrada Faafa
Qandhada Dengue (DF), oo ay keento cudurka denguevirus (DENV), waa mid ka mid ah cudurrada ugu faafa ee arbovirus. Habka gudbinta waxaa ka mid ah Aedes aegypti iyo Aedes albopictus. DF waxay inta badan ku badan tahay meelaha kulaylaha iyo kuwa ka hooseeya. DENV waxay ka tirsan tahay flavivirus-ka hoostiisa flaviviridae, waxaana loo kala saari karaa 4 nooc oo serotypes ah iyadoo loo eegayo antigen-ka dusha sare. Calaamadaha kiliinikada ee caabuqa DENV waxaa ugu badan madax-xanuun, qandho, daciifnimo, ballaarinta qanjidhada limfa, leukopenia iwm., iyo dhiig-bax, shoog, dhaawac beerka ah ama xitaa dhimasho kiisaska daran. Sannadihii ugu dambeeyay, isbeddelka cimilada, magaalooyinka, horumarinta degdegga ah ee dalxiiska iyo arrimo kale ayaa bixiyay xaalado degdeg ah oo ku habboon gudbinta iyo faafitaanka DF, taasoo horseedaysa ballaarin joogto ah oo aagga faafa ee DF. Sidaa darteed, sameynta habka ogaanshaha cudurka ee DENV ee fudud, gaar ah oo degdeg ah waxay muhiim u tahay ogaanshaha kiliinikada ee DF.
Halbeegyada Farsamada
| Kaydinta | 2-8℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | seeram |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Nuqul/mL |
| Gaar ahaanshaha | Baaritaannadu waa kuwo taban oo laga helo muunadaha seeramka ee fayraska dengue; natiijooyinka baaritaanka faragelinta waxay muujinayaan marka fiirsashada bilirubin ee seeramka aysan ka badnayn 168.2μmol/mL, fiirsashada hemoglobin ee uu soo saaro hemolysis aysan ka badnayn 130g/L, fiirsashada dufanka dhiigga uusan ka badnayn 65mmol/mL, fiirsashada IgG ee guud ee seeramkana aysan ka badnayn 5mg/mL, ma jirto wax saameyn ah oo ku saabsan ogaanshaha fayraska dengue; isticmaal fayraska Hepatitis A, fayraska Hepatitis B, fayraska Hepatitis C, fayraska Herpes, fayraska equine encephalitis ee Eastern equine, fayraska Hantaan, fayraska Bunia, fayraska West Nile, fayraska Zika iyo muunadaha serumka genomic ee aadanaha si loogu sameeyo baaritaanka iskutallaabta-falcelinta, natiijooyinkuna waxay muujinayaan inaysan jirin wax falcelin ah oo u dhexeeya xirmadan iyo jeermisyada kor ku xusan. |
| Qalabka Khuseeya | Nooca I: Nidaamyada PCR-ka ee La Adeegsaday ee 7500 Real-Time, Nidaamyada PCR-ka ee QuantStudio®5 Real-Time, Nidaamyada PCR-ka ee SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time Nidaamka Ogaanshaha PCR (FQD-96A, Tiknoolajiyadda Hangzhou Bioer), MA-6000 Real-Time Wareegga Kulaylka Tirada leh (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time Nidaamka PCR, BioRad CFX Opus 96 Real-Time Nidaamka PCR. Nooca II: EudemonTMAIO800 (HWTS-EQ007) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Socodka Shaqada
Reagent-yadu waxay u baahan yihiin laakiin lama bixin:
Nooca I: Qalabka Guud ee DNA/RNA ee Macro & Micro-Test (HWTS-3017) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Nooca II: Qalabka Guud ee Baaritaanka Macro & Micro-Test DNA/RNA (HWTS-3023-8), waxaa loo isticmaali karaa EudemonTM AIO800 (HWTS-EQ007) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kahor inta aan la isticmaalin qalabka tijaabada Nooca II, macaamiishu waxay leeyihiin qalab wax soo saar oo taageera iyo qalab kor u qaadis ah, injineerkuna wuxuu hagi doonaa macaamiisha si uu u fuliyo hawlgalka tijaabada marka uu isticmaalayo markii ugu horreysay.
Qalabka la isticmaalo waa loo baahan yahay laakiin lama bixin:Talooyin bilaash ah oo DNase/RNase ah, galoofisyo la tuuri karo, DNase/RNase Tuubooyinka EP ee bilaashka ah, centrifuge, istaag birlab ah, xariijimo 8-tuubo ah oo loogu talagalay PCR.
